LOCUS       sequence-227911-        9027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of 3xHA tagged human TERT, N-terminal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Venteicher AS, Meng Z, Mason PJ, Veenstra TD, Artandi SE
  TITLE     Identification of ATPases pontin and reptin as telomerase components
            essential for holoenzyme assembly.
  JOURNAL   Cell. 2008 Mar 21;132(6):945-57. doi: 10.1016/j.cell.2008.01.019.
  PUBMED    18358808
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.01"; journalName: "Cell"; date: "2008-03-21- 
            21"; volume: "132"; issue: "6"; pages: "945-57"
COMMENT     Sequence Label: pCDNA-3xHA-hTERT
FEATURES             Location/Qualifiers
     source          1..9027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      346..355
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             367..393
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             397..423
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             427..453
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             481..3876
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="PRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAA
                     FRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLD
                     GARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAP
                     SCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARR
                     RGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEA
                     TSLDGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQL
                     RPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH
                     AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPW
                     QVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCA
                     WLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYR
                     KSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNM
                     DYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVL
                     RVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGH
                     VRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRFM
                     CHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLL
                     VTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWC
                     GLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDLQ
                     VNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILKA
                     KNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLP
                     GTTLTALEAAANPALPSDFKTILD"
     CDS             3917..3946
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3962..3979
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4002..4019)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4008..4232
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4278..4706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4365..4384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4575..4596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4715..4735)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4720..5049
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4900..5035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4962..4981
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5116..5910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5170..5189)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5780..5799
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6086..6207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6123..6142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6177..6196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6256..6272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6256..6272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6269..6291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6280..6296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6304..6310,6311..6328,6329..6334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6349..6370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6487..6504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6658..7246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6738..6757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7417..8208,8209..8277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8040..8059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8278..8382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8457..8476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8648..9027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag cttggtaccg
      301 agctcggatc cactagtcca gtgtggtgga attctgcaga taaacgccac catggggcaa
      361 caaggatacc cgtatgatgt tccggattac gctggctacc catacgacgt cccagactac
      421 gctggctacc catacgacgt cccagactac gctcaaggag tgcggccgcg ggggcgcgcc
      481 ccgcgcgctc cccgctgccg agccgtgcgc tccctgctgc gcagccacta ccgcgaggtg
      541 ctgccgctgg ccacgttcgt gcggcgcctg gggccccagg gctggcggct ggtgcagcgc
      601 ggggacccgg cggctttccg cgcgctggtg gcccagtgcc tggtgtgcgt gccctgggac
      661 gcacggccgc cccccgccgc cccctccttc cgccaggtgt cctgcctgaa ggagctggtg
      721 gcccgagtgc tgcagaggct gtgcgagcgc ggcgcgaaga acgtgctggc cttcggcttc
      781 gcgctgctgg acggggcccg cgggggcccc cccgaggcct tcaccaccag cgtgcgcagc
      841 tacctgccca acacggtgac cgacgcactg cgggggagcg gggcgtgggg gctgctgctg
      901 cgccgcgtgg gcgacgacgt gctggttcac ctgctggcac gctgcgcgct ctttgtgctg
      961 gtggctccca gctgcgccta ccaggtgtgc gggccgccgc tgtaccagct cggcgctgcc
     1021 actcaggccc ggcccccgcc acacgctagt ggaccccgaa ggcgtctggg atgcgaacgg
     1081 gcctggaacc atagcgtcag ggaggccggg gtccccctgg gcctgccagc cccgggtgcg
     1141 aggaggcgcg ggggcagtgc cagccgaagt ctgccgttgc ccaagaggcc caggcgtggc
     1201 gctgcccctg agccggagcg gacgcccgtt gggcaggggt cctgggccca cccgggcagg
     1261 acgcgtggac cgagtgaccg tggtttctgt gtggtgtcac ctgccagacc cgccgaagaa
     1321 gccacctctt tggatggtgc gctctctggc acgcgccact cccacccatc cgtgggccgc
     1381 cagcaccacg cgggcccccc atccacatcg cggccaccac gtccctggga cacgccttgt
     1441 cccccggtgt acgccgagac caagcacttc ctctactcct caggcgacaa ggagcagctg
     1501 cggccctcct tcctactcag ctctctgagg cccagcctga ctggcgctcg gaggctcgtg
     1561 gagaccatct ttctgggttc caggccctgg atgccaggga ctccccgcag gttgccccgc
     1621 ctgccccagc gctactggca aatgcggccc ctgtttctgg agctgcttgg gaaccacgcg
     1681 cagtgcccct acggggtgct cctcaagacg cactgcccgc tgcgagctgc ggtcacccca
     1741 gcagccggtg tctgtgcccg ggagaagccc cagggctctg tggcggcccc cgaggaggag
     1801 gacacagacc cccgtcgcct ggtgcagctg ctccgccagc acagcagccc ctggcaggtg
     1861 tacggcttcg tgcgggcctg cctgcgccgg ctggtgcccc caggcctctg gggctccagg
     1921 cacaacgaac gccgcttcct caggaacacc aagaagttca tctccctggg gaagcatgcc
     1981 aagctctcgc tgcaggagct gacgtggaag atgagcgtgc gggactgcgc ttggctgcgc
     2041 aggagcccag gggttggctg tgttccggcc gcagagcacc gtctgcgtga ggagatcctg
     2101 gccaagttcc tgcactggct gatgagtgtg tacgtcgtcg agctgctcag gtctttcttt
     2161 tatgtcacgg agaccacgtt tcaaaagaac aggctctttt tctaccggaa gagtgtctgg
     2221 agcaagttgc aaagcattgg aatcagacag cacttgaaga gggtgcagct gcgggagctg
     2281 tcggaagcag aggtcaggca gcatcgggaa gccaggcccg ccctgctgac gtccagactc
     2341 cgcttcatcc ccaagcctga cgggctgcgg ccgattgtga acatggacta cgtcgtggga
     2401 gccagaacgt tccgcagaga aaagagggcc gagcgtctca cctcgagggt gaaggcactg
     2461 ttcagcgtgc tcaactacga gcgggcgcgg cgccccggcc tcctgggcgc ctctgtgctg
     2521 ggcctggacg atatccacag ggcctggcgc accttcgtgc tgcgtgtgcg ggcccaggac
     2581 ccgccgcctg agctgtactt tgtcaaggtg gatgtgacgg gcgcgtacga caccatcccc
     2641 caggacaggc tcacggaggt catcgccagc atcatcaaac cccagaacac gtactgcgtg
     2701 cgtcggtatg ccgtggtcca gaaggccgcc catgggcacg tccgcaaggc cttcaagagc
     2761 cacgtctcta ccttgacaga cctccagccg tacatgcgac agttcgtggc tcacctgcag
     2821 gagaccagcc cgctgaggga tgccgtcgtc atcgagcaga gctcctccct gaatgaggcc
     2881 agcagtggcc tcttcgacgt cttcctacgc ttcatgtgcc accacgccgt gcgcatcagg
     2941 ggcaagtcct acgtccagtg ccaggggatc ccgcagggct ccatcctctc cacgctgctc
     3001 tgcagcctgt gctacggcga catggagaac aagctgtttg cggggattcg gcgggacggg
     3061 ctgctcctgc gtttggtgga tgatttcttg ttggtgacac ctcacctcac ccacgcgaaa
     3121 accttcctca ggaccctggt ccgaggtgtc cctgagtatg gctgcgtggt gaacttgcgg
     3181 aagacagtgg tgaacttccc tgtagaagac gaggccctgg gtggcacggc ttttgttcag
     3241 atgccggccc acggcctatt cccctggtgc ggcctgctgc tggatacccg gaccctggag
     3301 gtgcagagcg actactccag ctatgcccgg acctccatca gagccagtct caccttcaac
     3361 cgcggcttca aggctgggag gaacatgcgt cgcaaactct ttggggtctt gcggctgaag
     3421 tgtcacagcc tgtttctgga tttgcaggtg aacagcctcc agacggtgtg caccaacatc
     3481 tacaagatcc tcctgctgca ggcgtacagg tttcacgcat gtgtgctgca gctcccattt
     3541 catcagcaag tttggaagaa ccccacattt ttcctgcgcg tcatctctga cacggcctcc
     3601 ctctgctact ccatcctgaa agccaagaac gcagggatgt cgctgggggc caagggcgcc
     3661 gccggccctc tgccctccga ggccgtgcag tggctgtgcc accaagcatt cctgctcaag
     3721 ctgactcgac accgtgtcac ctacgtgcca ctcctggggt cactcaggac agcccagacg
     3781 cagctgagtc ggaagctccc ggggacgacg ctgactgccc tggaggccgc agccaacccg
     3841 gcactgccct cagacttcaa gaccatcctg gactgattaa ttaagtctag agtcgagtct
     3901 agagggcccg cggttcgaac aaaaactcat ctcagaagag gatctgaata tgcataccgg
     3961 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
     4021 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4081 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4141 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4201 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4261 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4321 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4381 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4441 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4501 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4561 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4621 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4681 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     4741 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4801 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4861 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4981 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5041 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     5101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5281 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5641 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5881 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga
     5941 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6001 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6061 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6181 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8401 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     8461 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     8521 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     8581 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     8641 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     8701 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     8761 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     8821 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8881 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     8941 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9001 tacgtattag tcatcgctat taccatg
//
