LOCUS       sequence-22780-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Hedgepeth CM, Deardorff MA, Rankin K, Klein PS
  TITLE     Regulation of glycogen synthase kinase 3beta and downstream Wnt 
            signaling by axin.
  JOURNAL   Mol Cell Biol. 1999 Oct . 19(10):7147-57.
  PUBMED    10490650
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Oct . 19(10):7147-57."
COMMENT     Sequence Label: Axin GID 1-2/pET29b
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             33..77
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             93..110
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             453..470
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(523..541)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      537..584
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(708..727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgtttaact ttaagaagga gatatacata tgaaagaaac cgctgctgct aaattcgaac
       61 gccagcacat ggacagccca gatctgggta ccctggtgcc acgcggttcc atggagagcg
      121 agcaacagag tatgtccagc gatgccgata ccatgtcatt aactgacagc agcgtagatg
      181 ggatcccacc gtacagactc cgaaaacact atcgcagaga aatgcaagag agtgccaacg
      241 caaatggtcg tggaccgcta cctcacattc ctcgtacata tcacatgcca aaggatattc
      301 acgtcgatcc tgaaaaattt gctgctgagc ttataagtcg cttggagggg gtcctgagag
      361 accgtgaagc agaacagaag cttgaagagc gacttaaacg cgtccgagct gaagaagaag
      421 gggatgatgg ggatgtctcc tctggactcg agcaccacca ccaccaccac tgagatccgg
      481 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      541 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      601 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      661 ggttacgcgc agcgngaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      721 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      781 ccctttaggg ttccgattta gtgctttacg gcacctcga
//
