LOCUS       sequence-227941-       10230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Mfsd10 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Mfsd10
FEATURES             Location/Qualifiers
     source          1..10230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4977..5526
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5121..5138)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5348..5367
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5529..6128
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5529..5548)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6025..6045
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6152..6740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6205..6225)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6623..6634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6948..7581
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7710..7726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7710..7726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7723..7745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7734..7750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7758..7764,7765..7782,7783..7788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7803..7824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7941..7958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8112..8697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8192..8211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8868..9659,9660..9728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9491..9510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9729..9833)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9881..10015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9918..9937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9972..9991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gggctgggca ggggatgcgg
     3481 gctgtacgcc tcgtccgcct atacggcctc ggcccgcgtc cgagcgtcgc gtgatcatcg
     3541 tgctcttcct cggcctgcta ctcgacctcc tggcgttcac gctgttgctg cctctgctgc
     3601 ctgggctgct ggagagacac ggccgcgaac aagaccccct ctatggctcc tggcagcgtg
     3661 gggtagactg gtttgcttca gccatcggga tgccagcgga aaagaggtac aacagtgtcc
     3721 tgttcggagg tctcatcggc tcggccttct ccctcctgca gttcttctcc gcaccactca
     3781 caggagctgc ctctgattac ctggggaggc gcccagtgat gatgttgtcc ctgacaggtt
     3841 tggccatctc ctatgcagtg tgggctacct ctcggagctt taaggccttc ttggcctcca
     3901 gggtgatcgg aggcatcagc aaggggaacg ttaacctctc cacagccatt gtcgctgacc
     3961 tgggctcacc tccaacccgc agccaaggca tggcagtcat cggggtagcc ttctccttgg
     4021 cctttactct gggtcccatg ctgggagcat tcctgtctgt ggaaatggtg ccctggatca
     4081 gcctactttt tgccatctcc gacatgttgt tcatcttctg tttcttgcca gagacactgc
     4141 cccaggaaaa gcgggcatct tctgtcaccc tgggcttcca cactgctgcc cacctgctca
     4201 gccccctggc cctgcttcgc tttgcggctg tcactcacag tcaggacccg cctgctgagc
     4261 acagactcag aaacttacgc cgtttgggtc ttgtctactt cctctacctc ttcctgttct
     4321 caggcctgga gtacacactc agcttcctgg cacatcagcg cttccagttc agcagcctgc
     4381 agcagggcaa gatgtttttc ttcatcggcc tcaccatggc caccatacag ggcacctacg
     4441 cccggcggat cagccctggc aaggaagctg cagctgtgac gagggcaatg ctgctgctag
     4501 taccagcctt cctcttaatt ggctgggcac actcgctgcc tacgttgggc ttgggactga
     4561 tgctctactc ctttgctgct gctgttgtgg tgcctggtct gtccaccatg gtctccagtt
     4621 atggttctcc agggcaaaaa ggcacaatca tgggcatact gcggagccta ggcgccctag
     4681 gcagggcgtt ggggccggtg gtggctgcct cagtgtactg gctgactggg gcccaggtct
     4741 gcttcactgt gtgctcggcc ctctttctgc tccccttcct actcctgtgg aagctgaagc
     4801 atccagcaga gacttccaag gaggagtaat gggccgcgac gatgtttaaa cttcgaaacg
     4861 cgtgaattcc tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact
     4921 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     4981 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5041 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5101 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5161 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5221 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5281 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5341 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5401 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5461 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     5521 ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5581 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5641 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5701 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5761 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5821 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5881 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5941 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6001 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6061 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6121 gtgcctgacc cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6181 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6241 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6301 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6361 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6421 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6481 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6541 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6601 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6661 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6721 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     6781 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6841 gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta
     6901 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     6961 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7021 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     7081 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     7141 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     7201 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     7261 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     7321 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     7381 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7561 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7621 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
     7681 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7741 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7801 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7861 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8041 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8641 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9841 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
     9901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10021 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    10081 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10141 aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat
    10201 ttgttaaata tgtactacaa acttagtagt
//
