LOCUS       sequence-228206-       11233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0003
FEATURES             Location/Qualifiers
     source          1..11233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5006..5026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5033..5035,5036..5038,5039..5752)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5078..5099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5339..5358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5686..5707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5765..6017
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6425..6449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6758..7387
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7816..8889
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8955..9149
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9357..9461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9462..10256
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10427..11015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10916..10935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11151..11175
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggacaagaa
      901 gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga
      961 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     1021 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     1081 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     1141 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     1201 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     1261 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     1321 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     1381 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     1441 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     1501 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     1561 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     1621 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     1681 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     1741 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     1801 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     1861 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     1921 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     1981 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     2041 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     2101 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     2161 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     2221 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     2281 gatgactcgc aaatcagaag agactatcac tccctggaac ttcgaggaag tcgtggataa
     2341 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     2401 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     2461 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     2521 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     2581 caaagaagat tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     2641 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     2701 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     2761 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     2821 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     2881 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     2941 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     3001 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtctcc acgagcacat
     3061 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     3121 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     3181 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     3241 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     3301 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     3361 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
     3421 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
     3481 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     3541 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     3601 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     3661 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     3721 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtttc
     3781 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     3841 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct
     3901 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     3961 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     4021 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga
     4081 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     4141 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     4201 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     4261 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     4321 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     4381 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     4441 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     4501 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     4561 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     4621 gctcaaagga tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     4681 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     4741 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     4801 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     4861 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     4921 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     4981 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgggtt cgatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gagcttgtca
     5761 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5821 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     5881 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     5941 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6001 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagcatcttc attcttaaga
     6061 tatgaagata atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta
     6121 tatgggaaag aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa
     6181 aagtcccaca tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag
     6241 tagtgattgc cgttaatttg agagtccagt ttaagagcta tgctggaaac agcatagcaa
     6301 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     6361 tcgctttacg aggatgcaca tgtgaccgag ggacacgaag tgatccgttt aaactatcag
     6421 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     6481 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     6541 cacagggttc ccctcgggag tcagccgtgc ggctgcatga aatcctggcc ggtttgtctg
     6601 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     6661 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     6721 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     6781 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     6841 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     6901 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     6961 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     7021 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     7081 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     7141 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     7201 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     7261 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     7321 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     7381 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     7441 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     7501 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     7561 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     7621 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     7681 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     7741 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
     7801 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     7861 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     7921 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     7981 tcccggcaac cgccggcagc cggtgcgtcg tcgattagga agccgcccaa gggcgacgag
     8041 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     8101 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     8161 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     8221 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     8281 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     8341 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     8401 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     8461 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     8521 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gattacagaa
     8581 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     8641 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     8701 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     8761 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     8821 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     8881 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     8941 aaaagcttct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     9001 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     9061 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     9121 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaacagctg
     9181 caaaaagcgc ccacatcaag gctccgagtg cgcggaaccc ctatttgttt atttttctaa
     9241 atacattcaa atatgtatcc gctcatgaga caataaccct gatggcctgt gcataactgt
     9301 ctggccagcg cacagccgaa cagctgcaaa aagcgcccac atcaaggctc cgagtgcgcg
     9361 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9421 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatggctaaa atgagaatat
     9481 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
     9541 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
     9601 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
     9661 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
     9721 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
     9781 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
     9841 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
     9901 tgaataacga tctggccgat gtggattgcg aaaactggga agaggacact ccatttaaag
     9961 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
    10021 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
    10081 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
    10141 cgctcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
    10201 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagctgt
    10261 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11041 ggttcctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg
    11101 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11161 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
    11221 atgtactggg gtt
//
