LOCUS       sequence-228117-        5714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL18 - pNST3
FEATURES             Location/Qualifiers
     source          1..5714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5300..5319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5301..5319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5329..5351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5545..5564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5664..5686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tattctacac attcacaaag tttactacac
     2281 tatatataat ttacccaaca aacacttatt ttactgcatt attcagtata ttatcttacc
     2341 tataaatgtg tatcatcatc atcaataacg cgattatttg tgctgaagga ttatatattc
     2401 aaaatgatct agttatatat gtcacatgat tgccgttaac aagacacatt tgaagaagct
     2461 aagcaagaaa aacggacact tttgcgactt gttacataat ttaacttata ggtcaaaaga
     2521 atttgattag tcattgcaac tacgtgtgga tgtcactttc tattcaacca aaactcacaa
     2581 tattatatga tctagttttg tcgtattact gatttgtatt ataaaatgtt atttaatttg
     2641 aattctacgt agatattgct catgcatgat agtatgtatc taaactattc aaataactaa
     2701 ctacgtggat attttataat ccaagtaaaa agcagaaagt gggtaactac gtcagtatga
     2761 ctatactttt atcggaattg cttgacatcc aaacttttgc tatgcttcac caaccaatgc
     2821 agtttcactt aattattaac tattgactat gtcttattaa gttagcacta attcgttaat
     2881 cattcaaaac gttatttgat tgaattacat attacactct ctttctgcat caccactcac
     2941 accatatgca actataacca actcatcaca ttcaaatgta ttaattggat tttggtgcga
     3001 gattaaaaat tgaaaggaaa caaaatatga taatgggata aaatcttgaa cggaaactca
     3061 aactaatcct cataaggtat aacaaaataa caatttaagc taagcacaac aacatacaag
     3121 ttcgaccttt tcctttgatg atccagccca acagttctct tatatctcaa accattcgac
     3181 catttgagcc aaactagcta aacctgcagg aatcaaaacc aacaaagatt cagattagct
     3241 aaaccggttt catccctttg tcacatgact cacatccgtc ttctacataa cgatttctaa
     3301 tgatgtgagc tcttaacttg ctccagcaag atcatcaact ttggagcacc ttcaatgatt
     3361 tagttaacat gttagataaa ttaaatattc ttgtttcaat atatatcaac tttagtgtaa
     3421 aagccttaac attctcttaa atatttaatt tatttctcct tatttcgatt taatgacaaa
     3481 tgtgaattaa tttttgtgat atttttgttc gaaattagtt ttcagttaat aacatacatg
     3541 tgagcatggg acacacatga tttaacaaaa gggaatgacg aaatgatata tcaaaatatt
     3601 agtatgggaa caaattacga ggtgaaactt cacactcaac tcaattaaaa ctagaataaa
     3661 gaaatggaaa aagtgaaaga atgagaggtc aaatgtggtt aatcattatg tggtattagt
     3721 taatccatca attgtgtacc caaaagcatg attaagcata gaatttagag aaacaaaaca
     3781 tcattattaa tgttgaaaca caaagatccc atcaacagac aaatgataag tacagtgcat
     3841 gtagggtaac aacttttatg tacatgttat atacttatat tatataataa gaaaacgatt
     3901 aaagtgtcat tgctccagcc tctatttgta aatcatatta tatcagtatg cttaattcca
     3961 ataattaagt ccataactaa aatatataca catatatgta tgttaaatgg ttgaatatat
     4021 acatatattt tcataaacaa atattgctaa ttaattcagt tatttgtgta cataatccaa
     4081 ctatcacctt tttagctgga agtggatatt ccaacatgtc agtctgtcac tcccacattc
     4141 atactctcta ttctttttag ctatttcaat atctacggtt aaatattaat ggctatatag
     4201 ccttaccctt cattttagtt tttttttggt attcgcataa ccatcgaata ctcaaactta
     4261 ctatgtaaga tggtctgaat aactatttcc gatttaagat gaatagctag attgaaatat
     4321 acatgcacta attggacatg cactaaaggc agaggtgaat taaatgatga aatgaagatg
     4381 aagtgtcaca cttgtgcaaa aagcatgtcc cctgctcttc tccgcttgtt tcaatttctt
     4441 tgactttcat cacgtttttg tcacttaaat acaccaaaaa atatagtaca attaaacatc
     4501 gaaaatcgtc caaaaagaag aaaaaaaatc atggaaagtt ctttcgttaa tgttacacac
     4561 attatcttga ttaggtgaca ccagatatta gaataaaaat gatagattat gaaaagaaaa
     4621 aaaaaattga tgtattttta ggatacatcg aaaggaatga acataccaaa aacatgggaa
     4681 aaaatagata actaattaac atggtagaat gtagatgacg tagatcatga aacgagtgtg
     4741 tgatatatta atgaaaatta ttttaatata cgtagctata ttagaaaata atttacattt
     4801 attttcttct aaacaaatct atactttata tttacataca ttagtaaaga ccaaaacaca
     4861 tggaattcaa attctgcaat aagtaattgc aagaaaacac aaagattaat cccccactaa
     4921 acccgtttat ttacgttagt atttttccgt tttatacatt acacatgaca tgacattaca
     4981 cgtcaaaaga aatatgtctt acgtcagaac ttacgtatga tcaaactcga tttaaacata
     5041 gaaacatctg tttactaaat tatactaatt tcataaagac actttaatgc atgaacttct
     5101 ttgtttaaat aacaatttcc cccttttggg ggctatgtct cgtcgagtcc taccaccatt
     5161 ataaattatc tcatcgtttg ctttcttttt tttaagttgt aaccatttcc actcgtaatc
     5221 atacaacttc tctactcttc tagagcaaaa acccaaaaat atattgctat cttcgttaaa
     5281 acatgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg
     5341 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5401 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5461 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5521 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5581 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5641 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5701 accgaaacgc gcga
//
