LOCUS       sequence-228191-        8567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL28 - pSMXL5
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8153..8172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8154..8172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8182..8204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8398..8417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8517..8539
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tcttagattc gcactcgctc aacaaccgtg
     2281 agctctgata ccaatttgta aagatctaac ttcacttcaa tctcacaaac acacgaatca
     2341 acttcagcac taagaatcga attgatcaga actgaaagaa agtaaaagaa aagctgaaca
     2401 cagagaattt aacgagttca cgttccaaac cggaacgcta cgtctcgccg gagaaacctc
     2461 tccggaaatc cactagaact tggaaactta gtctctgtta caatgctttc aagctttctc
     2521 tcactacact cctctctctc tttgatctca acaaactctt atcccgatct aacggaatta
     2581 cagagaagaa gaagaagata tagttacata gctcatgcga aaagaccaaa acgccctcaa
     2641 gcttgttatg actttagtca gctcgcctca cgtgttatga ctttagtcag ctcgcctcac
     2701 gtgttatgac tttagtcagc tcgcctcacg ggttacgctt gaatcacaac agacttcgaa
     2761 ccctctcttt caccgagttt ctcccacact taaacccgaa cactaaaatc tgagtcattt
     2821 cttacaagta tctcagctat tctcaaaaga tcaaaccctc tttatgatcc ggtgggttcc
     2881 tttgtagact caattttcat aaaagcttga tcgatcgaga ttcatgcttc attgggatta
     2941 gagaagaaaa gagagagtaa tgatgattag tttctttatc ttttccatgc aaaagaagaa
     3001 aagcattaac atttgaaaaa aaggtaaaga aaataattaa cactcttttt ttttttttgc
     3061 ctttttttat cccttggatt taaccttctc tctttacctt tttttcccct tctcctttaa
     3121 tactttaatg atcactcctg tcccctttag atccgatgaa caccaaaaag cccttttcca
     3181 tataaaatta acatgcgttt tttcttcttt ctaatttcat atctattcta cagtctcata
     3241 cctagctaat atctttttga ataatttatt attataaaca ctaaacctca aaattaatgc
     3301 gtacatatat ttttcgcagc gatacgtaac acaaagtata aatacttttt aagtttttca
     3361 cgatgacata catatattag taatattaag cacgtaagtt ctatcacaaa tgacaataca
     3421 taatcggccc gtctatacta gtttgcgaga cactgattta gatccatatt cgtaaatatc
     3481 atagaacaat atataggtct accctttgcg ttgatatacc tttaacaaga gggatgtgtt
     3541 acatccacat ttaaagttaa tataggtatt gatgtgtcaa cactataatt aaagttgaat
     3601 ttttactgca ccagataata ataatttaaa taaataagta caaaaattaa ctagcacaaa
     3661 catttatagg caaaaagtat cttgcataat gtatatatat atatttttta aatgtatata
     3721 tgttgctctg ataagaagta agtagtagta ttgcagaaaa ttaaaggttc gccttttatt
     3781 cgttaatgcg ataaacaaat tagtggaaac cgtataatat ggaacgtaaa gttatgtgct
     3841 ttcattttca cactcccatg cattttcacc tttttcaatt ttttatttta gaattttgta
     3901 aaccttttcc tttgttaatt ttctcgccaa tatatccaaa caaaaaaaat caaacccgtg
     3961 tttttatttg gcattattgg tctgatcata tacttatgct agctaaacct aaacgtgttt
     4021 ttaactttca aactcgttat taaaataaat aaaaaatttc aaactctgat atttttcttt
     4081 cttattgatt ataatatatc gacaatcatc gttgcgatgt ttgatgtagt ttcagtttca
     4141 gagtttgtaa atacttcaca aggtagaaaa agaaataggt gtagtttatt attttcttct
     4201 ttaaaatttc caagttcaat attaattata actgtccaaa tatcaaatat taacaataat
     4261 ggtcaaaagc actatcaaga caccgaatcg attatgacac ctcatgggct ttaagtctta
     4321 acgattctat tgattgatct caaacttaga atagatttcc gttttcgatg aagttgctta
     4381 tatatctttt aatatataga ctcaagatgc atttgcaatg atggggtcat ctttgtattc
     4441 caaactattg ctggatatat gtaaggcaaa atgcaaacca aatcattaga actccaaact
     4501 ttgccacttt agctccttat cagtattaaa gaaaacttca tttgcatttt tcttcatcaa
     4561 tctttctcta aaagtatata ctttccttgc aaatagagta gtctcaatta aatatattta
     4621 tatttggtct ttctctcatt tcatgggtcc tatgggaata ttattcccca atgatttgga
     4681 ctctctccac cataataaga cccatcttgg cttctttgat tacacaaaag ttagaagtta
     4741 tgatcattac actaaactaa gagcaaagta gtgctccaat attcattagc taatatcatg
     4801 tagaaccaaa atctttagct ttctctttgc cttttttctt ttcttaatgt taaacatttt
     4861 tctgtgtttg ctattaatca tgagggtata taatctacaa attaattaaa tagtttagcc
     4921 aaaagcaacc catgatgtgt attagttttg gcatgaatta atttcaactc tagacgacat
     4981 ttttgacttc gaaggttaga aacaagatcg agtatgatat cacaaagatg tcttgattat
     5041 ctgtattagc aaagaattat gatagattag atttcttcat gcagtggtaa agcgtatatt
     5101 tttgtttgct atatttgttt tcgatgatca gaaaattcca aaaaataaaa cacagactta
     5161 tccactaaac cttttaaaag tgcagtactg ttaccagtta aaatgttatt caagtcgcag
     5221 tgataaaaat ctcaaaagta tatgacaatt ttcattcgta gcacctatat atttgcattt
     5281 ttacatcttc cacttcgaat ttattgattg aggcttcgac ctctactaca aatgttttca
     5341 cgaatatatc cattgcaaaa atttagccac agacgaacaa tgccgatacc gcattgatga
     5401 ctatactatt actaatctta tggtacgtag tatagcacat ttgttttagg attatagttt
     5461 gtaatgaaga actacagctg ttggaaaaaa aaaagagtta tccgacgata gaaaatgcag
     5521 catatagaat ttgtaatgaa aaactacgtc tttgagacga cgtaatctaa agcaaacact
     5581 aatcttctat cttgttaatc cgtttcattt tttttgctgt gagttctttt atcggtcaaa
     5641 caacaaatgc atggtggggt taacaccaaa gtccttttac cacgtggttt taaactaaat
     5701 tacttaatta tacacatgga taaagacata tatgttgatg aagagaatat aataaagccc
     5761 ttttaccatg aagtttcttt ttcgaatttt gaccgaaaag agattcaata agatccgtta
     5821 tatagatgag aagtgttatt gggaaataaa gcaagagtgg caagtggacg atagtgtgct
     5881 tgcacaatag tcgagagaaa aacacattat tttgagttta aggagtccat taacaccatg
     5941 atcaaaccaa ttactatcta ttcaaattca agattaatca ctaattttct agctttttga
     6001 gtaattacat tcgcatttga ttgttcttca cttaaatcaa cttaatttta tgatgaacca
     6061 caagactata tctatttaag tatttatagt aaaacttcaa tgtaagttct tgtaatgaaa
     6121 acaaagagcg tttttgtcca ctatctatat gttacaaact tacaatacaa ttcgtttaag
     6181 aatgtttggt gggagatttt cacatgcaac atatctaatg tgggcaagca agagattgct
     6241 tgaaatagac caatcctcga gcgatatccg cgggcaacca ttttaatctt ggtcccctta
     6301 gccgcttttt gaatcaaaat cttttaaagt ttctttattt catgaaatca atcaatatta
     6361 agaatttttt ggaaacatac aaaatattta tagttacagt agttagttcg tcattaccaa
     6421 tatgcaaagt tcgaaacatg tattaacaaa gatgattcta aagctaattt tgggcgacat
     6481 tctaagtttt tgcttctttt tatagttctt ttagttagtg gggaaaaaga agtattaaag
     6541 tgggttgttg ggatttttat ggtaaataac attgttggga atttttgtat cagacaactc
     6601 aaaaatatat tccctctgtt tcttaagtac tatatgtttt tgtgattttt gcacaattta
     6661 agtaattatt tgcaatacca aattaagttt caatttatta attgattatt ataagtttat
     6721 aactaagatt tacattaaat tgaatttaat aggtatgagt aagaaaatga aatattactg
     6781 tcacaaaaat aaatcttaac ccttttttgt ttgtaccgaa cacatttcat tttgttttcc
     6841 ttcttccttt acttcttctc tttatgcaat tcttgaggat cgttctcgtc acacgttagc
     6901 acttagcctt agcagaccag aggagctaca agagtttttt tttgacaaat acctttcact
     6961 taagaccaaa attgattgaa gtttatataa aataataaat gattatgtgg tataaacaaa
     7021 aggagggaga gatgagacag aaggttgctt ttgcagtatt ccgggaatgc aatgctgtct
     7081 ctctccgagg attggcgtga gtgagtgaga gagagagaga gagagtctca ttcatctctt
     7141 ttgtccttct tgtaattaat tggttttcac tattacaata aaaatgatac tataattttc
     7201 gataaaaata tgcctttatt ggagattaaa caaatgcgag tctaaataac ctttttagga
     7261 ccaacctaag atatacataa aaagatcaag aagatctttg tttggtcact cctacgcaca
     7321 tcaatgcaaa aagagacaaa acttctctta accaagaaat gaataaattt ttacttttta
     7381 caaatgtcca attcaattag catgcatttt aaaaacgatt ttttctccac aagttattaa
     7441 tgtgtaaatt agtatatatt tgaaaaaaag acagcttttt tttatgctct tattcgctaa
     7501 taatgaccaa aaattaaaag agatatgatg agtacactga agctgcactg ttactgtaaa
     7561 accaaatgag aggagaggaa caaagaaaag agagaagaca aaaaagttga gtgaaatgca
     7621 aaaaatcaaa agcatacaaa agctgctcat ttgtccaaaa agaaacccat cataaagtaa
     7681 aagcattata atgagatcaa aacccttcga aaaatttaaa aataaaaacc aactttttct
     7741 taaaaaataa ttcaacaata ggaacagaaa acacacagca tgaaataagt ataaaggaac
     7801 cagcttggtc taatttcctt atctgtctaa aactctctta aacctctgtc tcttctctct
     7861 ctttcttctc ttacttatct tcttcttctc ttctctcatt tcttgagttc tctctcaaga
     7921 ctcttctttt cttcagtctt caaacataag aaaggaagtt ccttttgttt cttctgtttt
     7981 agttctcttg tgggtttatc tatttggggt ggtggttaaa ggagggagct ttgggattag
     8041 aagtttttca gtgaaatcag aaagctttcg agagaggttt ttttcaattt caaaacgtgg
     8101 gggtgggggg ttagggttag ggtttacaag ttaacatgag accgaattct cgccctatag
     8161 tgagtcgtat tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8221 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8281 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     8341 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8401 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8461 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8521 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
