LOCUS       sequence-227928-        8008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-cas9 targeting in E. coli with ampicillin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pJZ002
FEATURES             Location/Qualifiers
     source          1..8008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(117..1067)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1115..1337
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(join(1959..2750,2751..2819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2582..2601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2820..2924)
                     /gene="bla"
                     /label=AmpR promoter
     misc_RNA        complement(3084..3162)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             3458..7564
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3458..7561
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    7585..7716
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   7717..7752
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   7783..7818
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 taagctagcc catgggtatg gacagttttc cctttgatat gtaacggtga acagttgttc
       61 tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag
      121 atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat
      181 gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa
      241 ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttacgt
      301 aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg
      361 ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta
      421 tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct
      481 ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca
      541 agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc
      601 ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa
      661 gacttaacat gttccagatt atattttatg aattttttta actggaaaag ataaggcaat
      721 atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac
      781 tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc
      841 tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga
      901 gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg
      961 gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag
     1021 cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg
     1081 tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc
     1141 cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta
     1201 aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt
     1261 caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc
     1321 agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa
     1381 acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc
     1441 gcaagctcgg ttgcggccgc aatcgggcaa atcgctgaat attccttttg tctccgacca
     1501 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     1561 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     1621 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     1681 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     1741 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     1801 gtatcatcaa cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1861 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1921 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1981 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2041 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2101 gatccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2161 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2221 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2281 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2341 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2401 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2461 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2521 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2581 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2641 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2701 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2761 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2821 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2881 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2941 gtgccacctg aagatggcag cgcctaaatg tagaatgata aaaggattaa gagattaatt
     3001 tccctaaaaa tgataaaaca agcgttttga aagcgcttgt ttttttggtt tgcagtcaga
     3061 gtagaataga agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     3121 actagcctta ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat tattttataa
     3181 cttttataac aaataatcaa ggagaaattc aaagaaattt atcagccata aaacaatact
     3241 taatactata gaatgataac aaaataaact actttttaaa agaattttgt gttataatct
     3301 atttattatt aagtattggg taatattttt tgaagagata ttttgaaaaa gaaaaattaa
     3361 agcatattaa actaatttcg gaggtcatta aaactattat tgaaatcatc aaactcatta
     3421 tggatttaat ttaaactttt tattttagga ggcaaaaatg gataagaaat actcaatagg
     3481 cttagatatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc
     3541 gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat
     3601 aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc
     3661 tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa
     3721 tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga
     3781 agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta
     3841 tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa
     3901 agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt
     3961 tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt
     4021 ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc
     4081 taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca
     4141 gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt
     4201 gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa
     4261 agatacttac gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga
     4321 tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt
     4381 aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca
     4441 tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa
     4501 agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag
     4561 ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga
     4621 attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg
     4681 ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga
     4741 cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat
     4801 tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa
     4861 gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc
     4921 tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact
     4981 accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa
     5041 atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat
     5101 tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta
     5161 tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa
     5221 tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga
     5281 taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga
     5341 tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat
     5401 gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa
     5461 tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt
     5521 tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat
     5581 tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc
     5641 tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt
     5701 caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca
     5761 gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat
     5821 caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa
     5881 tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt
     5941 agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa
     6001 agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga
     6061 taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa
     6121 cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt
     6181 gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac
     6241 taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa
     6301 acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa
     6361 agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta
     6421 tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt
     6481 tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga
     6541 aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac
     6601 agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga
     6661 aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc
     6721 catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga
     6781 gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc
     6841 aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa
     6901 ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat
     6961 tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa
     7021 ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa
     7081 cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct
     7141 gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag
     7201 tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga
     7261 gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga
     7321 taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa
     7381 tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga
     7441 tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat
     7501 ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga
     7561 ctgaagtata ttttagatga agattatttc ttaataacta aaaatatggt ataatactct
     7621 taataaatgc agtaatacag gggcttttca agactgaagt ctagctgaga caaatagtgc
     7681 gattacgaaa ttttttagac aaaaatagtc tacgaggttt tagagctatg ctgttttgaa
     7741 tggtcccaaa actgagacca gtctcggaag ctcaaaggtc tcgttttaga gctatgctgt
     7801 tttgaatggt cccaaaactt cagcacactg agacttgttg agttccatgt tttagagcta
     7861 tgctgttttg aatggactcc attcaacatt gccgatgata acttgagaaa gagggttaat
     7921 accagcagtc ggataccttc ctattctttc tgttaaagcg ttttcatgtt ataataggca
     7981 aaagaagagt agtgtgatcg tccattcc
//
