LOCUS       sequence-228178-        5203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL26 - pSCR
FEATURES             Location/Qualifiers
     source          1..5203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4789..4808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4790..4808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4818..4840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5034..5053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5153..5175
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcgaccacca ccgtcaacaa ttttgaatcc
     2281 attctcaaag ctttgccaat ctgcgttcga aattcgaaaa ccctaacgac tttggagatt
     2341 caaattgagc caaatctcta tgaaaagtgg aaatttacct ggaagtccga ttgagaggag
     2401 aggattgacc ggagagagtc cggagaaaga tgggagtgaa ggagaagaga ttgggagacg
     2461 aagagtaacc ggacggcgag aagacgagga aggtaatcgg agtgatgcgg tggagggaag
     2521 agctgatttg agagaagaca tactccagac tctgctcacg gaatctgtag gagtcgccat
     2581 tgccatggac attggaatcg ccattagatt gtgatcctct gcaacaaagc ggattttgct
     2641 ggtgttgaat ggataaggga tagaggaaga ggactttgtt tatcagaaac cttttgatgg
     2701 gccttaatgg gcctataaac tgtaactctg tagcgctttg ccaacaagag actttttaag
     2761 gtttttgttg ccaaacagat atttgcattt gggctatgta atgttagaat tattttataa
     2821 tgtatgctat tgctagatat tgtttaagtg catttgtgat ttacaaacat ttcattttta
     2881 ttttggtttt aatgagcatt tctattatag agactttgat gttaataaat ggtgttctaa
     2941 gatatattaa aatattttat atactttctt aaaattggat aaattttggg aaaatcctta
     3001 atatcagtta aattgaagat aaagagtatt aaaaaaaact atgtagtaaa atacatttca
     3061 cattttttgt gtataatagt acatggtatt cgttaagatc actcaaaaat taacaaatta
     3121 agtctaaaag ggcagaaaag actattcaaa tatggacttg gagaaagaca ttcagctttt
     3181 tacgctgaga aactttcata ttgagccgtg tgtttgtgtt gtgaagagaa gtaataaaaa
     3241 ataatttgaa gtgaaaaagg agaagaaaaa ataagatcgt agaaagcgtg gatggtttct
     3301 tcttgggttc actgccatgc gattattaaa ttggccatgg ggctagtgtt tgacgtacaa
     3361 aagtctaaaa attgtcagtc aaacaggtcc aaaactttgt aagaaaaata atataataat
     3421 agcaaatttt ctaaaaattg ttaaaaaaag aacaaaaggg aaaagatgag gatgcagatg
     3481 aaagcaaaat gtcaaacact agtttcagat tttatcggga actggggttt gacagttggt
     3541 gtatgtatgt aatggcctct catcaaaaca tgtgcatctt tttccttttt tgttatttac
     3601 tgttttagct ctacgtcttg tccaattcct ctcaagtaaa atgcctttaa tatgatacta
     3661 atatacaagg ggactaatgc tttttccctt ttcttatcct tgttttgtct aaatctttac
     3721 ttggattcct ttatttttct cctctcttta gattagtacg gtttaaggaa taccatcttt
     3781 ctaattttag cacaaaattg caagttggtg ccccatctta gtaagcacat cgtaccacac
     3841 tttgattgtg tgagagactt cttcatccca tctctcatac caaacctaaa tcaaatgact
     3901 agtggtgcaa cctgctgact ccatatgacc ataactaata aatcggttta tgaatccaac
     3961 tcatgtagct ctatagaata gaaacccatt catttcacat aatgaactga atctgacatt
     4021 ttatttacat catttactac tcaattttgt aattagcaag atcatctttt tcattattca
     4081 acaattttga tattccataa tttattaact ttgtcataca tcataatatt ctgaaatttt
     4141 gttatatatt gtaccggttc cacgaaatag agctctatta ttatagacca aacaaacaaa
     4201 atattatctt cttgtggtta gttcgagaga gaggtcaaga agaaacgaaa tggatcggca
     4261 aacggaagac gtcaaacaca caacgacgaa cattttccga tcacccacct aatctcttcc
     4321 catttttatt atttttcaaa actcaaatta attaagaaga aaaaaacaga aacagagaga
     4381 gaaagagtta agatgaatag agatagaaag agtcattaaa tgtacgaagc gacattcaca
     4441 ataattcgaa aggtggaaga cgacttagat acggccaggc ttcactgtcc tcctcgtcct
     4501 cctcaattac ccctaacccc tttttccggg attcatctcc aacccacatc cttccaaatt
     4561 ctcaccccct cactgagttt ttgctttttc tcctcatcgg agatcgtgaa gacgatcaag
     4621 taatttaaga atcccaccat tgataaaaga gtctagcttt tctactacca aacctttttc
     4681 tgtttggaaa ttttcgattt tggatttaac ccttttctta ccttatttat aaccatgcaa
     4741 tctcacgacc aacaaccctt caatctccaa catgagaccg aattctcgcc ctatagtgag
     4801 tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4861 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4921 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     4981 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     5041 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5101 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5161 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cga
//
