LOCUS       sequence-228094-       11555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral dual fluorescent protein reporter, HypoxCR, detects 
            hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Le A, Stine ZE, Nguyen C, Afzal J, Sun P, Hamaker M, Siegel NM, Gouw
            AM, Kang BH, Yu SH, Cochran RL, Sailor KA, Song H, Dang CV
  TITLE     Tumorigenicity of hypoxic respiring cancer cells revealed by a 
            hypoxia-cell cycle dual reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Aug 11. pii: 201402012.
  PUBMED    25114222
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Aug 11. pii: 201402012."
COMMENT     Sequence Label: HypoxCR
FEATURES             Location/Qualifiers
     source          1..11555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    2240..2464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2453..2470
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2486..2611,2612..2617,2618..3328,3329..3331,
                     3332..3334))
                     /codon_start=1
                     /product="EGFP destabilized by fusion to a mutated version 
                     of residues 422-461 of mouse ornithine decarboxylase, 
                     giving an in vivo half-life of ~1 hour"
                     /label=d1EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV
                     I"
     primer_bind     complement(2660..2681)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3009..3028
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3268..3289
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3331..3340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3373..3411)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        4636..4939
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4940..5143
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5272..5281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5278..5985
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5423..5441)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5691..5710
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6016..6345
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             6346..6369
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6389..6888
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6424..6447)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6821..6840
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6909..7508
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6909..6928)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7405..7425
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7522..8110
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7575..7595)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7993..8004)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8317..8950
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9079..9095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9079..9095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9092..9114)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9103..9119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9127..9133,9134..9151,9152..9157))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9172..9193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9310..9327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9481..10069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9561..9580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10240..11031,11032..11100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10863..10882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11101..11205)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11253..11387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11290..11309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11344..11363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatcagggc gcgtggggat accccctaga gccccagctg
     2221 gttctttccg cctcagaagc catagagccc gggccatccc cagcatgcct gctattgtct
     2281 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
     2341 ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct
     2401 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
     2461 acagtcgagg ctgatcagcg agcttctaga tcacattgat cctagcagaa gcacaggctg
     2521 cagggtgacg gtccatcccg ctctcctggg cacaagacat gggcagcgtg ccatcatcct
     2581 gcgccgccac cgccggcggg aagccatggc taagcttctt gtacagctcg tccatgccga
     2641 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     2701 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     2761 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     2821 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
     2881 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     2941 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     3001 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     3061 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
     3121 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     3181 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     3241 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     3301 ccccggtgaa cagctcctcg cccttgctca ccatggtggc aagcttctag tgatctgacg
     3361 gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac cagatctcga
     3421 caagaggacc tgttggagcc cacgtatgca ctgtggctcg acaagaggac ctgttggagc
     3481 ccacgtatgc actgtggctc gagaaaagct cggtaccgat ctcttaatta agagctcacg
     3541 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     3601 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga
     3661 gatttagaat gacagaatca tagaacggcc tgggttgcaa aggagcacag tgctcatcca
     3721 gatccaaccc cctgctatgt gcagggtcat caaccagcag cccaggctgc ccagagccac
     3781 atccagcctg gccttgaatg cctgcaggga tggggcatcc acagcctcct tgggcaacct
     3841 gttcagtgcg tcaccaccct ctgggggaaa aactgcctcc tcatatccaa cccaaacctc
     3901 ccctgtctca gtgtaaagcc attccccctt gtcctatcaa gggggagttt gctgtgacat
     3961 tgttggtctg gggtgacaca tgtttgccaa ttcagtgcat cacggagagg cagatcttgg
     4021 ggataaggaa gtgcaggaca gcatggacgt gggacatgca ggtgttgagg gctctgggac
     4081 actctccaag tcacagcgtt cagaacagcc ttaaggataa gaagatagga tagaaggaca
     4141 aagagcaagt taaaacccag catggagagg agcacaaaaa ggccacagac actgctggtc
     4201 cctgtgtctg agcctgcatg tttgatggtg tctggatgca agcagaaggg gtggaagagc
     4261 ttgcctggag agatacagct gggtcagtag gactgggaca ggcagctgga gaattgccat
     4321 gtagatgttc atacaatcgt caaatcatga aggctggaaa agccctccaa gatccccaag
     4381 accaacccca acccacccac cgtgcccact ggccatgtcc ctcagtgcca catccccaca
     4441 gttcttcatc acctccaggg acggtgaccc ccccacctcc gtgggcagct gtgccactgc
     4501 agcaccgctc tttggagaag gtaaatcttg ctaaatccag cccgaccctc ccctggcaca
     4561 acgtaaggcc attatctctc atccaactcc aggacggagt cagtgagaat attatcgata
     4621 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4681 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4741 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4801 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4861 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4921 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4981 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     5041 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     5101 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     5161 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctacta
     5221 gaggatcgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgccaccatg
     5281 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     5341 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     5401 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     5461 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     5521 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     5581 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     5641 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     5701 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     5761 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     5821 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     5881 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     5941 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggata tccatcacac
     6001 tggcggccgc tcgagatgaa cccatccatg aagcaaaagc aggaggagat taaggaaaac
     6061 atcaaaaact ccagtgtgcc tcgccggacc ctcaaaatga tccaaccatc cgccagcggc
     6121 agcctcgtgg gtcgcgagaa cgaattgagc gccggactga gcaagcgcaa gcacagaaac
     6181 gatcatctca ccagcactac aagctctcca ggcgtgatcg tgccggagag ctccgagaac
     6241 aagaacttgg gcggtgtgac acaagaaagc ttcgacctga tgatcaagga gaaccctagt
     6301 agccaatact ggaaagaggt cgccgagaag agacggaaag ccctcgacta caaagacgat
     6361 gacgacaagt agtctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6421 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6481 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     6541 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6601 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     6661 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6721 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6781 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     6841 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     6901 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6961 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7021 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7081 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7141 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7201 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7261 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7321 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7381 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7441 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7501 gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     7561 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7621 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7681 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7741 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7801 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7861 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     7921 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7981 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8041 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8101 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     8161 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     8221 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     8281 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     8341 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     8401 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     8461 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     8521 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     8581 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     8641 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     8701 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     8761 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8821 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8881 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8941 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     9001 tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta
     9061 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9181 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9241 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11221 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
    11281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    11341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    11401 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    11461 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    11521 gtatttgtta aatatgtact acaaacttag tagtt
//
