LOCUS       sequence-228185-        4964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL36 - pAt2g38380
FEATURES             Location/Qualifiers
     source          1..4964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4550..4569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4551..4569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4579..4601)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4795..4814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4914..4936
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tttgaagcag aagaagacga gtcttcgatg
     2281 gggaagaaga gagtgaagtg aaaaagagag agagagagag attagggagg aaccaaagtc
     2341 ttgtgtttgt gaattgaaac tggtttgata aagacgtgtc ggaacacgcg ccacggtcgt
     2401 ctcgtgaaca gtgttagaaa gtcatgtttt tttttttgtc tcaatttaaa tttgaaatta
     2461 gcaaaaattt cgcgtctttt cccctcttgg atcatataat tgctaatctc aattcttaaa
     2521 ttgggattaa ggaataacct ttaaacggct aaaactatct gaatgttttg atattttatc
     2581 agattgttaa aaagtgatca aaaaaaaaca aagcaatata tttatcttca catgcctatt
     2641 caatttaagt gacgtgttgt tatttatcac aattaactga cagaattttg taaaacgtta
     2701 ccatgtgact gttttttttt tttttcttat cacaaacaga aaattatttc cgttccaatc
     2761 tttcttgcca tggaagaagc tatgttagaa aatgagctct agcttaccgg taatagggaa
     2821 accagtcttg acaaccatcg agaagagggt gacaacgata catgagcgat ggatagcaca
     2881 taagaataaa atgatgaagg gttcaagcaa aaaaaaactt ggtaagagtt tgaacccaaa
     2941 acatcagatt tgtcatcttt taggtgagac aaaaaagtct tgataacaat gtgtgccgga
     3001 cacagttttt ttatctagaa gattaaactt tgatttttct cacctaaaag gtgacaataa
     3061 agtgagctat gtagagtgtc gtcaattatt tgtccatctt cttgtttgtt tttttaattt
     3121 cttctttttg tccaaccatt agactaatat tttcattttg tttctttctc ttttgtcttc
     3181 gctttcatct tgttttcagg aatgactaat gcattcgaat gttttttctt ttccttttat
     3241 gtgcattcct actttatgaa acaattagtt ttcaattcaa tttcggtgat agttttagcc
     3301 atttctgtcc actttggttg tcttagttag tttattgatg agaagaaaag acaaggagca
     3361 aaagtattag attaggttaa ccaaaaagtc tagcatatgg actcgtatga aattgatatt
     3421 tgtatgtatt aggtaacttt gttttctcag agattattga gcaaaatgtt tataatttat
     3481 cgattagtga aggtggcttc ggaggctaca tttgcgttat ttacgggaaa tggaaggaca
     3541 aaacacaagt gctttctagt ttgaaaataa aatcaaagaa atattggtta aaatagaaca
     3601 cactttataa tcactgatca cataatatta cattattatg acttatacga tttacaaaaa
     3661 caattcaggt caaaggcact cgtcgatata acataaagtt ggacattgaa actgagaata
     3721 cgttggcgtt cgcggattta acatatattt ttttgtccat atttttatac cgtatcgtct
     3781 caacaattct atcatcaaca agaacatgtc actgataaaa acatgtttat cgcagcatga
     3841 tgatattata catagacata tcttacgttt aacatgaatc aacaaccata tctttagcga
     3901 ccctacacga attcaaattt gatatcaatt tcgtacaatt tctatcttac tactttgaaa
     3961 tttgtgtcgt ttataattat ttatcaaact caatgatgcg tattctattc tcagaaggag
     4021 tcatgtatgt cttgaaataa atctagaaag aagtataggc gaaattgaga accatctctt
     4081 catagaacta tacgtcgatt catagtaagt aaaaagtcta catctgcaat aaaaatgtat
     4141 ggagttagct agttttgaaa aatactatat acaattgaag tgaacatgaa aatcttaatt
     4201 gtttaattaa agcgcgcgaa aaaacacaat aataagttaa attatgtgca cataatagat
     4261 agcattgtca aaaggatcgg ttaatttatg atttcactaa tttttattat ccttcaacca
     4321 ctctagtaaa atacatattg ccttaaaaca aaacctacta gtcttaaaac gaaaaaagna
     4381 aaaaaaaaaa aaaaaaaact caatacctaa taatcaaaaa tagtactcaa gcttccctat
     4441 ataatgttcc ttcttggctt tagctaattc accatatacc cacactcaca agtcacaact
     4501 caaataatct ttgtcttcct cctacaaata acatgagacc gaattctcgc cctatagtga
     4561 gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4621 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4681 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     4741 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4801 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4921 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga
//
