LOCUS       sequence-227968-        4611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF7 transcription factor, part of the Human
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF7
FEATURES             Location/Qualifiers
     source          1..4611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2175..2274)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2291..2310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2292..2310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2315..2331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2315..2331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2433..2452)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2444..3253
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2511..2530)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3400..3988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3889..3908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4142..4159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4318..4345)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4437..4523)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4572..4594
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4586..4603
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4587..4603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtgccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatggaggt
      121 ggtaacattt ggcgatgtgg ctgtgcactt ctctcgggag gagtggcagt gtctggaccc
      181 tggccagagg gccctctaca gggaagtgat gctggagaac cacagcagtg tggctggact
      241 agcaggattc ctggttttca agcctgagct gatctctcgg ctggagcagg gagaagagcc
      301 atgggtcctc gacctgcagg gagcagaggg gacagaggca ccaaggacct ccaagacaga
      361 ttctacgatt aggactgaaa atgagcaggc ctgtgaggac atggacatcc taaaatcaga
      421 atcctatggg acagtggtca gaatctcccc acaggacttt cctcagaatc ctggctttgg
      481 agacgtttct gattctgagg tctggttaga cagtcatctg ggcagtcccg ggctgaaagt
      541 gacaggcttt accttccaaa ataactgttt gaatgaggag actgtggttc ccaagacctt
      601 caccaaggac gcaccccagg gatgtaagga gctgggaagc agcggcctgg attgtcagcc
      661 tcttgaaagt cagggagaga gtgcggaagg gatgtcccag agatgcgagg agtgtggcaa
      721 aggcatcaga gccacttcag atatcgctct gcattgggaa attaatacac agaaaattag
      781 cagatgtcaa gaatgccaaa aaaagttatc tgactgcttg caggggaaac atacaaataa
      841 ctgccatgga gagaagccgt acgaatgtgc agagtgtggg aaagtcttca ggctctgctc
      901 gcagcttaat cagcatcaga gaatccacac gggagagaaa ccctttaaat gcactgagtg
      961 tggaaaagcc ttccgcctga gctcaaaact tattcagcat caaagaatcc acactgggga
     1021 gaagccctac agatgtgagg aatgtggaaa agcttttggt cagagctcaa gcctcatcca
     1081 ccatcagaga atccacacag gagagaggcc ctatggttgt cgtgagtgtg ggaaagcctt
     1141 cagccagcag tcgcagctgg ttagacacca gagaactcac actggggaga ggccctaccc
     1201 ttgcaaggag tgtgggaagg ccttcagcca gagctccacc ctagcccagc atcaaaggat
     1261 gcatactggg gagaaagctc aaattctaaa agcctcagac agtccaagcc ttgttgcaca
     1321 tcagagaatt cacgctgtag agaaaccatt taagtgtgat gagtgtggga aagcttttag
     1381 gtggatctct cgcctgagtc agcatcagct gattcacact ggagagaagc cttataaatg
     1441 caacaagtgt acaaaagcct ttggttgtag ttcacggctt attcgccatc agagaactca
     1501 cactggagaa aaaccattta aatgtgatga gtgtggcaaa ggctttgttc agggctcaca
     1561 ccttattcag catcagcgaa tccacactgg agagaaaccc tatgtgtgta atgactgtgg
     1621 aaaagccttc agtcagagtt ccagccttat ttaccatcag agaatccata aaggagagaa
     1681 gccctacgaa tgcctccaat gcggaaaagc cttcagtatg agcacacagc ttacaataca
     1741 tcaaagggtt cacactggag agaggcccta taaatgtaat gaatgtggga aagccttcag
     1801 tcaaaactca acccttttcc aacaccagat aattcatgca ggggtgaagc cctatgagtg
     1861 cagtgagtgt ggaaaagcct tcagccggag ctcatatctt attgaacacc agagaataca
     1921 cactagggcc cagtggtttt acgaatatgg gaatgccctg gaagggtcca cctttgtgag
     1981 ccgtaaaaag gttaatacta taaagaaact gcatcagtgt gaagactgtg agaagatatt
     2041 taggtggcgt tcacacctaa ttatacacca gagaattcac accggggaga agccttataa
     2101 atgcaatgac tgtggcaaag cttttaatcg tagctcaagg cttacccagc atcaaaaaat
     2161 tcacatggga ttggacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2221 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2281 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2341 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     2401 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2461 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2521 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2581 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     2641 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2701 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2761 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     2821 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     2881 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     2941 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3001 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3061 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3121 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3181 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3241 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3301 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3361 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3421 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3481 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3541 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3601 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3661 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3721 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3781 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3841 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3901 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3961 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4021 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4081 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4141 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4201 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     4261 tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa
     4321 aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt
     4381 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
     4441 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
     4501 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
     4561 atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc t
//
