LOCUS       sequence-228207-       11421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0004
FEATURES             Location/Qualifiers
     source          1..11421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5006..5026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5033..5035,5036..5038,5039..5752)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5078..5099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5339..5358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5686..5707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5765..6017
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6613..6637
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6946..7575
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8004..9077
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9143..9337
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9545..9649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9650..10444
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10615..11203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11104..11123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11339..11363
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggacaagaa
      901 gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga
      961 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     1021 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     1081 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     1141 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     1201 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     1261 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     1321 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     1381 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     1441 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     1501 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     1561 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     1621 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     1681 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     1741 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     1801 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     1861 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     1921 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     1981 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     2041 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     2101 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     2161 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     2221 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     2281 gatgactcgc aaatcagaag agactatcac tccctggaac ttcgaggaag tcgtggataa
     2341 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     2401 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     2461 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     2521 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     2581 caaagaagat tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     2641 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     2701 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     2761 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     2821 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     2881 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     2941 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     3001 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtctcc acgagcacat
     3061 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     3121 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     3181 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     3241 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     3301 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     3361 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
     3421 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
     3481 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     3541 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     3601 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     3661 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     3721 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtttc
     3781 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     3841 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct
     3901 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     3961 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     4021 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga
     4081 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     4141 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     4201 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     4261 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     4321 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     4381 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     4441 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     4501 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     4561 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     4621 gctcaaagga tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     4681 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     4741 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     4801 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     4861 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     4921 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     4981 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgggtt cgatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gagcttgtca
     5761 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5821 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     5881 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     5941 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6001 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagggagcat cttcattctt
     6061 aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga agcaggccca
     6121 tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg aaaacaatct
     6181 tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac agctagagtc
     6241 gaagtagtga ttgccgttaa tttgagagtc cagtttaaga gctatgctgg aaacagcata
     6301 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     6361 tttttgcaaa attttccaga tcgatttctt cttcctctgt tcttcggcgt tcaatttctg
     6421 gggttttctc ttcgttttct gtaactgaaa cctaaaattt gacctaaaaa aaatctcaaa
     6481 taatatgatt cagtggtttt gtacttttca gttagttgag ttttgcagtt ccgatgagat
     6541 aaaccaatac gctttacgag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa
     6601 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     6661 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     6721 atgccaacca cagggttccc ctcgggagtc agccgtgcgg ctgcatgaaa tcctggccgg
     6781 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     6841 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     6901 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     6961 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     7021 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     7081 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     7141 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     7201 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     7261 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     7321 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     7381 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     7441 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     7501 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     7561 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     7621 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     7681 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     7741 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     7801 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     7861 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     7921 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     7981 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     8041 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     8101 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     8161 gcaaagaatc ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag ccgcccaagg
     8221 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     8281 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     8341 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     8401 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     8461 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     8521 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     8581 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     8641 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     8701 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     8761 ttacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     8821 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     8881 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     8941 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     9001 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     9061 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     9121 aaggtcgaaa aagcttcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     9181 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     9241 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     9301 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     9361 aacagctgca aaaagcgccc acatcaaggc tccgagtgcg cggaacccct atttgtttat
     9421 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataacccgcc tggcctgtgc
     9481 ataactgtct ggccagcgca cagccgaaca gctgcaaaaa gcgcccacat caaggctccg
     9541 agtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat
     9661 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     9721 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     9781 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     9841 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     9901 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     9961 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
    10021 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
    10081 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc
    10141 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
    10201 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
    10261 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
    10321 cgtccggtcg ctcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
    10381 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
    10441 ttagctgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10741 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    10801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    11041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11221 ctttttacgg ttcctgctcg gatctgttgg accggacagt agtcatggtt gatgggctgc
    11281 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    11341 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    11401 cgtttttaat gtactggggt t
//
