LOCUS       sequence-227781-       10308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with eGFP 
            replacing exon 2-3 retaining N-propeptide minor triple helix and 
            N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: eGFP-pro-alpha-2(I)-L
FEATURES             Location/Qualifiers
     source          1..10308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1039..1041,1042..1752)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1039..1041,1042..1752)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1081..1102)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1342..1361)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5902..5919)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5908..6132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6178..6606
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6265..6284)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6475..6496
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6615..6635)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6620..6949
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6800..6935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6862..6881
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7016..7810
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7070..7089)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7680..7699
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7984..8105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8021..8040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8075..8094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8154..8170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8154..8170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8167..8189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8178..8194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8202..8208,8209..8226,8227..8232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8247..8268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8385..8402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8556..9141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8636..8655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9312..10103,10104..10172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9935..9954
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10173..10277)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgta
      961 gacatgctca gctttgtgga tacgcggact ctgttgctgc ttgcagtaac ttcgtgccta
     1021 gcaacatgcc aagatatcgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1081 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     1141 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1201 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1261 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1321 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1381 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1441 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     1501 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1561 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1621 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1681 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1741 gagctgtaca agggccccac tggagacaga ggaccacgtg gacaaagggg tcccgcaggt
     1801 ccccgaggca gagatggtgt tgatggtccc atgggccctc ctggtccccc tggctcccct
     1861 ggtcctcccg gctcccctgc cccccctggt cttactggga actttgctgc tcagtattct
     1921 gacaaaggag tttcatctgg ccctggacca atgggtttaa tgggacccag aggccctcct
     1981 ggtgctgttg gagcccctgg ccctcaaggt ttccaaggac ctgctggtga acctggtgag
     2041 cctggtcaaa cgggtcctgc aggtccccgt ggtccagctg gctctcctgg caaggctggt
     2101 gaggatggtc accctggaaa acccggaaga cctggggaga gaggagtcgt tggaccacag
     2161 ggtgctcgtg gtttccccgg gactcctgga cttcctggct tcaaaggcgt gaaaggacac
     2221 agtggtatgg atggattaaa gggacagcct ggtgcacagg gtgtgaaggg tgaacccggt
     2281 gcccctggag agaatggaac tccaggtcaa gcaggagccc gagggcttcc tggtgagaga
     2341 gggcgtgtcg gagctcctgg tccagctggt gcccgaggca gtgatggaag cgttggcccc
     2401 gtcggccctg ctggtcccat tgggtctgct ggaccccctg gtttcccagg tgctcctggt
     2461 cccaagggtg aacttgggcc cgttggtaac cctggccctg ctggtcctgc tggtccccgt
     2521 ggtgaagtgg gtcttccagg tctttctggc cctgttggac ctcctggcaa ccctggaaca
     2581 aatgggctca ctggcgccaa gggtgctact ggactccctg gtgtcgctgg ggctcccggt
     2641 ctccctggcc cccgtggtat tcctggcccc gctggtgctg ctggtgctac aggtgccaga
     2701 ggacttgttg gtgagcctgg tcctgctggc tccaaaggag aatccggtaa caagggtgag
     2761 cctggctctg ttggagccca aggtcctcct ggtcccagcg gtgaagaagg aaagagaggg
     2821 tctcccggag aagctggatc tgctggccct gcagggcctc cagggcttag aggcagccct
     2881 ggttctcgag gtcttcctgg agctgatggc agagctggtg taatgggtcc tcctggcaat
     2941 cgtggttcaa ccggccctgc tggaatccga ggtcctaatg gagatgccgg tcgacctggg
     3001 gaacctggtc tcatgggacc cagaggtctt cctgggtctc ctggaaatgt tggcccatct
     3061 ggtaaagaag gccctgtggg tctccctggc attgatggca gacctggccc aatcggccca
     3121 gctggaccaa gaggtgaagc tggcaacatc ggattccctg gacccaaagg cccctctggt
     3181 gatcctggca aacctggtga gagaggacac cccggtcttg ctggtgctcg gggagctcca
     3241 ggacccgatg gcaacaatgg agctcagggc ccccctggac cccagggtgt tcaaggtggc
     3301 aaaggtgaac aaggccctgc tggtcctcct ggcttccagg gtctccctgg tccctcaggt
     3361 actactggag aagttggcaa gcccggagaa aggggtcttc ctggtgaatt cggtctccct
     3421 ggtcctgctg gtccaagagg agaacgtggt accccgggtg agagtggagc tgctggccct
     3481 tctggtccta ttggaagccg aggtcccagt ggagccccag ggcctgatgg aaacaagggt
     3541 gaagctggtg cagtcggtgc tccaggcagt gctggtgcct ctgggcctgg tgggcttcca
     3601 ggagagaggg gtgctgctgg catacctggg ggcaaaggag aaaagggtga aactggtctc
     3661 cgaggtgaca ctggcaacac tggtagagat ggtgctcgtg gcattcctgg tgctgtaggt
     3721 gcccctggtc ctgctggggc ctcaggtgac cggggtgaag ctggtgctgc cggtccttct
     3781 ggcccagctg gtcctcgggg tagccctggt gaacgtggtg aagttggccc tgctggcccc
     3841 aatggatttg ctggtcctgc tggtgctgct ggccaacccg gtgctaaagg agagaaggga
     3901 accaaagggc ctaagggtga aaatggcatt gttggtccaa ccgggtctgt tggagctgct
     3961 ggcccatctg gtcctaatgg cccacctggt cctgttggaa gtcgtggtga tggagggccc
     4021 cctggtatga ctggcttccc tggtgctgct ggacggactg gtccccccgg acccagtggt
     4081 attgctggcc cccctggccc ccctggtgct gctggaaagg aagggattcg tggtccccgt
     4141 ggcgaccaag gtccagttgg ccgaactgga gaaacaggtg catctggacc ccctggattt
     4201 gttggtgaaa agggtccctc tggagaacct ggtaccgcag gagcccctgg taccgcaggt
     4261 cctcagggtc ttcttggtgc tcccggtatt ctgggtctcc ctggctctag aggtgaacgt
     4321 ggtctgccag gtattgctgg tgctctgggt gaacctggtc ctctgggcat ctcaggccct
     4381 cctggtgccc gtggtccccc tggtgctgtg ggcagccctg gagttaatgg tgcccctggt
     4441 gaagctggtc gtgatggtaa ccctggcagc gatggtcccc caggtcgtga tggtcagcct
     4501 ggacacaagg gagagcgtgg ttaccctggc agcattggtc cgactggtgc tgcaggtgcc
     4561 cctggtcctc acggttctgt gggtcctgct gggaaacatg gaaaccgagg tgaacctggt
     4621 cctgccggtt ctgttggtcc tgttggtgct gttggtccaa gaggtcctag tggtcctcaa
     4681 ggcatccgag gtgacaaagg agaacctggt gataaagggc acaggggtct tcctggctta
     4741 aaaggataca gtggattgca gggtcttcct ggtcttgctg gcctacatgg tgaccaagga
     4801 gctcctggtc ctgtgggtcc tgctggtccc aggggtcctg ctgggccttc tggacccgtt
     4861 ggcaaagatg gtagatctgg gcaacctggc ccagtcgggc ctgctggtgt tcgtggttct
     4921 cagggtagcc aaggtcccgc tggtccccct ggtccccctg gacctcctgg tcctcctggt
     4981 gtcagcggag gtggctatga ctttggtttt gaaggagact tctacagggc tgaccagcct
     5041 cgctcccagc cttcactcag acccaaggac tatgaagttg atgcaactct gaaatctctc
     5101 aataaccaga ttgagaccct tctcactcct gaaggctcta gaaagaaccc tgctcgcacg
     5161 tgccgggact taagactcag ccacccagag tggaacagcg attactactg gattgaccct
     5221 aaccaaggat gtactatgga tgccatcaaa gtgtactgtg atttctctac tggtgaaacc
     5281 tgcatccagg cccaacctgt aaacacccca gcgaagaact catacagccg cgcccaggcc
     5341 aacaagcatg tctggttagg agagactatc aatggtggca gccagtttga atacaacgta
     5401 gaaggggtgt cctccaagga aatggcaact cagctcgcct tcatgcgcct gctagccaac
     5461 cgtgcttctc agaacatcac ctaccactgc aagaacagca ttgcgtacct ggatgaggag
     5521 acgggcagct tgaacaaggc tgtgcttctg cagggttcca acgatgttga acttgttgct
     5581 gagggcaaca gcaggttcac ctactctgtc ctagtcgatg gctgctccaa aaagacaaat
     5641 gaatggggca agacaatcat tgaatacaaa acaaataagc catctcgcct gccgttcctt
     5701 gacattgcac ctctggacat tggtggtgct gaccaagaat tccgtgtgga ggttggcccc
     5761 gtctgtttca aataagtgaa ctcaacataa attaaaaacc aaaaacccct gaaaaaactt
     5821 tctctttgcc gtttcctcct ttaaaaaaaa aaaaaaagcg gccgctcgag tctagagggc
     5881 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5941 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6001 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6061 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6121 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6181 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6241 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6301 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6361 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6421 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6481 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6541 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6601 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6721 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6901 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6961 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     7021 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     7081 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     7141 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     7201 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7261 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7321 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7381 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7441 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7501 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7561 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7621 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7681 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7741 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7801 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7861 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7921 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7981 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8101 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8161 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8221 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8281 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8341 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8401 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8461 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8521 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8581 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8641 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8701 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8761 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8821 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8881 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8941 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9001 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9061 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
     9121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9241 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9301 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9361 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9421 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9481 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9541 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9601 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9661 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9721 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9781 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9841 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9901 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9961 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10021 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10081 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10141 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10201 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10261 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
