LOCUS       sequence-227943-       10371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Vrk2 in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-V5-Vrk2
FEATURES             Location/Qualifiers
     source          1..10371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5118..5667
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5262..5279)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5489..5508
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5670..6269
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5670..5689)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6166..6186
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6293..6881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6346..6366)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6764..6775)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7089..7722
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7851..7867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7851..7867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7864..7886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7899..7905,7906..7923,7924..7929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7944..7965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8082..8099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8253..8838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8333..8352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9009..9800,9801..9869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9632..9651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9870..9974)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10022..10156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10059..10078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10113..10132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggcaccaaga agaaaagaga
     3481 aatataagct tccggttcca ctcccagaag gcaaaattct ggatgatatg gaaggaaacc
     3541 ggtgggcact gggcaagatg atcggctctg gagggtttgg actgatatac ttagctttcc
     3601 ccacaaataa accaaacaaa gatgcaagac atgtcataaa gctggagtat caagaaaatg
     3661 gcccattatt ttcagagctt aaattttatc agagagctgc aaaaagagaa tgtatccaaa
     3721 agtggataca acagaggaaa cttgattatt taggaattcc tgtattttat ggatttggtc
     3781 tgactgattt caaaggaaga agttacagat tcatggtaat ggaaagactt ggaatagatt
     3841 tacagaaact cctggaccag aatggtggtt ttaaaaaatt aaccgtccta caacttggca
     3901 tcaggatgct ggatgtactg gaatatatac atgaaaatga gtatgttcat ggtgatataa
     3961 aagccgcaaa tctactgttg gattttacaa atccagaccg ggtttatctt gcagactatg
     4021 gactttccta cagatattgt cccaatggga accacaaaca gtatcaggaa gatcccagaa
     4081 agggccataa tgggacaata gagtttacaa gtttggatgc acacaaagga gtggccccgt
     4141 ccaggaggag tgatgttgaa atccttggtt actgcatgct gcactggctc ttcgggaagc
     4201 ttccttggga agcaaagctg gacgaccctg tggctgtcca gactgctaaa accaacctgc
     4261 tggatgaact cccagagtcg gtgcttaagt gggctccttc tggaagcagt tgcagtgaac
     4321 ttgtcaagta cttgatgtat gttcataatt tagcttatga tgacaagcca gactatcaga
     4381 agctcaagaa aattttgaat ccagatggag tacctttagg gccactggaa ttttccacta
     4441 aagtacagag tgtccatgtg cggactccag cccaacaaaa agttgattca ccaaaggcta
     4501 caagaaaacc agccaatgag ttcccagcta agttcccaaa gaaagtccac agggagacaa
     4561 gagcaaggca gagagaggag caggaagaca gccagcccac aatgctgcag agcaggccgg
     4621 cagcaccgga aaactcaagg acaaggaaaa tacatgaata ttctgatatt ttcagtgaga
     4681 tgcagagtct tcaacaaaca cccagctata tgagtttcca aggttcatat tgcaagccct
     4741 atctggactg cactagaaga gatcccatca ggaagccaag atccctgcct cggtacagac
     4801 acacgcccac gggtaatctg ggagtcacag acttggaaag ttctccaagg ttttggcctg
     4861 caatttttca gttgactctg agtgaagaga caaaggcaga tgtgtattac tatgggatca
     4921 ctatattctg tctgctgata tttgtatttc ttgctttgta ttttctctaa tgggccgcga
     4981 cgatgtttaa acttcgaaac gcgtgaattc ctcgagggcg gccgctctag agtcgacggg
     5041 ccgcggtaac aattgttaac taacttaagc tagcaacggt ttccctctag cgggatcaat
     5101 tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     5161 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     5221 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     5281 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     5341 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     5401 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     5461 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     5521 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     5581 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     5641 ggttttcctt tgaaaaacac gataatacca tgaccgagta caagcccacg gtgcgcctcg
     5701 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5761 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     5821 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5881 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5941 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6001 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6061 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6121 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6181 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6241 ggtgcatgac ccgcaagccc ggtgcctgac ccgggcgggg cgcgtctgga acaatcaacc
     6301 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6361 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6421 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6481 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6541 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6601 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6661 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6721 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6781 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6841 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     6901 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6961 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt tttccagtca cacctcaggt
     7021 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7081 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     7141 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     7201 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     7261 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     7321 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     7381 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     7441 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     7501 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     7561 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7621 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7681 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     7741 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     7801 attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7861 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7921 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7981 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8041 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8101 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8161 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8221 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8281 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8341 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8401 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8461 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8521 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8581 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8641 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8701 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8761 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     8821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10021 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10081 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10141 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10201 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
    10261 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10321 tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
