LOCUS       sequence-227775-       10184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA damage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Yang KS, Kohler RH, Landon M, Giedt R, Weissleder R
  TITLE     Single cell resolution in vivo imaging of DNA damage following PARP 
            inhibition.
  JOURNAL   Sci Rep. 2015 May 18;5:10129. doi: 10.1038/srep10129.
  PUBMED    25984718
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-05-18"; volume: "5:10129. doi"; pages: " 
            10.1038/srep10129"
COMMENT     Sequence Label: Apple-53BP1trunc
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2820..3527
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3233..3252
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5019..5518
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5054..5077)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5451..5470
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5539..6138
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5539..5558)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6035..6055
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6152..6740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6205..6225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6623..6634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6947..7580
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7709..7725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7709..7725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7722..7744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7733..7749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7757..7763,7764..7781,7782..7787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7802..7823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7940..7957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8111..8699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8191..8210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8870..9661,9662..9730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9493..9512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9731..9835)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9883..10017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9920..9939)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9974..9993
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccgggata gctagcacca
     2821 tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     2881 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     2941 gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct
     3001 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggtctac attaagcacc
     3061 cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg tgggagcgcg
     3121 tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc ctgcaggacg
     3181 gcgtgttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     3241 tgcagaagaa gaccatgggc tgggaggcct ccgaggagcg gatgtacccc gaggacggcg
     3301 ccctgaagag cgagatcaag aagaggctga agctgaagga cggcggccac tacgccgccg
     3361 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg
     3421 acatcaagtt ggacatcgtg tcccacaacg aggactacac catcgtggaa cagtacgaac
     3481 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga gaagaagagt
     3541 ttgatatgcc tcagcctcca catggccatg tcttacatcg tcacatgaga acaatccggg
     3601 aagtacgcac acttgtcact cgtgtcatta cagatgtgta ttatgtggat ggaacagaag
     3661 tagaaagaaa agtaactgag gagactgaag agccaattgt agagtgtcag gagtgtgaaa
     3721 ctgaagtttc cccttcacag actgggggct cctcaggtga cctgggggat atcagctcct
     3781 tctcctccaa ggcatccagc ttacaccgca catcaagtgg gacaagtctc tcagctatgc
     3841 acagcagtgg aagctcaggg aaaggagccg gaccactcag agggaaaacc agcgggacag
     3901 aacccgcaga ttttgcctta cccagctccc gaggaggccc aggaaaactg agtcctagaa
     3961 aaggggtcag tcagacaggg acgccagtgt gtgaggagga tggtgatgca ggccttggca
     4021 tcagacaggg agggaaggct ccagtcacgc ctcgtgggcg tgggcgaagg ggccgcccac
     4081 cttctcggac cactggaacc agagaaacag ctgtgcctgg ccccttgggc atagaggaca
     4141 tttcacctaa cttgtcacca gatgataaat ccttcagccg tgtcgtgccc cgagtgccag
     4201 actccaccag acgaacagat gtgggtgctg gtgctttgcg tcgtagtgac tctccagaaa
     4261 ttcctttcca ggctgctgct ggcccttctg atggcttaga tgcctcctct ccaggaaata
     4321 gctttgtagg gctccgtgtt gtagccaagt ggtcatccaa tggctacttt tactctggga
     4381 aaatcacacg agatgtcgga gctgggaagt ataaattgct ctttgatgat gggtacgaat
     4441 gtgatgtgtt gggcaaagac attctgttat gtgaccccat cccgctggac actgaagtga
     4501 cggccctctc ggaggatgag tatttcagtg caggagtggt gaaaggacat aggaaggagt
     4561 ctggggaact gtactacagc attgaaaaag aaggccaaag aaagtggtat aagcgaatgg
     4621 ctgtcatcct gtccttggag caaggaaaca gactgagaga gcagtatggg cttggcccct
     4681 atgaagcagt aacacctctt acaaaggcag cagatatcag cttagacaat ttggtggaag
     4741 ggaagcggaa acggcgcagt aacgtcagct ccccagccac ccctactgcc tccagtagca
     4801 gcagcacaac ccctacccga aagatcacag aaagtcctcg tgcctccatg ggagttctct
     4861 caggcaaaag aaaacttatc acttctgaag aggaacggtc ccctgccaag cgaggtcgca
     4921 agtctgccac agtaaaacct ggtgcagtag gggcaggaga gtttgtgagc ccctgtgaga
     4981 gtggagacaa caccgtgaat gatctagata attctaccgg gtaggggagg cgcttttccc
     5041 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     5101 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5161 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5221 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     5281 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     5341 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     5401 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     5461 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     5521 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5581 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5641 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5701 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5761 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5821 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5881 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5941 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6001 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6061 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6121 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6181 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6241 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6301 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6361 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6421 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6481 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6541 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6601 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6661 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6721 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     6781 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6841 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6901 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     6961 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7021 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7081 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7141 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7201 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7261 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7321 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7381 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7441 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7501 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7561 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7621 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     7681 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7921 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9841 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     9901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10021 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10081 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10141 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
