LOCUS       sequence-22796-e         309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 309)
  TITLE     Klein lab unpublished plasmids
REFERENCE   2  (bases 1 to 309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2FLAG
FEATURES             Location/Qualifiers
     source          1..309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..63
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     polyA_signal    113..247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(137..156)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     191..210
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgatttaaag ctatggatta caaggatgat gatgataagg attacaagga tgatgatgat
       61 aagaattcaa ggcctctcga gcctctagaa ctatagtgag tcgtattacg tagatccaga
      121 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      181 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      241 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtgggg
      301 aggtttttt
//
