LOCUS       sequence-227926-        8855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TAR cloning in yeast and low-copy maintenance of large constructs in
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP-BAC
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     781..797
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     782..798
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             799..1602
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(1611..1627)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1611..1627)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      1733..1952
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2043..2798
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     CDS             3193..4368
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4368..5339
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5412..5885
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             6279..7073
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6333..6352)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6943..6962
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7152..7655
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7696..7714
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7752..7774)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7874..7893
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7917..8132
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             8133..8795
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 tcagccatcc gcttgccctc atctgttacg ccagctcgga attaaccctc actaaaggga
       61 acaaaagctg gtaccgggcc ccctacaaca actaagaaaa tggctatcat gcggaagctg
      121 gtgagaagaa cagcatcggg acaagggaag gaagaacaaa gacaaagaaa acaaaagaaa
      181 gcaattgaaa acaaaacaaa acaattttca ttccttctct tatcattcct tttcttttct
      241 tttctctcat tcaacgcact ccatcgtatc cgtattcctc ttattttttc tctttctcta
      301 tatccatttc tttctctcta ggtgtgtcct ctctctctct tcaatttctc tactccgcat
      361 tccaacgcat ccttccccca acctcccatt tcctccttac ggcccgatag cgatcgtctt
      421 tccctcgcta tcactcgcta ccggcccctc ctctgcaccg taacctccta cgtatttacc
      481 atatcataaa gttttttccg acgcttatcg ctgaccccct gtcgccctcc tattggcttc
      541 cggattatct tcttgtccat aaggtgatcc atgcttcctg aagattcccg aaatgtgtcc
      601 actttggcgg ggaatcattc catccacttc tttctctctc gctttcctca ttcggcgctc
      661 cccttccgcg tctcattggt cttccgctcc gtttttgctt tgccgatgtt acttggggag
      721 aggtgcgata atcctttcgc aaaaactcgg tttgacgcct cccatggtat aaatagtggc
      781 tcgaggtcga cggtatcgat gtcgaaagct acatataagg aacgtgctgc tactcatcct
      841 agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct
      901 tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa
      961 atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt
     1021 aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt
     1081 gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa
     1141 tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag
     1201 caggcggcgg aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca
     1261 tgcaagggct ccctagctac tggagaatat actaagggta ctgttgacat tgcgaagagc
     1321 gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt
     1381 tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt
     1441 caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga
     1501 agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca
     1561 ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgg gatccactag
     1621 ttctagagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag aggaagcacc
     1681 agggaacagc acttatatat tctgcttaca cacgatgcct gaaaaaactt cccttggggt
     1741 tatccactta tccacgggga tatttttata attatttttt ttatagtttt tagatcttct
     1801 tttttagagc gccttgtagg cctttatcca tgctggttct agagaaggtg ttgtgacaaa
     1861 ttgccctttc agtgtgacaa atcaccctca aatgacagtc ctgtctgtga caaattgccc
     1921 ttaaccctgt gacaaattgc cctcagaaga agctgttttt tcacaaagtt atccctgctt
     1981 attgactctt ttttatttag tgtgacaatc taaaaacttg tcacacttca catggatctg
     2041 tcatggcgga aacagcggtt atcaatcaca agaaacgtaa aaatagcccg cgaatcgtcc
     2101 agtcaaacga cctcactgag gcggcatata gtctctcccg ggatcaaaaa cgtatgctgt
     2161 atctgttcgt tgaccagatc agaaaatctg atggcaccct acaggaacat gacggtatct
     2221 gcgagatcca tgttgctaaa tatgctgaaa tattcggatt gacctctgcg gaagccagta
     2281 aggatatacg gcaggcattg aagagtttcg cggggaagga agtggttttt tatcgccctg
     2341 aagaggatgc cggcgatgaa aaaggctatg aatcttttcc ttggtttatc aaacgtgcgc
     2401 acagtccatc cagagggctt tacagtgtac atatcaaccc atatctcatt cccttcttta
     2461 tcgggttaca gaaccggttt acgcagtttc ggcttagtga aacaaaagaa atcaccaatc
     2521 cgtatgccat gcgtttatac gaatccctgt gtcagtatcg taagccggat ggctcaggca
     2581 tcgtctctct gaaaatcgac tggatcatag agcgttacca gctgcctcaa agttaccagc
     2641 gtatgcctga cttccgccgc cgcttcctgc aggtctgtgt taatgagatc aacagcagaa
     2701 ctccaatgcg cctctcatac attgagaaaa agaaaggccg ccagacgact catatcgtat
     2761 tttccttccg cgatatcact tccatgacga caggatagtc tgagggttat ctgtcacaga
     2821 tttgagggtg gttcgtcatc attgatgagg gttgattatc acagtttatt actctgaatt
     2881 ggctatccgc gtgtgtacct ctacctggag tttttcccac ggtggatatt tcttcttgcg
     2941 ctgagcgtaa gagctatctg acagaacagt tcttctttgc ttcctcgcca gttcgctcgc
     3001 tatgctcggt tacacggctg cggcgagcgc tagtgataat aagtgactga ggtatgtgct
     3061 cttcttatct ccttttgtag tgttgctctt attttaaaca actttgcggt tttttgatga
     3121 ctttgcgatt ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc
     3181 aatgattaaa ggatgttcag aatgaaactc atggaaacac ttaaccagtg cataaacgct
     3241 ggtcatgaaa tgacgaaggc tatcgccatt gcacagttta atgatgacag cccggaagcg
     3301 aggaaaataa cccggcgctg gagaataggt gaagcagcgg atttagttgg ggtttcttct
     3361 caggctatca gagatgccga gaaagcaggg cgactaccgc acccggatat ggaaattcga
     3421 ggacgggttg agcaacgtgt tggttataca attgaacaaa ttaatcatat gcgtgatgtg
     3481 tttggtacgc gattgcgacg tgctgaagac gtatttccac cggtgatcgg ggttgctgcc
     3541 cataaaggtg gcgtttacaa aacctcagtt tctgttcatc ttgctcagga tctggctctg
     3601 aaggggctac gtgttttgct cgtggaaggt aacgaccccc agggaacagc ctcaatgtat
     3661 cacggatggg taccagatct tcatattcat gcagaagaca ctctcctgcc tttctatctt
     3721 ggggaaaagg acgatgtcac ttatgcaata aagcccactt gctggccggg gcttgacatt
     3781 attccttcct gtctggctct gcaccgtatt gaaactgagt taatgggcaa atttgatgaa
     3841 ggtaaactgc ccaccgatcc acacctgatg ctccgactgg ccattgaaac tgttgctcat
     3901 gactatgatg tcatagttat tgacagcgcg cctaacctgg gtatcggcac gattaatgtc
     3961 gtatgtgctg ctgatgtgct gattgttccc acgcctgctg agttgtttga ctacacctcc
     4021 gcactgcagt ttttcgatat gcttcgtgat ctgctcaaga acgttgatct taaagggttc
     4081 gagcctgatg tacgtatttt gcttaccaaa tacagcaata gtaatggctc tcagtccccg
     4141 tggatggagg agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt
     4201 gaaacggatg aagttggtaa aggtcagatc cggatgagaa ctgtttttga acaggccatt
     4261 gatcaacgct cttcaactgg tgcctggaga aatgctcttt ctatttggga acctgtctgc
     4321 aatgaaattt tcgatcgtct gattaaacca cgctgggaga ttagataatg aagcgtgcgc
     4381 ctgttattcc aaaacatacg ctcaatactc aaccggttga agatacttcg ttatcgacac
     4441 cagctgcccc gatggtggat tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg
     4501 ccattacttt gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg
     4561 atagtgttga gaagacctct cgggtatggt caggtaatga acgtgaccag gagctgctta
     4621 ctgaggacgc actggatgat ctcatccctt cttttctact gactggtcaa cagacaccgg
     4681 cgttcggtcg aagagtatct ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag
     4741 ctgctgcact taccgaaagt gattatcgtg ttctggttgg cgagctggat gatgagcaga
     4801 tggctgcatt atccagattg ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc
     4861 agcgttatgc aagccgattg cagaatgaat ttgctggaaa tatttctgcg ctggctgatg
     4921 cggaaaatat ttcacgtaag attattaccc gctgtatcaa caccgccaaa ttgcctaaat
     4981 cagttgttgc tcttttttct caccccggtg aactatctgc ccggtcaggt gatgcacttc
     5041 aaaaagcctt tacagataaa gaggaattac ttaagcagca ggcatctaac cttcatgagc
     5101 agaaaaaagc tggggtgata tttgaagctg aagaagttat cactctttta acttctgtgc
     5161 ttaaaacgtc atctgcatca agaactagtt taagctcacg acatcagttt gctcctggag
     5221 cgacagtatt gtataagggc gataaaatgg tgcttaacct ggacaggtct cgtgttccaa
     5281 ctgagtgtat agagaaaatt gaggccattc ttaaggaact tgaaaagcca gcaccctgat
     5341 gcgaccacgt tttagtctac gtttatctgt ctttacttaa tgtcctttgt tacaggccag
     5401 aaagcataac tggcctgaat attctctctg ggcccactgt tccacttgta tcgtcggtct
     5461 gataatcaga ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
     5521 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     5581 cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta
     5641 gtctgggacc atggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc
     5701 cactcgtatc gtcggtctga ttattagtct ggaaccacgg tcccactcgt atcgtcggtc
     5761 tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga
     5821 ccacgatccc actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta
     5881 ttgtcgatca gactatcagc gtgagactac gattccatca atgcctgtca agggcaagta
     5941 ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct
     6001 gtggattgct gctgtgtcct gcttatccac aacattttgc gcacggttat gtggacaaaa
     6061 tacctggtta cccaggccgt gccggcactg ggcttacatg gcgatagcta gactgggcgg
     6121 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     6181 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     6241 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6301 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6361 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6421 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6481 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6541 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6601 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6661 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6721 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6781 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6841 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6901 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6961 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     7021 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggttaac
     7081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7141 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
     7201 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
     7261 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
     7321 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
     7381 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
     7441 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
     7501 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
     7561 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
     7621 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg
     7681 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     7741 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     7801 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     7861 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatac caccttttca
     7921 attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt
     7981 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     8041 tacgcatatg tagtgttgag gaaacatgaa attgcccagt attcttaacc caactgcaca
     8101 gaacaaaaac ctgcaggaaa cgaagataaa tcatgacaga gcagaaagcc ctagtaaagc
     8161 gtattacaaa tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga
     8221 tagagcactc gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat
     8281 cgcaagtgat taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg
     8341 ccaagcattc cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc
     8401 atcacaccac tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctagggg
     8461 ccgtgcgtgg agtaaaaagg tttggatcag gatttgcgcc tttggatgag gcactttcca
     8521 gagcggtggt agatctttcg aacaggccgt acgcagttgt cgaacttggt ttgcaaaggg
     8581 agaaagtagg agatctctct tgcgagatga tcccgcattt tcttgaaagc tttgcagagg
     8641 ctagcagaat taccctccac gttgattgtc tgcgaggcaa gaatgatcat caccgtagtg
     8701 agagtgcgtt caaggctctt gcggttgcca taagagaagc cacctcgccc aatggtacca
     8761 acgatgttcc ctccaccaaa ggtgttctta tgtagaaaac tgtattataa gtaaatgcat
     8821 gtatactaaa ctcacaaatt agagcttcaa tttaa
//
