LOCUS       sequence-228238-       10205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-hygro-mCherry-RAMP4
FEATURES             Location/Qualifiers
     source          1..10205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1952..1976
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2107..3285
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2338..3276
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3233..3253
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3302..3320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3365..4072
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3510..3528)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3778..3797
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(4303..4327)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4358..4946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4411..4431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4829..4840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5046..5069)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5396..5415
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5481..6506
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5481..6506
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6724..6957
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7029..7150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7066..7085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7120..7139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7190..7325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7346..7364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7374..7391)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7374..7390)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7383..7405)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7532..7987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7619..7638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7829..7850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8013..8117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8118..8186,8187..8978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8336..8355)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9149..9737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9638..9657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9891..9908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10025..10046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10061..10066,10067..10084,10085..10091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10099..10115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10104..10126
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10123..10139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10123..10139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10158..10178
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10160..10178
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccac
     1981 catgggaacc gacattgatt attgactagg cttttgcaaa aagctttgca aagatggata
     2041 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     2101 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     2161 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     2221 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     2281 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     2341 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     2401 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2461 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2521 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2581 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2641 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2701 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2761 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2821 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     2881 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     2941 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3001 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3061 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3121 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3181 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3241 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat tagcttggta
     3301 ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttggta ccgagctcgg
     3361 atccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3421 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3481 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3541 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3601 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3661 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3721 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3781 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3841 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3901 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     3961 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4021 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggtggatc
     4081 tggaggttct ggtggaatgg tcgccaagca aaggatccgt atggccaacg agaagcacag
     4141 caagaacatc acccagcgcg gcaacgtcgc caagacctcg agaaatgccc ccgaagagaa
     4201 ggcgtctgta ggaccctggt tattggctct cttcattttt gttgtctgtg gttctgcaat
     4261 tttccagatt attcaaagta tcaggatggg catgtgatct agacccagct ttcttgtaca
     4321 aagtggttga tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat
     4381 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4441 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4501 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4561 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4621 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4681 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4741 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4801 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4861 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4921 gatctccctt tgggccgcct ccccgcctgg acctgcagga attctgcaga tatccggact
     4981 agtgatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     5041 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     5101 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     5161 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     5221 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     5281 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     5341 tcgctttctg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5401 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5461 tcgacctgca tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5521 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5581 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5641 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5701 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5761 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5821 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5881 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5941 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     6001 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     6061 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     6121 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     6181 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6241 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6301 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6361 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6421 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6481 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag
     6541 aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa acgcgtcgcc cgccccacga
     6601 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     6661 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6721 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6781 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6841 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6901 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6961 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7021 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7141 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     7201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7261 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7321 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7381 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7441 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7501 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7741 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7861 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7981 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8041 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8101 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8161 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8221 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8281 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8341 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8401 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8461 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8521 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8581 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8641 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8701 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8761 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8821 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8881 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8941 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9001 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9061 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9541 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9781 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9841 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9901 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9961 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10021 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10081 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10141 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    10201 cttaa
//
