LOCUS       sequence-228180-        5630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL35 - pAPL
FEATURES             Location/Qualifiers
     source          1..5630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5216..5235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5217..5235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5245..5267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5461..5480)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5580..5602
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tgctcttagt ttgcttcaac aacaattagc
     2281 taaaagttaa acattagctt tacttggttt aactaaaaac aaaaactttg gtttcttttt
     2341 ttcaaagcaa aatacaattt agcttaaaat cattattgaa aacattaata aagaaagatg
     2401 agaacttaaa tgattttagg gcggatgatt tgctaatgct aatatgcatc atatcactat
     2461 gaatcaattt tgtagataat agtgatgatg attgtgaaaa cacaaatatc gtgataatat
     2521 aagtaggaat aatatatgtg tgtatctata ctcaaaaagt ggtaagaaaa aataaaaagt
     2581 ctagtaaact atgtaaatgt ggctggccaa gtttatacgg ttggcatcta tcgcgggagt
     2641 tgtattttca ttttttaata ttgttggcgc aataagaatc tatctttatt catcaaagag
     2701 gagaagaatc gattccaatt ctcatgcaat ttgaactttg atttaatatt taaattcaaa
     2761 agttataacc attttcttat tttatcttgg tcagaattgt gtgcggtaat tgattaattt
     2821 aatttattgt acaaataatt cgtttcacag ccaagatttt taacatttct ggcaaagaaa
     2881 aacactgttt ttttatttat tccaagaaat taatatctga aaaatgtgtt accatgtata
     2941 gctgcacaat cgtcagtcct acaaaagcca aaaaaggtta tagccccagc aagctttaac
     3001 caataaatta aaatggagaa aagaagaaaa acatatggta aaaataaaaa gaataaaaga
     3061 gctttaataa gtagaagaag aggtccccaa cgttaactct atctttacac attgatgcat
     3121 aattctccac atattaatag tccgtgttta taatatgtat aactttaaaa aattgttcca
     3181 tattcttatg ttaatttttt tgttttaatt caatattcat tgactggtat tggtctacat
     3241 tttaacatgt acatgctttc ccttaattaa tacttaatat ataacctcta ataaatttct
     3301 gattcaattc aaattttatt cttagataat attctctctt ttttgtgtgt gcatatcagc
     3361 ctcagccata ttcattgact tattcttgtt ctatgtaaat atgctatggg acctcccact
     3421 catatatatt taggttgacc atttatttta catgcatgca attgaattta ggggttaatc
     3481 taaatcgttg cgtatatgaa ttatatatga ttttagtttg tatattgaaa aatgcatcgt
     3541 cgttctatac ataagctagt ttagtagcta atagggcaat attaacaaag ttgattttct
     3601 ggtttgtagt gtccatacaa gtaatttagt taattatctc gtttgttcac ctttttgttt
     3661 cttttttctt ttgctagcta gttaattgcg gatgtaaact acatctttta ctaatcgatg
     3721 aatggaaaac gtttttgtcg tagacaaata ttccaccgta cctaacggtt ttggattgca
     3781 tgtatataat aaatttgttc caaaaatata cggtctatta atttataacg aagctcatca
     3841 ttgcttagaa ttgtatattc acctcataat tagccaaaaa ggtttaccct accataaatg
     3901 ataaaatccc tagccttgtg gaatttgttt cgttaagata tttgtatgta tgcctccaat
     3961 tacgatatat gacgatgtag acaaactata agtctagtat acgactctcc tcgtgcggtc
     4021 ttaattgaat agaatacata gaactttaat atattcactt taaaagttta tattttccta
     4081 taaagaaaat atttacaaat ctttgattac tatttactcc aaatcaagca tgattaagaa
     4141 aatagtatat agaacaattg tatgataaat ttggatttaa aaattacttc taaattataa
     4201 ggtagtaaag aaaaagaaac caaaaatata agaataatag atgtgcaata tctttgattc
     4261 aggtgaatta tattttaact tagatatctc aagtcatttt aatgtttata tcaggaaata
     4321 aagaaataaa aaccatcatg caacaaatca tgcatatata ttagatacat ccgtaaccgc
     4381 tttttttcgt cttttttttt tgtttttttc gtcgtcacat ccataaccgt tttgatatga
     4441 gatctccgat gttcaaaatt caatttcccc caaagatatc aatttaatat ctaatatata
     4501 ctattatttt aagtttgcgc agtaatttaa taatctgaga cagacatgat ttacttaatg
     4561 tatacgtata gtggctttta cacacatttt atattgaaag aaattcaaag gtattcaagc
     4621 taagaggata aaaaacacaa atgaaaaaga tagcgacagt atcttttcac aaaggaacgt
     4681 ctgtgtacta gtctgaaact ttagttctct tccttactaa cgataaaaca tatttaacta
     4741 aaccataatt tatttacaaa caaaacaaaa aagacgagag agagaaaaaa aagccgtcat
     4801 ctgatctctt caaaaacgca tcgtattctc tcacattttt gcatcacatt cgtttcttga
     4861 atcccctctt tcagagagag aaagagagtc agagagagag agagagagaa tgttccatgc
     4921 taagaaacct tcaagtatga atggttcata tgagaacaga gctatgtgcg ttcaaggcga
     4981 ttcaggcctt gtcctcacca ccgaccctaa accgcgtttg cgttggaccg tcgaactcca
     5041 cgagcgtttt gtggacgccg tcgctcagct cggcggcccc gacagtatga acacacatac
     5101 acaaatatat attcaatcca tttaaccaat tataaatgtg ttttttcaaa gtttttgatc
     5161 atttctattt gcagaagcga ccccaaagac gattaaacat gagaccgaat tctcgcccta
     5221 tagtgagtcg tattacctgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5281 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5341 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     5401 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     5461 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5521 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5581 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
