LOCUS       sequence-228187-        5637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL37 - pWOX4
FEATURES             Location/Qualifiers
     source          1..5637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5223..5242)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5224..5242)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5252..5274)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5468..5487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5587..5609
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tggtaccttt ggttcatttg gttttgggag
     2281 cggattaaaa cgtagaaact acggtttcac aaacaactta tttaatttta agtaaagaga
     2341 aaataactcc ttttcttgaa atcttgataa aaaaaacaat caattgcaat ggtttatgtt
     2401 ccttgaataa gaagttagag tttgttcaaa aacaaacaaa acctaaggca aacctttgtt
     2461 cggttctcga tcagtcgatt tatatgaatg gatttctatt caacatatgt tctcttttct
     2521 tatttttcgg ccttgaatct cttatttttc tattttttcg ggtcctcaag attatccacc
     2581 tagatgtgtc tcactctttt catttcccta ttgcaggtca acgacgtttt atttaagctt
     2641 tttcccatag accgattacc catttatcag aattacttgg attaatggaa ttattctcga
     2701 tttaagtcga atttttcgaa gggtgctaca tatctcatca acaaacaata caaatcaaag
     2761 gaaggaagaa gagctaaaga gctaaaaaaa agatgaggag gagatcgaag taaacaaatt
     2821 ggaagaaaaa gagatccaac aatgattggt tgtgaacatg ttatgctagt ttatttcaaa
     2881 ttttgaaacc ctaaacctca aaccatcagt aatctgtcat taccgacata gtattacata
     2941 tggatcgaaa aataaacatg ttaaatatgt gtcctaaaaa aatacatatg aacagtggta
     3001 gaggcaagtg tagtggagga gggtcactgc atatgaatat cgacgatata gtatgggcca
     3061 tagataattg aagaaataac catctgatca cagcttgaca cacgaatcag gttatccatc
     3121 gtgtgatgac atttttcaaa aaatatgaat ggtattacca gtttcttgtt atgctttggg
     3181 ttcagtaaag ttccactcat tttccagtat ctgatgaatg atagagcagt ctttttagaa
     3241 ctttgagctt tcatattcct tccgtccaac aaagatgaat tttctatgga aaaaaattgt
     3301 cccacaaaga tacaaatttt gtatttttaa tataaatttt aaaattgttt acctgtttta
     3361 tcctaagtaa ataagtagat aaatattttt tattattttt aaaatatatt aaaaggttaa
     3421 aattgtcagt atacattatt ttttaaaaat gtgaaatttc tataaaaaaa tccatcttca
     3481 taggataaag atgttattaa ttttagctat tagtcatgat caacctacac tggtacttct
     3541 atatttcctc catgatatca atttttatgt tttaaccagt gatatgaact gtgactttcc
     3601 accttcataa ctttgatatc catatatatt agtgtaagtt tcgtactgta catatacttg
     3661 catcataact ggattagccg agactaaata attttgacta ttcttcacag ttcatactca
     3721 aggactctgt tctttgtaat tctagactac atatcaaata aaaagatgat gatatattag
     3781 tcaaaactca aaagtaatta gaagaaaaaa ctttgtaatt ccggaatatc atacgttact
     3841 acttttcatt agtaaaatac ccaaattact tgggaaattg aggttctgca gttcccgatg
     3901 tacaagagaa taacaataca atacactgat ctaaagtgaa tgggacgctt gacattgcca
     3961 aaccaaacca tgcttttgta tcaatatcta ataaacatat aagcttaaat ttataatgat
     4021 gtattattat caaaataaac aatgataata taacattaag atatttctag ccttttcttt
     4081 tccgcataaa agtctgtcct ggctcattat aaactaccac taatttaggc aaaatccgta
     4141 tcttaacaaa agtaataaaa gcaatttcga gtattgggat tggaagatac cttttcccct
     4201 tattcattct tcttctcatt cttcttttct ctaaataaag tagttgagac ttgagagcca
     4261 ccgaaaagca ttcgacatcc tatcattaaa gctaactttt ttcagtgttt tatttctaaa
     4321 ataatttctt aaaataaaga aaggtgagag agaaattatg tccctataca actagtagaa
     4381 tgaaagattg tcaagctttg gtttgggatt tttctaataa atcacatgca tttgttagaa
     4441 tttgatccca aaaaaaaaaa attcaaacct ttttcctgta aaagcaaacc tattggctcg
     4501 taggtcaatg tcaatctcaa tgaatagtaa agtcttttga tatcacgtat ctataacata
     4561 ttatttttag cgtggttcat gtccctcaac attctgccta aattatagga tctcaagaca
     4621 tagtactata gactacaact acaagtagag gaaaatttta taagagagat aaattatgcg
     4681 gtattcacgc atgacatgcg taagcatgaa actaaaattt ggttgataca ttactatgcc
     4741 gaatttaaag tagtgctcca tgcagacatg aacatatcag atatcacacg tacgcatgta
     4801 ttacgtgtat gtagagacac acgtacgcat atctatattc acacaatgag atttcacttt
     4861 ctcaaacaaa gatcactgtc ttcaattagt ttcaaatttg ttctttacct attgctttta
     4921 catttctgag tgtatatatt tacaataagg acaaggagat tttaaatgat aaaagtcaac
     4981 catatcccat catttggtct ttggtttccc gacgtcccac ttttgtaaaa atcatctgtt
     5041 cattttccat tctttttctt tttcctaccc aactggatat caccaatgat atcattatac
     5101 actaacacta tcatttcaca cttatcttcc tctatatata catagcttaa gtcttgtaac
     5161 gagaaaggca tgcatagcat ttgctagttt taacatatag ccaacatgag accgaattct
     5221 cgccctatag tgagtcgtat tacctggccg tcgttttaca acgtcgtgac tgggaaaacc
     5281 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5341 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     5401 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     5461 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5521 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5581 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
