LOCUS       sequence-227934-        4563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF285 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF285
FEATURES             Location/Qualifiers
     source          1..4563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1874..1973)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1990..2009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1991..2009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2014..2030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2014..2030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2461..3252
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3345..3933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3834..3853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4087..4104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4263..4290)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4382..4468)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4517..4539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4531..4548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4532..4548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgattaa gttccaggaa
      121 agggtgacat tcaaggatgt ggctgttgtc ttcaccaagg aagagctggc actattggat
      181 aaagcccaga taaacctgta ccaagatgtg atgctggaaa acttcaggaa cctcatgtta
      241 gtgagagacg ggattaaaaa caacattttg aatcttcagg caaaggggtt aagttacctt
      301 tcgcaagaag tgcttcattg ctggcagatt tggaaacaaa ggatccggga tttaactgtg
      361 agtcaggatt atatcgtgaa ccttcaagaa gagtgttccc cacatttaga agatgtttcc
      421 ctcagtgaag agtgggcagg catttctctt cagatttctg aaaatgaaaa ctatgtagta
      481 aatgccatta tcaaaaatca agatatcaca gcatggcaaa gcctgacaca ggttcttacc
      541 ccagaatcgt ggaggaaagc caacataatg accgagcccc agaactctca gggaagatat
      601 aagggaattt acatggaaga gaaattgtac agacgtgctc agcatgatga cagcctcagt
      661 tggacctcat gtgatcatca tgagtcccaa gaatgtaaag gagaggaccc tggtagacat
      721 cccagctgtg ggaaaaactt gggtatgaaa tcaacggttg aaaaacgtaa tgcggcccat
      781 gtattaccac agcctttccc atgtaataac tgtggggtgg cctttgcaga tgatacagat
      841 cctcatgtcc atcacagcac tcacctagga gaaaaatctt ataaatgtga ccagtatgga
      901 aagaacttta gtcagagcca agatcttatc gttcattgta aaactcactc tggcaagact
      961 ccctatgaat tccacgaatg gcctatgggc tgcaaacaga gctcagacct tcccagatat
     1021 cagaaagtct cctcaggaga caaaccctac aaatgtaaag aatgtggcaa gggcttcagg
     1081 cgcagctctt cccttcacaa ccatcatcga gtccacacag gggagatgcc ctacaaatgc
     1141 gatgaatgtg ggaaagggtt tggatttagg tcacttcttt gtattcatca gggagtacac
     1201 acagggaaaa agccctataa atgtgaagag tgtgggaagg gctttgatca gagctccaac
     1261 cttcttgtcc atcagagagt ccacactgga gagaagccct acaaatgcag tgagtgtggc
     1321 aagtgcttta gttcaagctc cgttcttcaa gtccactgga ggtttcacac aggggagaaa
     1381 ccatataggt gtggtgagtg tggaaagggc ttcagccaat gtacacacct tcacattcac
     1441 cagagagtcc acacagggga gaaaccatac aaatgcaatg tgtgtggaaa ggattttgcg
     1501 tatagctctg ttcttcacac tcatcagaga gttcacactg gagaaaaacc atataaatgt
     1561 gaagtgtgtg gaaagtgctt cagttacagt tcatattttc acttacatca aagagatcac
     1621 atcagagaga aaccatataa atgtgatgag tgtggtaaag gcttcagccg gaattcagat
     1681 cttaatgttc acctcagagt ccacacagga gagaggccct ataagtgtaa ggcatgtggt
     1741 aagggcttca gtcgtaattc atacctcctt gcccatcaga gagtgcatat agatgagaca
     1801 cagtacacac actgtgagcg tggaaaggac cttctgactc atcaaagact acatgagcag
     1861 agagaaacat tattgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
     1921 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1981 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     2041 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     2101 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     2161 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     2221 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2281 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     2341 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     2401 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     2461 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     2521 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     2581 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     2641 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     2701 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     2761 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     2821 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     2881 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     2941 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     3001 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     3061 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     3121 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     3181 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     3241 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3301 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3481 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3541 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3601 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3661 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3721 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3781 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3841 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3901 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3961 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4021 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4081 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4141 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4201 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     4261 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4321 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4381 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4441 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4501 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4561 ccc
//
