LOCUS       sequence-227932-       11601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries promoter cassette containing ermE*p promoter and ampicillin 
            resistance gene that can be amplified with homology sequence for 
            refactoring..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya
            H, Moore BS
  TITLE     Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster 
            Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
  JOURNAL   Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004.
  PUBMED    30853419
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Chem 
            Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 
            10.1016/j.chembiol.2019.02.004."
COMMENT     Sequence Label: pCAP03-ermE*p
FEATURES             Location/Qualifiers
     source          1..11601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(778..846,847..1638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(996..1015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             1794..2594
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="SKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLE
                     LVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQY
                     SAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGEY
                     TKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDV
                     VSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    2672..3175
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     3217..3235
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3273..3295)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3395..3414
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3435..3714
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             3715..4389
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(4629..4650)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      4897..5485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5386..5405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5650..5667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5881..6015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5905..5924)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5959..5978
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6440..6460)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6591..6656
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(7076..7870)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7187..7206)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7797..7816
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(8654..9025)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9058..9167
                     /note="incP origin of transfer"
     CDS             complement(9405..9416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    9487..9586
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             9596..11413
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RAPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
ORIGIN
        1 cctacaacaa ctaagaaaat ggctatcatg cggaagctgg tgagaagaac agcatcggga
       61 caagggaagg aagaacaaag acaaagaaaa caaaagaaag caattgaaaa caaaacaaaa
      121 caattttcat tccttctctt atcattcctt ttcttttctt ttctctcatt caacgcactc
      181 catcgtatcc gtattcctct tattttttct ctttctctat atccatttct ttctctctag
      241 gtgtgtcctc tctctctctt caatttctct actccgcatt ccaacgcatc cttcccccaa
      301 cctcccattt cctccttacg gcccgatagc gatcgtcttt ccctcgctat cactcgctac
      361 cggcccctcc tctgcaccgt aacctcctac gtatttacca tatcataaag ttttttccga
      421 cgcttatcgc tgaccccctg tcgccctcct attggcttcc ggattatctt cttgtccata
      481 aggtgatcca tgcttcctga agattcccga aatgtgtcca ctttggcggg gaatcattcc
      541 atccacttct ttctctctcg ctttcctcat tcggcgctcc ccttccgcgt ctcattggtc
      601 ttccgctccg tttttgcttt gccgatgtta cttggggaga ggtgcgataa tcctttcgca
      661 aaaactcggt ttgacgcctc ccatggtata aatagtggct cgagcattca aatatgtatc
      721 cgctcatgag acaataaccc tgataaatgc ttcaatcatg attgaaaaag gaagagtatg
      781 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      841 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      901 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      961 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1021 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1081 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1141 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1201 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1261 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1321 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1381 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1441 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1501 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1561 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1621 ctgattaagc attggtaaca tatgtggtgc acgcggtcga tcttgacggc tggcgagagg
     1681 tgcggggagg atctgaccga cgcggtccac acgtggcacc gcgatgctgt tgtgggcaca
     1741 atcgtgccgg ttggtaggat ccagcggtcc aacgcacccc aggaggtccc cattcgaaag
     1801 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     1861 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     1921 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     1981 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     2041 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     2101 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     2161 tggtgggccc aggtattgtt agcggtttga agcaggcggc ggaagaagta acaaaggaac
     2221 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctagct actggagaat
     2281 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     2341 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     2401 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     2461 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     2521 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     2581 gccagcaaaa ctaaggtacc tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2641 ttccccgaaa agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt
     2701 aaatttttta atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa
     2761 aaatagtttt tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac
     2821 cttttatctt gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg
     2881 tagaagacca cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta
     2941 tatctattta atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg
     3001 aaatttttta aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt
     3061 atttactttc taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc
     3121 aaattattcc atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc
     3181 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     3241 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3361 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     3421 gtgcaccata gatcacgaca ttactatata tataatatag gaagcattta atagacagca
     3481 tcgtaatata tgtgtacttt gcagttatga cgccagatgg cagtagtgga agatattctt
     3541 tattgaaaaa tagcttgtca ccttacgtac aatcttgatc cggagctttt ctttttttgc
     3601 cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg
     3661 tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct
     3721 gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca
     3781 gaggccgcag aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc
     3841 aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca
     3901 tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag
     3961 gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat
     4021 gagtcgtggc aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt
     4081 ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc
     4141 ttgtttgatt cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac
     4201 tgggttggaa ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg
     4261 ccagaaaatg ttggtgatgc gcttagatta aatggcgtta ttggtgttga tgtaagcgga
     4321 ggtgtggaga caaatggtgt aaaagactct aacaaaatag caaatttcgt caaaaatgct
     4381 aagaaatagg ttattactga gtagtattta tttaagtatt gtttgtgcac ttgccgatct
     4441 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     4501 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4561 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4621 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4681 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga acctaactgt cagaccaagt
     4741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5041 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtcc
     5161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5521 cttttgctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc gcgtttccag
     5701 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     5761 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     5821 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtca
     5881 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5941 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6001 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6061 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     6121 ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa attaaaaagc
     6181 taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc ctgtgtggag
     6241 taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag gttacagaat
     6301 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
     6361 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
     6421 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
     6481 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
     6541 taaaggcatt ccaccactgc tcccattcat cagtttccat aggttggaat ctaaaataca
     6601 caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat atttacctta
     6661 gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa gtaaggttcc
     6721 ttcacaaaga tccggaccaa agcggccatc gtgcctcccc actcctgcag ttcgggggca
     6781 tggatgcgcg gatagccgct gctggtttcc tggatgccga cggatttgca ctgccggtag
     6841 aactccgcga ggtcgtccag cctcaggcag cagctgaacc aactcgcgag gggatcgagc
     6901 ccggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     6961 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     7021 cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga
     7081 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     7141 aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag
     7201 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     7261 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga
     7321 gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     7381 cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     7441 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     7501 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     7561 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     7621 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     7681 cctcgtcttg gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     7741 gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc
     7801 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     7861 gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc
     7921 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     7981 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     8041 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     8101 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     8161 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg
     8221 agagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg
     8281 gcaacacgtg gagcggatcg gggattgtct ttcttcagct cgctgatgat atgctgacgc
     8341 tcaatgccgt ttggcctccg actaacgaaa atcccgcatt tggacggctg atccgattgg
     8401 cacggcggac ggcgaatggc ggagcagacg ctcgtccggg ggcaatgaga tatgaaaaag
     8461 cctgaactca ccgcgacgta tcgggccctg gccagctagc tagagtcgac ctgcaggtcc
     8521 ccggggatcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct
     8581 cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta
     8641 gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa
     8701 gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc
     8761 gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt
     8821 cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt
     8881 agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt
     8941 cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc
     9001 tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg
     9061 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
     9121 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
     9181 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
     9241 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
     9301 agatccgtcg acctgcaggc atgcaagctc tagcgattcc agacgtcccg aaggcgtggc
     9361 gcggcttccc cgtgccggag caatcgccct gggtgggtta cacgacgccc ctctatggcc
     9421 cgtactgacg gacacaccga agccccggcg gcaaccctca gcggatgccc cggggcttca
     9481 cgttttccca ggtcagaagc ggttttcggg agtagtgccc caactggggt aacctttgag
     9541 ttctctcagt tgggggcgta gggtcgccga catgacacaa ggggttgtga ccggggtgga
     9601 cacgtacgcg ggtgcttacg accgtcagtc gcgcgagcgc gaaaattcga gcgcagcaag
     9661 cccagcgaca cagcgtagcg ccaacgaaga caaggcggcc gaccttcagc gcgaagtcga
     9721 gcgcgacggg ggccggttca ggttcgtcgg gcatttcagc gaagcgccgg gcacgtcggc
     9781 gttcgggacg gcggagcgcc cggagttcga acgcatcctg aacgaatgcc gcgccgggcg
     9841 gctcaacatg atcattgtct atgacgtgtc gcgcttctcg cgcctgaagg tcatggacgc
     9901 gattccgatt gtctcggaat tgctcgccct gggcgtgacg attgtttcca ctcaggaagg
     9961 cgtcttccgg cagggaaacg tcatggacct gattcacctg attatgcggc tcgacgcgtc
    10021 gcacaaagaa tcttcgctga agtcggcgaa gattctcgac acgaagaacc ttcagcgcga
    10081 attgggcggg tacgtcggcg ggaaggcgcc ttacggcttc gagcttgttt cggagacgaa
    10141 ggagatcacg cgcaacggcc gaatggtcaa tgtcgtcatc aacaagcttg cgcactcgac
    10201 cactcccctt accggaccct tcgagttcga gcccgacgta atccggtggt ggtggcgtga
    10261 gatcaagacg cacaaacacc ttcccttcaa gccgggcagt caagccgcca ttcacccggg
    10321 cagcatcacg gggctttgta agcgcatgga cgctgacgcc gtgccgaccc ggggcgagac
    10381 gattgggaag aagaccgctt caagcgcctg ggacccggca accgttatgc gaatccttcg
    10441 ggccccgcgt attgcgggct tcgccgctga ggtgatctac aagaagaagc cggacggcac
    10501 gccgaccacg aagattgagg gttaccgcat tcagcgcgac ccgatcacgc tccggccggt
    10561 cgagcttgat tgcggaccga tcatcgagcc cgctgagtgg tatgagcttc aggcgtggtt
    10621 ggacggcagg gggcgcggca aggggctttc ccgggggcaa gccattctgt ccgccatgga
    10681 caagctgtac tgcgagtgtg gcgccgtcat gacttcgaag cgcggggaag aatcgatcaa
    10741 ggactcttac cgctgccgtc gccggaaggt ggtcgacccg tccgcacctg ggcagcacga
    10801 aggcacgtgc aacgtcagca tggcggcact cgacaagttc gttgcggaac gcatcttcaa
    10861 caagatcagg cacgccgaag gcgacgaaga gacgttggcg cttctgtggg aagccgcccg
    10921 acgcttcggc aagctcactg aggcgcctga gaagagcggc gaacgggcga accttgttgc
    10981 ggagcgcgcc gacgccctga acgcccttga agagctgtac gaagaccgcg cggcaggcgc
    11041 gtacgacgga cccgttggca ggaagcactt ccggaagcaa caggcagcgc tgacgctccg
    11101 gcagcaaggg gcggaagagc ggcttgccga acttgaagcc gccgaagccc cgaagcttcc
    11161 ccttgaccaa tggttccccg aagacgccga cgctgacccg accggcccta agtcgtggtg
    11221 ggggcgcgcg tcagtagacg acaagcgcgt gttcgtcggg ctcttcgtag acaagatcgt
    11281 tgtcacgaag tcgactacgg gcagggggca gggaacgccc atcgagaagc gcgcttcgat
    11341 cacgtgggcg aagccgccga ccgacgacga cgaagacgac gcccaggacg gcacggaaga
    11401 cgtagcggcg tagcgagaca cccgggaagc ctgatctacg tctgtcgaga agtttctgat
    11461 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg cagggcgaag aatctcgtgc
    11521 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
    11581 tttctacaaa gatcgactag t
//
