LOCUS       sequence-227782-       10299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Type I procollagen alpha-2 chain (Col1a2) with 
            mApple replacing exon 2-3 retaining N-propeptide minor triple helix 
            and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott 
            C, Lippincott-Schwartz J, Leikin S
  TITLE     Noncanonical autophagy at ER exit sites regulates procollagen 
            turnover.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 
            10.1073/pnas.1814552115. Epub 2018 Oct 4.
  PUBMED    30287488
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1814552115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-10-23- 23"; volume: "115"; issue: "43"; pages: 
            "E10099-E10108"
COMMENT     Sequence Label: mApple-pro-alpha-2(I)-L
FEATURES             Location/Qualifiers
     source          1..10299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1039..1743
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1449..1468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5893..5910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5899..6123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6169..6597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6256..6275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6466..6487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6606..6626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6611..6940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6791..6926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6853..6872
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7007..7801
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7061..7080)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7671..7690
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7975..8096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8012..8031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8066..8085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8145..8161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8145..8161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8158..8180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8169..8185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8193..8199,8200..8217,8218..8223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8238..8259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8376..8393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8547..9132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8627..8646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9303..10094,10095..10163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9926..9945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10164..10268)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgta
      961 gacatgctca gctttgtgga tacgcggact ctgttgctgc ttgcagtaac ttcgtgccta
     1021 gcaacatgcc aagatatcgt gagcaagggc gaggagaata acatggccat catcaaggag
     1081 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     1141 ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa ggtgaccaag
     1201 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1261 gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt ccccgagggc
     1321 ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt taaccaggac
     1381 tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1441 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga ggagcggatg
     1501 taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct gaaggacggc
     1561 ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1621 ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga ctacaccatc
     1681 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1741 aagggcccca ctggagacag aggaccacgt ggacaaaggg gtcccgcagg tccccgaggc
     1801 agagatggtg ttgatggtcc catgggccct cctggtcccc ctggctcccc tggtcctccc
     1861 ggctcccctg ccccccctgg tcttactggg aactttgctg ctcagtattc tgacaaagga
     1921 gtttcatctg gccctggacc aatgggttta atgggaccca gaggccctcc tggtgctgtt
     1981 ggagcccctg gccctcaagg tttccaagga cctgctggtg aacctggtga gcctggtcaa
     2041 acgggtcctg caggtccccg tggtccagct ggctctcctg gcaaggctgg tgaggatggt
     2101 caccctggaa aacccggaag acctggggag agaggagtcg ttggaccaca gggtgctcgt
     2161 ggtttccccg ggactcctgg acttcctggc ttcaaaggcg tgaaaggaca cagtggtatg
     2221 gatggattaa agggacagcc tggtgcacag ggtgtgaagg gtgaacccgg tgcccctgga
     2281 gagaatggaa ctccaggtca agcaggagcc cgagggcttc ctggtgagag agggcgtgtc
     2341 ggagctcctg gtccagctgg tgcccgaggc agtgatggaa gcgttggccc cgtcggccct
     2401 gctggtccca ttgggtctgc tggaccccct ggtttcccag gtgctcctgg tcccaagggt
     2461 gaacttgggc ccgttggtaa ccctggccct gctggtcctg ctggtccccg tggtgaagtg
     2521 ggtcttccag gtctttctgg ccctgttgga cctcctggca accctggaac aaatgggctc
     2581 actggcgcca agggtgctac tggactccct ggtgtcgctg gggctcccgg tctccctggc
     2641 ccccgtggta ttcctggccc cgctggtgct gctggtgcta caggtgccag aggacttgtt
     2701 ggtgagcctg gtcctgctgg ctccaaagga gaatccggta acaagggtga gcctggctct
     2761 gttggagccc aaggtcctcc tggtcccagc ggtgaagaag gaaagagagg gtctcccgga
     2821 gaagctggat ctgctggccc tgcagggcct ccagggctta gaggcagccc tggttctcga
     2881 ggtcttcctg gagctgatgg cagagctggt gtaatgggtc ctcctggcaa tcgtggttca
     2941 accggccctg ctggaatccg aggtcctaat ggagatgccg gtcgacctgg ggaacctggt
     3001 ctcatgggac ccagaggtct tcctgggtct cctggaaatg ttggcccatc tggtaaagaa
     3061 ggccctgtgg gtctccctgg cattgatggc agacctggcc caatcggccc agctggacca
     3121 agaggtgaag ctggcaacat cggattccct ggacccaaag gcccctctgg tgatcctggc
     3181 aaacctggtg agagaggaca ccccggtctt gctggtgctc ggggagctcc aggacccgat
     3241 ggcaacaatg gagctcaggg cccccctgga ccccagggtg ttcaaggtgg caaaggtgaa
     3301 caaggccctg ctggtcctcc tggcttccag ggtctccctg gtccctcagg tactactgga
     3361 gaagttggca agcccggaga aaggggtctt cctggtgaat tcggtctccc tggtcctgct
     3421 ggtccaagag gagaacgtgg taccccgggt gagagtggag ctgctggccc ttctggtcct
     3481 attggaagcc gaggtcccag tggagcccca gggcctgatg gaaacaaggg tgaagctggt
     3541 gcagtcggtg ctccaggcag tgctggtgcc tctgggcctg gtgggcttcc aggagagagg
     3601 ggtgctgctg gcatacctgg gggcaaagga gaaaagggtg aaactggtct ccgaggtgac
     3661 actggcaaca ctggtagaga tggtgctcgt ggcattcctg gtgctgtagg tgcccctggt
     3721 cctgctgggg cctcaggtga ccggggtgaa gctggtgctg ccggtccttc tggcccagct
     3781 ggtcctcggg gtagccctgg tgaacgtggt gaagttggcc ctgctggccc caatggattt
     3841 gctggtcctg ctggtgctgc tggccaaccc ggtgctaaag gagagaaggg aaccaaaggg
     3901 cctaagggtg aaaatggcat tgttggtcca accgggtctg ttggagctgc tggcccatct
     3961 ggtcctaatg gcccacctgg tcctgttgga agtcgtggtg atggagggcc ccctggtatg
     4021 actggcttcc ctggtgctgc tggacggact ggtccccccg gacccagtgg tattgctggc
     4081 ccccctggcc cccctggtgc tgctggaaag gaagggattc gtggtccccg tggcgaccaa
     4141 ggtccagttg gccgaactgg agaaacaggt gcatctggac cccctggatt tgttggtgaa
     4201 aagggtccct ctggagaacc tggtaccgca ggagcccctg gtaccgcagg tcctcagggt
     4261 cttcttggtg ctcccggtat tctgggtctc cctggctcta gaggtgaacg tggtctgcca
     4321 ggtattgctg gtgctctggg tgaacctggt cctctgggca tctcaggccc tcctggtgcc
     4381 cgtggtcccc ctggtgctgt gggcagccct ggagttaatg gtgcccctgg tgaagctggt
     4441 cgtgatggta accctggcag cgatggtccc ccaggtcgtg atggtcagcc tggacacaag
     4501 ggagagcgtg gttaccctgg cagcattggt ccgactggtg ctgcaggtgc ccctggtcct
     4561 cacggttctg tgggtcctgc tgggaaacat ggaaaccgag gtgaacctgg tcctgccggt
     4621 tctgttggtc ctgttggtgc tgttggtcca agaggtccta gtggtcctca aggcatccga
     4681 ggtgacaaag gagaacctgg tgataaaggg cacaggggtc ttcctggctt aaaaggatac
     4741 agtggattgc agggtcttcc tggtcttgct ggcctacatg gtgaccaagg agctcctggt
     4801 cctgtgggtc ctgctggtcc caggggtcct gctgggcctt ctggacccgt tggcaaagat
     4861 ggtagatctg ggcaacctgg cccagtcggg cctgctggtg ttcgtggttc tcagggtagc
     4921 caaggtcccg ctggtccccc tggtccccct ggacctcctg gtcctcctgg tgtcagcgga
     4981 ggtggctatg actttggttt tgaaggagac ttctacaggg ctgaccagcc tcgctcccag
     5041 ccttcactca gacccaagga ctatgaagtt gatgcaactc tgaaatctct caataaccag
     5101 attgagaccc ttctcactcc tgaaggctct agaaagaacc ctgctcgcac gtgccgggac
     5161 ttaagactca gccacccaga gtggaacagc gattactact ggattgaccc taaccaagga
     5221 tgtactatgg atgccatcaa agtgtactgt gatttctcta ctggtgaaac ctgcatccag
     5281 gcccaacctg taaacacccc agcgaagaac tcatacagcc gcgcccaggc caacaagcat
     5341 gtctggttag gagagactat caatggtggc agccagtttg aatacaacgt agaaggggtg
     5401 tcctccaagg aaatggcaac tcagctcgcc ttcatgcgcc tgctagccaa ccgtgcttct
     5461 cagaacatca cctaccactg caagaacagc attgcgtacc tggatgagga gacgggcagc
     5521 ttgaacaagg ctgtgcttct gcagggttcc aacgatgttg aacttgttgc tgagggcaac
     5581 agcaggttca cctactctgt cctagtcgat ggctgctcca aaaagacaaa tgaatggggc
     5641 aagacaatca ttgaatacaa aacaaataag ccatctcgcc tgccgttcct tgacattgca
     5701 cctctggaca ttggtggtgc tgaccaagaa ttccgtgtgg aggttggccc cgtctgtttc
     5761 aaataagtga actcaacata aattaaaaac caaaaacccc tgaaaaaact ttctctttgc
     5821 cgtttcctcc tttaaaaaaa aaaaaaaagc ggccgctcga gtctagaggg cccgtttaaa
     5881 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5941 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6001 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6061 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6121 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6181 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6241 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6301 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6361 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6421 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6481 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6541 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6601 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6661 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6721 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6781 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6841 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6901 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6961 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7021 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7081 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7141 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7201 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7261 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7321 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7381 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7441 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7501 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7561 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7621 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7681 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7741 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7801 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7861 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7921 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7981 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8041 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8101 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9061 aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9121 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9181 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9241 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9301 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9361 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9421 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9481 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9541 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9601 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9661 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9721 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9781 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9841 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9901 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9961 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10021 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10081 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10141 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10201 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10261 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
