LOCUS       sequence-227931-       12581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries promoter cassette containing act1p promoter and apramycin 
            resistance gene that can be amplified with homology sequence for 
            refactoring..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 12581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP03-act1p
FEATURES             Location/Qualifiers
     source          1..12581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             798..1598
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             2774..3574
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="SKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLE
                     LVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQY
                     SAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGEY
                     TKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDV
                     VSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    3652..4155
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     4197..4215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4253..4275)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4375..4394
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4415..4694
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             4695..5369
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(5609..5630)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5877..6465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6366..6385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6630..6647
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6861..6995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6885..6904)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6939..6958
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7420..7440)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7571..7636
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(8056..8850)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8167..8186)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8777..8796
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(9634..10005)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10038..10147
                     /note="incP origin of transfer"
     CDS             complement(10385..10396)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    10467..10566
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             10576..12393
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 cctacaacaa ctaagaaaat ggctatcatg cggaagctgg tgagaagaac agcatcggga
       61 caagggaagg aagaacaaag acaaagaaaa caaaagaaag caattgaaaa caaaacaaaa
      121 caattttcat tccttctctt atcattcctt ttcttttctt ttctctcatt caacgcactc
      181 catcgtatcc gtattcctct tattttttct ctttctctat atccatttct ttctctctag
      241 gtgtgtcctc tctctctctt caatttctct actccgcatt ccaacgcatc cttcccccaa
      301 cctcccattt cctccttacg gcccgatagc gatcgtcttt ccctcgctat cactcgctac
      361 cggcccctcc tctgcaccgt aacctcctac gtatttacca tatcataaag ttttttccga
      421 cgcttatcgc tgaccccctg tcgccctcct attggcttcc ggattatctt cttgtccata
      481 aggtgatcca tgcttcctga agattcccga aatgtgtcca ctttggcggg gaatcattcc
      541 atccacttct ttctctctcg ctttcctcat tcggcgctcc ccttccgcgt ctcattggtc
      601 ttccgctccg tttttgcttt gccgatgtta cttggggaga ggtgcgataa tcctttcgca
      661 aaaactcggt ttgacgcctc ccatggtata aatagtggct cgagggttca tgtgcagctc
      721 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
      781 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
      841 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
      901 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
      961 aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     1021 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     1081 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     1141 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca cctcactcgc
     1201 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     1261 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     1321 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     1381 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     1441 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     1501 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     1561 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg acatatgcca ctgcctctcg
     1621 gtaaaatcca gcaaaaatta atcagtgcag ctcgctgcac tgattaattt ttgatcaata
     1681 ggagatcgct tgtgacggca agcacattga aatctgttga gtaggcctgt tattgtcgcc
     1741 cccaggagac ggagaatctc gacgggggcg cagatgagat tcaacttatt gggacgtgtc
     1801 catgtaatca ccgatgcggg atgtgtaatt ccgcttaaat cctcgaaggc gacccagctc
     1861 ctggtgctgc tgctcctcag gcggcacgag gtggtgggat cgggggtgct catcgaggag
     1921 ttgtgggcgg accacccgcc ccgcagcgcc atgacgacgc tgcagacgta cgtgtaccac
     1981 acccgccggc tgctggggga gcaccgggtg acgagcgacg accgggaatt ggtcctgacc
     2041 cagccgcccg gctacttcgc cctgatcgac gaggacgaac tcgacgtcgc ggtcgccgag
     2101 cgtctgatcc gcaccggcgg ccggctgctc gaggagaacc ggctcgagga ggcgctcgcc
     2161 tcgttggacg cgggactgga tctctggcga ggcccggcgc tgtccaccgt accgtgcggc
     2221 cgggtgctcg aaagcaatat cgcgcacctg gaagagctgc ggctttttgg aatgcagctc
     2281 cgtatcgacg cgaattggcg gctgggcaga atagggccga tgattccgga actccggtcc
     2341 ctggtaattt cgcatccgct gaacgagacc ctgcacgcca aactgatggg cgcgctctgt
     2401 cagatgggca ggcgcgccga ggcgctggaa tcgtatcgga atctccggcg gatactgtcc
     2461 gacgaactgg gggtggatcc gacgccggaa atccagcgta tgcacatgga aattctcaac
     2521 ggtgagaagg tgctcgtgta gcaccggtcc gtgaacgcgg tggagcccta tgtctcttaa
     2581 gtgttcccct ccctgcctcg tggtccctca cgcgctcagc tttgggcgcc cggctcgagc
     2641 ggcggtcgaa gggagatggg gtgccgctgg acgcggcgcc ggtggatccg gcatcgaggg
     2701 gtcccgtatc ggccttcgag cctccttcga gccacggggc cgacgatgac gacgaccacc
     2761 ggacgaacgc atctcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt
     2821 tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga
     2881 tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt
     2941 actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct
     3001 aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat
     3061 tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga
     3121 cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc
     3181 ggaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg
     3241 ctccctagct actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga
     3301 ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg
     3361 gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta
     3421 tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact
     3481 atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga
     3541 agcatatttg agaagatgcg gccagcaaaa ctaaggtacc tgtatttaga aaaataaaca
     3601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc
     3661 tataaaaata attataattt aaatttttta atataaatat ataaattaaa aatagaaagt
     3721 aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg
     3781 ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga
     3841 attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta
     3901 cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat
     3961 gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc
     4021 gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa
     4081 taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt
     4141 tacaggcaag cgatccgtcc taagaaacca ttattatcat gacattaacc tataaaaata
     4201 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4261 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4321 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     4381 cagagcagat tgtactgaga gtgcaccata gatcacgaca ttactatata tataatatag
     4441 gaagcattta atagacagca tcgtaatata tgtgtacttt gcagttatga cgccagatgg
     4501 cagtagtgga agatattctt tattgaaaaa tagcttgtca ccttacgtac aatcttgatc
     4561 cggagctttt ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg
     4621 gccaagaggg agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa
     4681 aggcagcttg gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag
     4741 tttgcggctt gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc
     4801 tgggtattat atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa
     4861 tttcaagtct tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg
     4921 tgtttcgtaa tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata
     4981 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
     5041 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
     5101 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     5161 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta
     5221 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     5281 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     5341 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     5401 gtttgtgcac ttgccgatct atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5461 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     5521 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     5581 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     5641 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     5701 acctaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5761 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5821 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5881 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5941 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6001 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6061 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6121 agtggcgata agtcgtgtcc taccgggttg gactcaagac gatagttacc ggataaggcg
     6181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6481 gcctttttac ggttcctggc cttttgctgg ccttttgctg gccttttgct cacatgttct
     6541 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6661 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     6721 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     6781 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     6841 acgatcatgc gcacccgtca gatccagaca tgataagata cattgatgag tttggacaaa
     6901 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6961 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     7021 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     7081 gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt ccttattaac
     7141 ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat taatagcaga
     7201 cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac tgttatgcct
     7261 acttataaag gttacagaat atttttccat aattttcttg tatagcagtg cagctttttc
     7321 ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa
     7381 aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg
     7441 aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga
     7501 gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat cagtttccat
     7561 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
     7621 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
     7681 caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc
     7741 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
     7801 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc
     7861 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct
     7921 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     7981 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     8041 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     8101 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     8161 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     8221 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     8281 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa
     8341 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     8401 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     8461 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     8521 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     8581 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     8641 cagccacgat agccgcgctg cctcgtcttg gagttcattc agggcaccgg acaggtcggt
     8701 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     8761 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     8821 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     8881 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     8941 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     9001 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     9061 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     9121 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     9181 tgagtgcttg cggcagcgtg agagctcatg agcggagaac gagatgacgt tggaggggca
     9241 aggtcgcgct gattgctggg gcaacacgtg gagcggatcg gggattgtct ttcttcagct
     9301 cgctgatgat atgctgacgc tcaatgccgt ttggcctccg actaacgaaa atcccgcatt
     9361 tggacggctg atccgattgg cacggcggac ggcgaatggc ggagcagacg ctcgtccggg
     9421 ggcaatgaga tatgaaaaag cctgaactca ccgcgacgta tcgggccctg gccagctagc
     9481 tagagtcgac ctgcaggtcc ccggggatcg gtcttgcctt gctcgtcggt gatgtacttc
     9541 accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg
     9601 cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac
     9661 gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag
     9721 gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag
     9781 gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc
     9841 cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat
     9901 gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt
     9961 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
    10021 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
    10081 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
    10141 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
    10201 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
    10261 gcagggttat gcagcggaaa agatccgtcg acctgcaggc atgcaagctc tagcgattcc
    10321 agacgtcccg aaggcgtggc gcggcttccc cgtgccggag caatcgccct gggtgggtta
    10381 cacgacgccc ctctatggcc cgtactgacg gacacaccga agccccggcg gcaaccctca
    10441 gcggatgccc cggggcttca cgttttccca ggtcagaagc ggttttcggg agtagtgccc
    10501 caactggggt aacctttgag ttctctcagt tgggggcgta gggtcgccga catgacacaa
    10561 ggggttgtga ccggggtgga cacgtacgcg ggtgcttacg accgtcagtc gcgcgagcgc
    10621 gaaaattcga gcgcagcaag cccagcgaca cagcgtagcg ccaacgaaga caaggcggcc
    10681 gaccttcagc gcgaagtcga gcgcgacggg ggccggttca ggttcgtcgg gcatttcagc
    10741 gaagcgccgg gcacgtcggc gttcgggacg gcggagcgcc cggagttcga acgcatcctg
    10801 aacgaatgcc gcgccgggcg gctcaacatg atcattgtct atgacgtgtc gcgcttctcg
    10861 cgcctgaagg tcatggacgc gattccgatt gtctcggaat tgctcgccct gggcgtgacg
    10921 attgtttcca ctcaggaagg cgtcttccgg cagggaaacg tcatggacct gattcacctg
    10981 attatgcggc tcgacgcgtc gcacaaagaa tcttcgctga agtcggcgaa gattctcgac
    11041 acgaagaacc ttcagcgcga attgggcggg tacgtcggcg ggaaggcgcc ttacggcttc
    11101 gagcttgttt cggagacgaa ggagatcacg cgcaacggcc gaatggtcaa tgtcgtcatc
    11161 aacaagcttg cgcactcgac cactcccctt accggaccct tcgagttcga gcccgacgta
    11221 atccggtggt ggtggcgtga gatcaagacg cacaaacacc ttcccttcaa gccgggcagt
    11281 caagccgcca ttcacccggg cagcatcacg gggctttgta agcgcatgga cgctgacgcc
    11341 gtgccgaccc ggggcgagac gattgggaag aagaccgctt caagcgcctg ggacccggca
    11401 accgttatgc gaatccttcg ggccccgcgt attgcgggct tcgccgctga ggtgatctac
    11461 aagaagaagc cggacggcac gccgaccacg aagattgagg gttaccgcat tcagcgcgac
    11521 ccgatcacgc tccggccggt cgagcttgat tgcggaccga tcatcgagcc cgctgagtgg
    11581 tatgagcttc aggcgtggtt ggacggcagg gggcgcggca aggggctttc ccgggggcaa
    11641 gccattctgt ccgccatgga caagctgtac tgcgagtgtg gcgccgtcat gacttcgaag
    11701 cgcggggaag aatcgatcaa ggactcttac cgctgccgtc gccggaaggt ggtcgacccg
    11761 tccgcacctg ggcagcacga aggcacgtgc aacgtcagca tggcggcact cgacaagttc
    11821 gttgcggaac gcatcttcaa caagatcagg cacgccgaag gcgacgaaga gacgttggcg
    11881 cttctgtggg aagccgcccg acgcttcggc aagctcactg aggcgcctga gaagagcggc
    11941 gaacgggcga accttgttgc ggagcgcgcc gacgccctga acgcccttga agagctgtac
    12001 gaagaccgcg cggcaggcgc gtacgacgga cccgttggca ggaagcactt ccggaagcaa
    12061 caggcagcgc tgacgctccg gcagcaaggg gcggaagagc ggcttgccga acttgaagcc
    12121 gccgaagccc cgaagcttcc ccttgaccaa tggttccccg aagacgccga cgctgacccg
    12181 accggcccta agtcgtggtg ggggcgcgcg tcagtagacg acaagcgcgt gttcgtcggg
    12241 ctcttcgtag acaagatcgt tgtcacgaag tcgactacgg gcagggggca gggaacgccc
    12301 atcgagaagc gcgcttcgat cacgtgggcg aagccgccga ccgacgacga cgaagacgac
    12361 gcccaggacg gcacggaaga cgtagcggcg tagcgagaca cccgggaagc ctgatctacg
    12421 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
    12481 cagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
    12541 gtaaatagct gcgccgatgg tttctacaaa gatcgactag t
//
