LOCUS       sequence-227945-       10460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged Itprip in lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Cheng LC, Baboo S, Lindsay C, Brusman L, Martinez-Bartolome S, Tapia
            O, Zhang X, Yates JR 3rd, Gerace L
  TITLE     Identification of new transmembrane proteins concentrated at the 
            nuclear envelope using organellar proteomics of mesenchymal cells.
  JOURNAL   Nucleus. 2019 Dec;10(1):126-143. doi: 10.1080/19491034.2019.1618175.
  PUBMED    31142202
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/19491034.2019"; journalName: "Nucleus"; date: "2019-12"; 
            volume: "10"; issue: "1"; pages: "126-143"
COMMENT     Sequence Label: pLV-EF1a-Itprip-V5
FEATURES             Location/Qualifiers
     source          1..10460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        194..405
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          406..1344
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1301..1321
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3049..3090
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3227..3776
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3371..3388)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3598..3617
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3779..4378
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3779..3798)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4275..4295
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4402..4990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4455..4475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4873..4884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5197..5830
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5958..5974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5958..5974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5971..5993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5982..5998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6006..6012,6013..6030,6031..6036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6051..6072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6189..6206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6360..6948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6440..6459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7119..7910,7911..7979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7742..7761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7980..8084)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8132..8266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8169..8188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8223..8242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8434..9067
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9114..9239
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9736..9969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10154..10198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
       61 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      121 gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt cttcactcga
      181 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      241 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      301 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      361 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
      421 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
      481 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
      541 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
      601 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
      661 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
      721 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
      781 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
      841 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
      901 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
      961 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     1021 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     1081 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     1141 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     1201 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     1261 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     1321 caaagttttt ttcttccatt tcaggtgtcg tgaggatccg ccatcggccc gcccgccgcc
     1381 atggccatgg ggctcttccg cgtgtgtctg gtggtggtga cggccatcat caaccacccg
     1441 ctgctgttcc cgcgggagaa cgccacagtc cccgagaacg aggaggagat catccgcaag
     1501 atgcaggcgc accaggagaa gctgcagctg gagcagttgc gcctggagga ggaggtggct
     1561 cggctggcgg ccgaaaagga ggcactggag caggtggcgg aggagggcag gcagcagaac
     1621 gagacacgcg tggcctggga cctctggagc accctctgca tgatcctctt cctgatgatc
     1681 gaggtgtggc ggcaggacca ccaggagggg ccctcacctg agtgcctggg cggtgaggag
     1741 gatgagctgc ctgggctggg gggcgccccc ttgcagggcc tcaccctgcc caacaaggcc
     1801 acgcttggcc acttttatga gcgctgcatc cggggggcca cggccgatgc agcccgtacc
     1861 cgggagttcc tggaaggctt cgtggatgac ttgctggaag ccctgaggag cctctgcaac
     1921 cgggacaccg acatggaggt ggaggacttc attggcgtgg acagcatgta cgagaactgg
     1981 caggtggaca ggccactgct gtgccacctt ttcgtgccct tcacaccccc cgagccctac
     2041 cgcttccacc cagagctctg gtgctccggc cgctcagtgc ccctggatcg ccagggctac
     2101 ggccagatca aggtggtccg cgccgatggg gacacattga gctgcatctg cggcaagacc
     2161 aagctcgggg aagacatgct gtgtctcctg cacggcagga acagcatggc gcctccctgc
     2221 ggcgacatgg agaacctgct gtgtgccaca gattccctgt acctggacac gatgcaggtc
     2281 atgaagtggt tccagacggc cctcaccaga gcctggaagg gcatcgccca caagtacgag
     2341 ttcgacctgg cctttggcca gctggacagc ccggggtccc tgaagatcaa gttccgttca
     2401 gggaagttca tgcccttcaa cctgattcct gtgatccagt gtgatgactc ggacctgtac
     2461 tttgtctccc accttcccag ggagccctct gagggcaccc cagcctccag cacagactgg
     2521 ctcctgtcct ttgctgtcta tgagcgacac ttcctcagga cgacactaaa ggcactgccc
     2581 gagggcgcct gccacctcag ctgcctgcag atagcatcct tcctgctctc caagcagagc
     2641 cgcctgaccg gtcccagcgg gctcagcagc taccacctga agacggccct actgcacctc
     2701 ctactcctcc ggcaggccgc cgactggaag gcggggcagc tggacgctcg tctgcacgag
     2761 ttgctgtgct tcctggagaa gagcttgctc cagaagaagc tccaccactt cttcatcggc
     2821 aaccgcaagg tgcctgaggc catgggactc cctgaggccg tgctcagggc cgagcccctc
     2881 aacctcttcc ggcccttcgt cctgcagcga agcctttacc gtaagacact ggactccttc
     2941 tatgagatgc tcaagaatgc cccagcgctc attagcgagt attccctaca tgtcccctca
     3001 gaccagccta ccccaaaaag cggtgggccg ggcgggggtg gcgggggagg taagcctatc
     3061 cctaaccctc tcctcggtct cgattctacg tgagtttaaa cttcgaaacg cgtgaattcc
     3121 tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct
     3181 agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga
     3241 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3301 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3361 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3421 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3481 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     3541 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     3601 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     3661 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     3721 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     3781 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3841 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3901 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3961 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4021 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4081 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4141 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4201 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4261 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4321 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     4381 cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     4441 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4501 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4561 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4621 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4681 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4741 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     4801 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4861 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4921 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4981 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     5041 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     5101 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     5161 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     5221 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     5281 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     5341 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     5401 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     5461 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     5521 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     5581 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     5641 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5701 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5761 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5821 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5881 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
     5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8101 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
     8161 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8221 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     8281 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     8341 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     8401 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     8461 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     8521 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     8581 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     8641 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     8701 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     8761 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     8821 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     8881 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8941 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9001 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9061 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9121 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9181 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     9241 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     9301 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9361 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9421 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9481 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9541 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     9601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     9661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     9961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10021 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10081 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10141 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10201 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10261 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10321 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    10381 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10441 cggatctcga cggtatcgcc
//
