LOCUS       sequence-228085-       10346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for 
            dicotyledonous plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR1CB0011
FEATURES             Location/Qualifiers
     source          1..10346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        438..783
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     726..746
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             874..4977
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4990..5010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5017..5019,5020..5022,5023..5736)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5062..5083)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5323..5342)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5670..5691
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5749..6001
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6049..6073
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(6229..7377)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(7713..8301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7793..7812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8472..9263,9264..9332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9095..9114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9333..9437)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9524..10155
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10247..10271
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg
       61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
      121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
      181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
      241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
      301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
      361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
      421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
      601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
      661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac
      841 aacattacaa ttacatttac aattatcgat acaatggaca agaagtactc cattgggctc
      901 gatatcggca caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc
      961 aaaaaattca aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc
     1021 gccctcctgt tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg
     1081 cgcagatata cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag
     1141 atggctaagg tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag
     1201 gataaaaagc acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat
     1261 gaaaagtacc caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct
     1321 gacttgcggt tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc
     1381 atcgaggggg acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt
     1441 cagacttaca atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa
     1501 gcaatcctga gcgctaggct gtccaaatcc cggcggctcg aaaacctcat cgcacagctc
     1561 cctggggaga agaagaacgg cctgtttggt aatcttatcg ccctgtcact cgggctgacc
     1621 cccaacttta aatctaactt cgacctggcc gaagatgcca agcttcaact gagcaaagac
     1681 acctacgatg atgatctcga caatctgctg gcccagatcg gcgaccagta cgcagacctt
     1741 tttttggcgg caaagaacct gtcagacgcc attctgctga gtgatattct gcgagtgaac
     1801 acggagatca ccaaagctcc gctgagcgct agtatgatca agcgctatga tgagcaccac
     1861 caagacttga ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa
     1921 attttcttcg atcagtctaa aaatggctac gccggataca ttgacggcgg agcaagccag
     1981 gaggaatttt acaaatttat taagcccatc ttggaaaaaa tggacggcac cgaggagctg
     2041 ctggtaaagc ttaacagaga agatctgttg cgcaaacagc gcactttcga caatggaagc
     2101 atcccccacc agattcacct gggcgaactg cacgctatcc tcaggcggca agaggatttc
     2161 tacccctttt tgaaagataa cagggaaaag attgagaaaa tcctcacatt tcggataccc
     2221 tactatgtag gccccctcgc ccggggaaat tccagattcg cgtggatgac tcgcaaatca
     2281 gaagagacta tcactccctg gaacttcgag gaagtcgtgg ataagggggc ctctgcccag
     2341 tccttcatcg aaaggatgac taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct
     2401 aaacactctc tgctgtacga gtacttcaca gtttataacg agctcaccaa ggtcaaatac
     2461 gtcacagaag ggatgagaaa gccagcattc ctgtctggag agcagaagaa agctatcgtg
     2521 gacctcctct tcaagacgaa ccggaaagtt accgtgaaac agctcaaaga agattatttc
     2581 aaaaagattg aatgtttcga ctctgttgaa atcagcggag tggaggatcg cttcaacgca
     2641 tccctgggaa cgtatcacga tctcctgaaa atcattaaag acaaggactt cctggacaat
     2701 gaggagaacg aggacattct tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg
     2761 gagatgattg aagaacgctt gaaaacttac gctcatctct tcgacgacaa agtcatgaaa
     2821 cagctcaaga ggcgccgata tacaggatgg gggcggctgt caagaaaact gatcaatggg
     2881 atccgagaca agcagagtgg aaagacaatc ctggattttc ttaagtccga tggatttgcc
     2941 aaccggaact tcatgcagtt gatccatgat gactctctca cctttaagga ggacatccag
     3001 aaagcacaag tttctggcca gggggacagt ctccacgagc acatcgctaa tcttgcaggt
     3061 agcccagcta tcaaaaaggg aatactgcag accgttaagg tcgtggatga actcgtcaaa
     3121 gtaatgggaa ggcataagcc cgagaatatc gttatcgaga tggcccgaga gaaccaaact
     3181 acccagaagg gacagaagaa cagtagggaa aggatgaaga ggattgaaga gggtataaaa
     3241 gaactggggt cccaaatcct taaggaacac ccagttgaaa acacccagct tcagaatgag
     3301 aagctctacc tgtactacct gcagaacggc agggacatgt acgtggatca ggaactggac
     3361 atcaatcggc tctccgacta cgacgtggat catatcgtgc cccagtcttt tctcgcagat
     3421 gattctattg ataataaagt gttgacaaga tccgataaaa atagagggaa gagtgataac
     3481 gtcccctcag aagaagttgt caagaaaatg aaaaattatt ggcggcagct gctgaacgcc
     3541 aaactgatca cacaacggaa gttcgataat ctgactaagg ctgaacgagg tggcctgtct
     3601 gagttggata aagccggctt catcaaaagg cagcttgttg agacacgcca gatcaccaag
     3661 cacgtggccc aaattctcga ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg
     3721 attcgagagg tgaaagttat tactctgaag tctaagctgg tttcagattt cagaaaggac
     3781 tttcagtttt ataaggtgag agagatcaac aattaccacc atgcgcatga tgcctacctg
     3841 aatgcagtgg taggcactgc acttatcaaa aaatatcccg cgcttgaatc tgaatttgtt
     3901 tacggagact ataaagtgta cgatgttagg aaaatgatcg caaagtctga gcaggaaata
     3961 ggcaaggcca ccgctaagta cttcttttac agcaatatta tgaatttttt caagaccgag
     4021 attacactgg ccaatggaga gattcggaag gcaccactta tcgaaacaaa cggagaaaca
     4081 ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag tccggaaggt cctgtccatg
     4141 ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg gaggcttctc caaggaaagt
     4201 atcctcccga aaaggaacag cgacaagctg atcgcacgca aaaaagattg ggaccccaag
     4261 aaatacggcg gattcgattc tcctacagtc gcttacagtg tactggttgt ggccaaagtg
     4321 gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac tgctgggcat cacaatcatg
     4381 gagcgatcaa gcttcgaaaa aaaccccatc gactttctcg aggcgaaagg atataaagag
     4441 gtcaaaaaag acctcatcat taagcttccc aagtactctc tctttgagct tgaaaacggc
     4501 cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc
     4561 tctaaatacg ttaatttctt gtatctggcc agccactatg aaaagctcaa aggatctccc
     4621 gaagataatg agcagaagca gctgttcgtg gaacaacaca aacactacct tgatgagatc
     4681 atcgagcaaa taagcgaatt ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag
     4741 gtgctttctg cttacaataa gcacagggat aagcccatca gggagcaggc agaaaacatt
     4801 atccacttgt ttactctgac caacttgggc gcgcctgcag ccttcaagta cttcgacacc
     4861 accatagaca gaaagcggta cacctctaca aaggaggtcc tggacgccac actgattcat
     4921 cagtcaatta cggggctcta tgaaacaaga atcgacctct ctcagctcgg tggagacagc
     4981 agggctgacc ccaagaagaa gaggaaggtg ggttcgatgg tgagcaaggg cgaggagctg
     5041 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5101 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5161 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     5221 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5281 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5341 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5401 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5461 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5521 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5581 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     5641 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5701 gggatcactc tcggcatgga cgagctgtac aagtgagctt gtcaagcaga tcgttcaaac
     5761 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     5821 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     5881 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     5941 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     6001 cgacgctact attgtcttct gcacgaagtg gtttaaacta tcagtgtttg acaggatata
     6061 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     6121 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga
     6181 tcaggcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
     6241 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
     6301 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
     6361 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     6421 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     6481 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     6541 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     6601 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     6661 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     6721 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     6781 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     6841 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     6901 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     6961 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     7021 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     7081 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     7141 aagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     7201 cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa
     7261 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     7321 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     7381 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     7441 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     7501 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     7561 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     7621 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggatctgcc
     7681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8581 ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta
     8641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9361 atttatcaag gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9421 caaatagggg ttccgcgcac gaattggcca gcgctgccat ttttggggtg aggccgttcg
     9481 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
     9541 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
     9601 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
     9661 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
     9721 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
     9781 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
     9841 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
     9901 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
     9961 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    10021 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    10081 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    10141 agcccggtga gcgtcgcaaa ggagatcctg atctgactga tgggctgcct gtatcgagtg
    10201 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    10261 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    10321 actggggtgg atgcagtggg ccccac
//
