LOCUS       sequence-227784-       13266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13266)
  AUTHORS   Chai A, Mateus AM, Oozeer F, Sousa-Nunes R
  TITLE     Spatiotemporally controlled genetic perturbation for efficient 
            large-scale studies of cell non-autonomous effects.
  JOURNAL   Elife. 2018 Nov 27;7. pii: 38393. doi: 10.7554/eLife.38393.
  PUBMED    30479273
REFERENCE   2  (bases 1 to 13266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-11-27"; volume: "7. pii: 38393. doi"; pages: " 
            10.7554/eLife.38393"
COMMENT     Sequence Label: pDEST-Hemmar-DSCP-ftz-FLP(D)
FEATURES             Location/Qualifiers
     source          1..13266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    631..1016
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1050..1174
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1199..1204,1205..1222,1223..1229)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1283..1942
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1349..1368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2284..2589
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2468..2487
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2630..2754)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    5120..5505
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(5515..5533)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5985..6056,6458..6731,6806..7460,7522..7837,8041..8172,
                     8243..8857)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9636..10190,10191..10221)
                     /label=P element 5' end
     rep_origin      complement(10456..11044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10536..10555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11215..12006,12007..12075))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11838..11857
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12076..12180)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12248..12266
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12304..12326)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12426..12445
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    12654..12723
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 ctaggtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
      301 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      361 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      421 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      481 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      541 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      601 agccgaactg ttcgccaagc ttggctgcat cacgtaataa gtgtgcgttg aatttattcg
      661 caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa
      721 gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa
      781 atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg
      841 ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat
      901 accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca
      961 ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga tcaattgtgc
     1021 tcggcaacag catgctgcag cagatcatca caagtttgta caaaaaagct gaacgagaaa
     1081 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1141 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1201 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1261 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1321 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1381 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1441 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1501 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1561 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1621 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1681 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1741 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1801 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1861 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1921 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     1981 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2041 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2101 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2161 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2221 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2281 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2341 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2401 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2461 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2521 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2581 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2641 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2701 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatgat
     2761 ctggggccgg ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg
     2821 ctgagagcat cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta
     2881 atatcctttg aataagccaa ctttgaatca caagacgcat accaaacggt accgcggccg
     2941 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagccta ggttggcatc
     3001 aggtaggcat cacacacgat taacaacccc taaaaataca ctttgaaaat attgaaaata
     3061 tgtttttgta tacatttttg atattttcaa acaatacgca gttataaaac tcattagcta
     3121 acccattttt tctttgctta tgcttacaga ttgcaaagac ctgcaggctc gagaatcaaa
     3181 atgccgcagt ttgatatcct ctgcaagacc ccaccaaagg tgttggtgcg tcaattcgtg
     3241 gagcgatttg agaggccgtc gggtgagaag atcgccctgt gcgctgccga gttgacctat
     3301 ttgtgttgga tgatcactca taatggcacc gcgattaagc gcgctacctt tatgagctat
     3361 aacactatca ttagcaattc cctgtccttt gacatagtaa acaagtccct gcagtttaaa
     3421 tacaagactc agaaggccac tatattggag gcttcgctga aaaagttgat cccggcatgg
     3481 gagttcacga tcatcccata ctacggtcag aaacaccaga gcgatattac cgatattgta
     3541 agcagcctcc agctgcagtt tgagtccagc gaagaggctg ataagggtaa tagtcacagc
     3601 aaaaagatgc tgaaggcact gctgtccgag ggcgaaagca tctgggagat tactgagaaa
     3661 atcctgaact cgtttgagta caccagccga ttcaccaaga cgaagaccct gtaccagttc
     3721 ctctttttgg caaccttcat caattgtggt cgcttcagtg acatcaaaaa cgtggaccct
     3781 aaatcgttca agctggtgca gaataagtac ctgggtgtta tcatccagtg cttggttacc
     3841 gagactaaga cctccgtgtc caggcacatt tatttcttct ccgcaagggg tcgcatcgat
     3901 ccgctggtat acttggatga gttccttcgg aatagtgaac cagtcttgaa gcgcgtgaac
     3961 aggacgggca attccagtag caacaagcaa gagtaccagc tgctgaagga taatcttgtt
     4021 cggtcgtaca acaaagcctt gaagaaaaat gcgccatact cgatctttgc aatcaagaat
     4081 ggtcccaaga gtcatattgg ccggcacctg atgaccagtt tcttgtccat gaagggtctg
     4141 actgagctca caaacgtcgt tggcaactgg tcggacaagc gagcgagtgc cgtcgcccgc
     4201 acgacataca cccaccagat cacggccata ccagatcact atttcgcgct ggtgtcgcgt
     4261 tattatgcct atgatcccat cagcaaggag atgatcgcgc tgaaggacga aaccaatcca
     4321 atcgaggagt ggcagcatat cgagcaactt aagggaagcg ctgagggtag catccgttac
     4381 cccgcctgga acggcatcat cagccaggag gttctggatt acctgagctc ctacatcaat
     4441 cgccgtattt aatctagaat taattaacta agccagtcgg caatcggacg ccttcgaaac
     4501 atgcaacact aatgtttaat tcagatttca gcagagacaa gctaaaacac cgacgagttg
     4561 taatcatttc tgtgcgccag catatatttc ttatatacaa cgtaatacat aattatgtaa
     4621 ttctagcatc tccccaacac tcacatacaa acaaacaaaa aatacaaaca cacaaaacgt
     4681 atttacccgc acgcatcctt ggcgaggttg agtatgaaac aaaaacaaaa cttaatttag
     4741 agcaaagtaa ttacacgaat aaatttaata aaaaaaaact ataataaaaa cgccaatcat
     4801 gttatttcca aaaaaaaaaa aaaaaaaaaa actagacgag atgtttgcgc ttgtgtcgat
     4861 gaatgtaaag aattgcaact acatagatgg taattaattt cagctgtaag aagtacaagt
     4921 ttttcataat tgtgagaaaa gtgcagtagt atcgcttaaa gttcagtttt aatcagggca
     4981 tttagcagct cgaacccaat gtatttgtaa gcccaataaa cgtgtaaatg cctagagcaa
     5041 aatatcacaa ctgtagcttt tcatccgaaa gataataatg aatagactga cctgacgttt
     5101 ttcaaaatgc gtaactagtc acgtaataag tgtgcgttga atttattcgc aaaaacattg
     5161 catattttcg gcaaagtaaa attttgttgc ataccttatc aaaaaataag tgctgcatac
     5221 tttttagaga aaccaaataa ttttttattg catacccgtt tttaataaaa tacattgcat
     5281 accctctttt aataaaaaat attgcatact ttgacgaaac aaattttcgt tgcataccca
     5341 ataaaagatt attatattgc atacccgttt ttaataaaat acattgcata ccctctttta
     5401 ataaaaaata ttgcatacgt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     5461 ttatattgca taccttttct tgccatacca tttagccgat caattctagt atgtatgtaa
     5521 gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca
     5581 ctggatatca ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga
     5641 tgtaccaggt tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct
     5701 gtttgcctcc ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat
     5761 ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg
     5821 tcagcggttt cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg
     5881 tgccaaaact cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt
     5941 ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc
     6001 aggagctatt aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga
     6061 gtagtacatg tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc
     6121 aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac
     6181 aaatagtgac acgaaacaga ttattctggt agctgttctc gctatataag acaatttttg
     6241 agatcatatc atgatcaaga catctaaagg cattcatttt cgactatatt cttttttaca
     6301 aaaaatataa caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt
     6361 ataaacctac ttcgtaggat acttcggggt actttttgtt cggggttaga tgagcataac
     6421 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca
     6481 gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc ggccacccag
     6541 tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg
     6601 gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt
     6661 caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt
     6721 gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg
     6781 gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc
     6841 gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg
     6901 ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac
     6961 gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc
     7021 ctaacggcca gggaacacct gattttccaa gccatggtgc ggatgccacg acatctgacc
     7081 tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt
     7141 cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag
     7201 cgtctggcat tcgcctccga ggctctaacc gatccgccgc ttctgatctg cgatgagccc
     7261 acctccggac tggactcctt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg
     7321 cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc
     7381 tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa
     7441 gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct agtattacat
     7501 aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga
     7561 cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat
     7621 cgccaagata tgcgacaatt ttgccattag caaagtagcc cgggatatgg agcagttgtt
     7681 ggccaccaaa aatctggaga agccactgga gcagccggag aatgggtaca cctacaaggc
     7741 cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga
     7801 accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa
     7861 atgatataac gcttacaatt cttggaaaca aattcgctag attttagata gaattgcctg
     7921 attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaagat
     7981 aaataaattt catttaactc gcgaatatta atgagatgcg agtaacattt taatttgcag
     8041 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggtgtg
     8101 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     8161 gccacgataa atgtaagtca tgtttagaat acatttgcat ttcaataatt tactaacttt
     8221 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     8281 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattgcc
     8341 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     8401 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     8461 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     8521 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     8581 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     8641 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     8701 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     8761 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     8821 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     8881 cttgtttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     8941 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     9001 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
     9061 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
     9121 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
     9181 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
     9241 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggttcgctca aatggttccg
     9301 agtggttcat ttcgtctcaa tagaaattag taataaatat ttgtatgtac aatttatttg
     9361 ctccaatata tttgtatata tttccctcac agctatattt attctaattt aatattatga
     9421 ctttttaagg taattttttg tgacctgttc ggagtgatta gcgttacaat ttgaactgaa
     9481 agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt gggcataata
     9541 gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt taatttagaa
     9601 aatgcttgga tttcactgga actagaatta attcggctgc tgctctaaac gacgcatttc
     9661 gtactccaaa gtacgaattt tttccctcaa gctcttattt tcattaaaca atgaacagga
     9721 cctaacgcac agtcacgtta ttgtttacat aaatgatttt ttttactatt caaacttact
     9781 ctgtttgtgt actcccactg gtatagcctt cttttatctt ttctggttca ggctctatca
     9841 ctttactagg tacggcatct gcgttgagtc gcctcctttt aaatgtctga ccttttgcag
     9901 gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca aatttgggag ttttcaccaa
     9961 ggctgcaccc aaggctctgc tcccacaatt ttctcttaat agcacacttc ggcacgtgaa
    10021 ttaattttac tccagtcaca gctttgcagc aaaatttgca atatttcatt tttttttatt
    10081 ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac
    10141 aagcaaacgt gcactgaatt taagtgtata cttcggtaag cttcggctat cgacgggacc
    10201 accttatgtt atttcatcat gggccagacc cacgtagtcc agcggcagat cggcggcgga
    10261 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
    10321 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
    10381 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
    10441 caagaagaag gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    10501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    10561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    10621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    10681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    10741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    10801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    10861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    10921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    10981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    11161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    11221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    11341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    11401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    12001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    12061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    12121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    12181 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    12241 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    12301 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    12361 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    12421 ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacatgcc cgccgtgacc
    12481 gtcgagaacc cgctgacgct gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc
    12541 gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc
    12601 gcgttcgccg ggatcaacta ccgccacctc gacccgttca tcatgatgga ccagatgggt
    12661 gaggtggagt acgcgcccgg ggagcccaag ggcacgccct ggcacccgca ccgcggcttc
    12721 gagaccgtga cctacatcgt cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac
    12781 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
    12841 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
    12901 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
    12961 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
    13021 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
    13081 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
    13141 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
    13201 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
    13261 ccagac
//
