LOCUS       sequence-227930-       11666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries bidirectional promoter cassette containing sp44 and p21 
            promoters and apramycin resistance gene flanked by two FRT sites 
            that can be amplified with homology sequence for refactoring..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP03-sp44/p21
FEATURES             Location/Qualifiers
     source          1..11666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    807..840
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             934..1734
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     protein_bind    1738..1771
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             1856..2659
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    2737..3240
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     3282..3300
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3338..3360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3460..3479
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3500..3779
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             3780..4454
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(4694..4715)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      4962..5550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5451..5470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5715..5732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5946..6080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5970..5989)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6024..6043
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6505..6525)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6656..6721
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(7141..7935)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7252..7271)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7862..7881
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(8719..9090)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9123..9232
                     /note="incP origin of transfer"
     CDS             complement(9470..9481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    9552..9651
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             9661..11478
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 cctacaacaa ctaagaaaat ggctatcatg cggaagctgg tgagaagaac agcatcggga
       61 caagggaagg aagaacaaag acaaagaaaa caaaagaaag caattgaaaa caaaacaaaa
      121 caattttcat tccttctctt atcattcctt ttcttttctt ttctctcatt caacgcactc
      181 catcgtatcc gtattcctct tattttttct ctttctctat atccatttct ttctctctag
      241 gtgtgtcctc tctctctctt caatttctct actccgcatt ccaacgcatc cttcccccaa
      301 cctcccattt cctccttacg gcccgatagc gatcgtcttt ccctcgctat cactcgctac
      361 cggcccctcc tctgcaccgt aacctcctac gtatttacca tatcataaag ttttttccga
      421 cgcttatcgc tgaccccctg tcgccctcct attggcttcc ggattatctt cttgtccata
      481 aggtgatcca tgcttcctga agattcccga aatgtgtcca ctttggcggg gaatcattcc
      541 atccacttct ttctctctcg ctttcctcat tcggcgctcc ccttccgcgt ctcattggtc
      601 ttccgctccg tttttgcttt gccgatgtta cttggggaga ggtgcgataa tcctttcgca
      661 aaaactcggt ttgacgcctc ccatggtata aatagtggcc atatgggtga accgatctcc
      721 tcgttagggt cacaccagac tttacaacac cgcacagcat gttgtcaaag cagagacggt
      781 tcgaatgtga acagccactg cagttcgaag ttcctattct ctagaaagta taggaacttc
      841 ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg
      901 caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc
      961 gtgcaatacg aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc
     1021 cccggcggtg tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg
     1081 ccacttggac tgatcgaggc cctgcgtgct gcgctgggtc cgggagggac gctcgtcatg
     1141 ccctcgtggt caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg
     1201 gaccttggag ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat
     1261 ccatttgcct ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc
     1321 ctgccacctc actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta
     1381 cttctcctcg gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca
     1441 aaggttccct atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc
     1501 gtcgattatc tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc
     1561 aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc
     1621 tcccgcgaca ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg
     1681 catccgccag aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagaa
     1741 gttcctattc tctagaaagt ataggaactt caagctttgc tcgagtgtgc gggctctaac
     1801 acgtcctagt atggtaggat gagcaatcta gtcgagcaac ggaggtacgg accatatgtc
     1861 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     1921 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     1981 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     2041 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     2101 caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa
     2161 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     2221 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcggaag aagtaacaaa
     2281 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tagctactgg
     2341 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     2401 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     2461 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     2521 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     2581 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     2641 atgcggccag caaaactaag gtacctgtat ttagaaaaat aaacaaatag gggttccgcg
     2701 cacatttccc cgaaaagtgc cacctgggtc cttttcatca cgtgctataa aaataattat
     2761 aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa
     2821 agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat
     2881 actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt
     2941 ctgtgtagaa gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga
     3001 atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt
     3061 tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct
     3121 tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa
     3181 ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc
     3241 cgtcctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3301 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3421 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     3481 tgagagtgca ccatagatca cgacattact atatatataa tataggaagc atttaataga
     3541 cagcatcgta atatatgtgt actttgcagt tatgacgcca gatggcagta gtggaagata
     3601 ttctttattg aaaaatagct tgtcacctta cgtacaatct tgatccggag cttttctttt
     3661 tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc
     3721 attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta
     3781 tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga
     3841 gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt attatatgtg
     3901 tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa
     3961 aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac
     4021 ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg
     4081 gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg
     4141 tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta
     4201 ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt
     4261 ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta gctggtggac
     4321 tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa
     4381 gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa
     4441 atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc
     4501 gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     4561 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     4621 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     4681 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     4741 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccta actgtcagac
     4801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5101 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5221 tgtcctaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5581 ctggcctttt gctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5641 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5701 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgctga cttccgcgtt
     5761 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     5821 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     5881 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
     5941 cgtcagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     6001 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     6061 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     6121 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     6181 atgatctcta gtcaaggcac tatacatcaa atattcctta ttaacccctt tacaaattaa
     6241 aaagctaaag gtacacaatt tttgagcata gttattaata gcagacactc tatgcctgtg
     6301 tggagtaaga aaaaacagta tgttatgatt ataactgtta tgcctactta taaaggttac
     6361 agaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg tggtgtaaat
     6421 agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac ttagcaattc
     6481 tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag tagaatgttg
     6541 agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa acaggttttc
     6601 ctcattaaag gcattccacc actgctccca ttcatcagtt tccataggtt ggaatctaaa
     6661 atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat tttatattta
     6721 ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca cagaagtaag
     6781 gttccttcac aaagatccgg accaaagcgg ccatcgtgcc tccccactcc tgcagttcgg
     6841 gggcatggat gcgcggatag ccgctgctgg tttcctggat gccgacggat ttgcactgcc
     6901 ggtagaactc cgcgaggtcg tccagcctca ggcagcagct gaaccaactc gcgaggggat
     6961 cgagcccggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     7021 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     7081 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     7141 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     7201 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     7261 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     7321 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     7381 gacgagatcc tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc
     7441 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     7501 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     7561 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     7621 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     7681 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     7741 cgctgcctcg tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     7801 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     7861 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     7921 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
     7981 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     8041 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     8101 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     8161 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     8221 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     8281 gcgtgagagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg
     8341 ctggggcaac acgtggagcg gatcggggat tgtctttctt cagctcgctg atgatatgct
     8401 gacgctcaat gccgtttggc ctccgactaa cgaaaatccc gcatttggac ggctgatccg
     8461 attggcacgg cggacggcga atggcggagc agacgctcgt ccgggggcaa tgagatatga
     8521 aaaagcctga actcaccgcg acgtatcggg ccctggccag ctagctagag tcgacctgca
     8581 ggtccccggg gatcggtctt gccttgctcg tcggtgatgt acttcaccag ctccgcgaag
     8641 tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg
     8701 ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt
     8761 gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc
     8821 gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc
     8881 caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat
     8941 tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc
     9001 cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc
     9061 cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
     9121 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
     9181 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
     9241 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
     9301 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
     9361 ggaaaagatc cgtcgacctg caggcatgca agctctagcg attccagacg tcccgaaggc
     9421 gtggcgcggc ttccccgtgc cggagcaatc gccctgggtg ggttacacga cgcccctcta
     9481 tggcccgtac tgacggacac accgaagccc cggcggcaac cctcagcgga tgccccgggg
     9541 cttcacgttt tcccaggtca gaagcggttt tcgggagtag tgccccaact ggggtaacct
     9601 ttgagttctc tcagttgggg gcgtagggtc gccgacatga cacaaggggt tgtgaccggg
     9661 gtggacacgt acgcgggtgc ttacgaccgt cagtcgcgcg agcgcgaaaa ttcgagcgca
     9721 gcaagcccag cgacacagcg tagcgccaac gaagacaagg cggccgacct tcagcgcgaa
     9781 gtcgagcgcg acgggggccg gttcaggttc gtcgggcatt tcagcgaagc gccgggcacg
     9841 tcggcgttcg ggacggcgga gcgcccggag ttcgaacgca tcctgaacga atgccgcgcc
     9901 gggcggctca acatgatcat tgtctatgac gtgtcgcgct tctcgcgcct gaaggtcatg
     9961 gacgcgattc cgattgtctc ggaattgctc gccctgggcg tgacgattgt ttccactcag
    10021 gaaggcgtct tccggcaggg aaacgtcatg gacctgattc acctgattat gcggctcgac
    10081 gcgtcgcaca aagaatcttc gctgaagtcg gcgaagattc tcgacacgaa gaaccttcag
    10141 cgcgaattgg gcgggtacgt cggcgggaag gcgccttacg gcttcgagct tgtttcggag
    10201 acgaaggaga tcacgcgcaa cggccgaatg gtcaatgtcg tcatcaacaa gcttgcgcac
    10261 tcgaccactc cccttaccgg acccttcgag ttcgagcccg acgtaatccg gtggtggtgg
    10321 cgtgagatca agacgcacaa acaccttccc ttcaagccgg gcagtcaagc cgccattcac
    10381 ccgggcagca tcacggggct ttgtaagcgc atggacgctg acgccgtgcc gacccggggc
    10441 gagacgattg ggaagaagac cgcttcaagc gcctgggacc cggcaaccgt tatgcgaatc
    10501 cttcgggccc cgcgtattgc gggcttcgcc gctgaggtga tctacaagaa gaagccggac
    10561 ggcacgccga ccacgaagat tgagggttac cgcattcagc gcgacccgat cacgctccgg
    10621 ccggtcgagc ttgattgcgg accgatcatc gagcccgctg agtggtatga gcttcaggcg
    10681 tggttggacg gcagggggcg cggcaagggg ctttcccggg ggcaagccat tctgtccgcc
    10741 atggacaagc tgtactgcga gtgtggcgcc gtcatgactt cgaagcgcgg ggaagaatcg
    10801 atcaaggact cttaccgctg ccgtcgccgg aaggtggtcg acccgtccgc acctgggcag
    10861 cacgaaggca cgtgcaacgt cagcatggcg gcactcgaca agttcgttgc ggaacgcatc
    10921 ttcaacaaga tcaggcacgc cgaaggcgac gaagagacgt tggcgcttct gtgggaagcc
    10981 gcccgacgct tcggcaagct cactgaggcg cctgagaaga gcggcgaacg ggcgaacctt
    11041 gttgcggagc gcgccgacgc cctgaacgcc cttgaagagc tgtacgaaga ccgcgcggca
    11101 ggcgcgtacg acggacccgt tggcaggaag cacttccgga agcaacaggc agcgctgacg
    11161 ctccggcagc aaggggcgga agagcggctt gccgaacttg aagccgccga agccccgaag
    11221 cttccccttg accaatggtt ccccgaagac gccgacgctg acccgaccgg ccctaagtcg
    11281 tggtgggggc gcgcgtcagt agacgacaag cgcgtgttcg tcgggctctt cgtagacaag
    11341 atcgttgtca cgaagtcgac tacgggcagg gggcagggaa cgcccatcga gaagcgcgct
    11401 tcgatcacgt gggcgaagcc gccgaccgac gacgacgaag acgacgccca ggacggcacg
    11461 gaagacgtag cggcgtagcg agacacccgg gaagcctgat ctacgtctgt cgagaagttt
    11521 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcgcaggg cgaagaatct
    11581 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
    11641 gatggtttct acaaagatcg actagt
//
