LOCUS       sequence-228120-        5784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL27 - pPXY
FEATURES             Location/Qualifiers
     source          1..5784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5370..5389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5371..5389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5399..5421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5615..5634)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5734..5756
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tctgcatgtt gaatttgtgg tggtcatgta
     2281 ttgtacgaat tgaactgttt atttgaataa ttagctcatt ggactgtaga ttatcatcac
     2341 ttagatgaca aatcatttca ttcgccaaaa atataaaatc caaaagtgtc acaacaaatg
     2401 tcaaatacaa taaacggtgg tatttaagaa gaaaaataaa tctaaaaatg agtcacattt
     2461 gaaataacat tttgtttata taaatggcct cactcccatc aatcggtttc tttctctctt
     2521 tatttcatcc tctttgttta cttttgaaga atgttcaatt ctttcatcat tctcttgtag
     2581 aaacttttat aattatttta cttttctttt ctactttatt ttgtttacgt ctcgtgtact
     2641 tccagattta acaatttcct ctaaattcat gtattttaca tatacaagca aaattgagta
     2701 ttattgctct gttacaaatt ttgaataccg aggaattcta cattttattt tgcttttttt
     2761 tttttttaac aacaggaatt ctactttaag tataactcag ttcatacaaa acccacagaa
     2821 aaatgaaaaa ttgaaggatg ttcattcaat aatagatatt ctaaagtcag aaacctacaa
     2881 tttgaagtat aatattttta aatctcttaa gtcagaattt ttgtttatga catcaacaaa
     2941 aaaatattct aaatctctaa tacactcata catatattgc aaaatataat ttcttgatca
     3001 catttcatgt aatagaaatt cagttggggg taaaaccgtc atttgagaaa aggctaaaca
     3061 taccatactg ttgttgcgaa aaagacacgc gtggccaaaa aacactaaca aaagagaaat
     3121 gtaacgacgc atgcctctat acattttctt ttcattattg cactctactt gttgtttttt
     3181 aaaacatccc aaaattttta ggatcgttga tcgtttatag gacgagtttg taaaacgtat
     3241 aatcgttcta attgagatat tatcggttct tccactacat cacgtagaaa aagaagttga
     3301 ttttggactt ctaattaacc acacttacaa agtgattata ttcgaaccaa tgcagttgca
     3361 aactatgatg caacgggtaa agaattaacg atatataata gaaatgaatg tgtgtgattt
     3421 caaaatatag tatagcatta agctcactca cgtacgtacg gagtctttaa gatatgtatg
     3481 cataagcgat aatttaggtg attagttggg aaataatgaa ttcctttttg gaactttatg
     3541 gatcatcgaa taaatgtgag atatttaatt cacccaacct taatgatgaa aagtgaatct
     3601 ataatggcaa gcaaccatca tgatacaaac cttatatgcc cttaagtcaa tttctacagc
     3661 tataacttta tatatatagc attgacaaaa gataatttat ttttttcatt gtgttactgt
     3721 gtttttagta aagaaaagaa aaaatatcat aagaaataga catcgtgacc ccaaaaaaaa
     3781 tacgtataag caaaaggcat agcttggatt tcctagtagt atcaaataaa taaatgagac
     3841 aaaatatttc tagtagaacg ggattacggg accatgtgtt tgagatattt tatgaaaatc
     3901 aatagaaata gtaaatagaa aaatatgaga atataacttg tgttcataat atgatgatgt
     3961 atatacaaga ttaacatgta aattaaagaa tgaagagtcc tagctcgaca cattattttt
     4021 agtagagcag aaccaggtga ttgagataat tcatgttcat aattatgaat tcacgagtaa
     4081 ataaactcgt aaatatcaag aatgtaagaa aaacgatttt ttttttcttc ttcttatagt
     4141 agtctaatag ttgaaagatt tatcatgtaa gcgtagaatt taatgttgaa attatctcac
     4201 tacgatttga atcatgctga gaattaagct aatgcttagg atggaatgtt gtagtctatc
     4261 gtataaaata actttatttt agactacgat aaaattaaaa tcataaaact ctcaactcca
     4321 atcctaatct aatcaagttt ttctaaggtt ggtagacaat agtagatttt cagtgatcgt
     4381 aagtattact tggcatatat ataaacgaac tgaaaaataa gtcccaatgt aatatcattt
     4441 cactaaaagt aataaagaga tgcacgcgta tatatctata cgtatgaatg gtctgatgaa
     4501 aggataaaaa aagaatggtg taataaattt gttataaaat cttgaatctt ttaggcgcga
     4561 gattgttgtt cgaaaaaaaa aaaccgtaat tcatatatat gtaaaaactt atatacgcac
     4621 acatatatgt agtgggcatg agagttagtg gagagacaga ggattttagc tttattggat
     4681 cctgtgagtg actgggataa atacagtcca ctttcgctta ttgtgtatct atgtacttgt
     4741 acatctgcat gcgtatacat tccattgaac attcaatttg tatgcaaaag gttttttaaa
     4801 aagaatacca tagtggaaaa aaaagaattt taatgaggag aagaaattaa aggacaaaga
     4861 aaagatacta gcaaatctaa ttaatcggtt tttaatatga gtggcattgt gtgatttggt
     4921 ttctgatttt acccaatact tgtatgatgg caaaacaaaa acaaaatcta caattatagt
     4981 atatacgttt tattttggtt catacttaat aaaggtaaca aacagaaaaa agtactagta
     5041 tacgaagtac agtaatttga aaaaagtaag aaaagtaaaa gaagagtgga aaatggattg
     5101 agtttttttt ttctctttgc ttttgttttc ttccccacac aaaaaccata atgccttaac
     5161 caacaaaagc cagaccatta gacgcactcc acaatcatca ccttcatcat gacttcttct
     5221 tctcttctct tcttccatct tcttcttctt caaaccctta aatccaccat tgtcaacact
     5281 aacacctttc tcaacaatat caccacactt cctcaaacaa cttcacttta atttctttct
     5341 aaaagctaca acatgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg
     5401 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5461 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5521 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     5581 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5641 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5701 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5761 caccgtcatc accgaaacgc gcga
//
