LOCUS       sequence-228244-       11351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo single-strand annealing substrate composed of two truncated 
            GFP genes, separated by a drug selection marker, and flanked by 
            mouse hprt targeting sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M
  TITLE     Genetic steps of mammalian homologous repair with distinct mutagenic
            consequences.
  JOURNAL   Mol Cell Biol. 2004 Nov;24(21):9305-16.
  PUBMED    15485900
REFERENCE   2  (bases 1 to 11351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-11"; volume: "24(21)"; pages: "9305-16"
COMMENT     Sequence Label: hprtSAGFP
FEATURES             Location/Qualifiers
     source          1..11351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2223..2239
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2223..2239
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     enhancer        3566..3945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4223..5239
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5163..5185
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5247..5266
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5294..5303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5300..5302,5303..5305,5306..5818)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(5345..5366)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        6056..6553
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6091..6114)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6486..6505
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6572..7171
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6572..6591)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7068..7088
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7629..7648)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7694..7749
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7748..7767)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     8682..8703
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8907..8926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(10707..10726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10708..10726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10733..10750)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10733..10749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10742..10764)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10891..11346
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10978..10997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11188..11209
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtggatcc gcggccgcta attcgattct tctgtaaaat tatcttatta
     2281 gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg
     2341 tgacctacac acacacacac acacacacac acacacacac acacaggggg gtggattctg
     2401 agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta
     2461 tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc
     2521 agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt
     2581 tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc
     2641 cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt
     2701 ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa
     2761 ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag
     2821 caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt
     2881 ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat
     2941 atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta
     3001 taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg
     3061 agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg
     3121 acagccagag ctatacagag aaatcctgcc tcaaaaaacc aaaaataaag tgtatatact
     3181 aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt taatattttt
     3241 ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact
     3301 ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg
     3361 taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt
     3421 attatctaaa cgtaattaaa tgttgatatt attgaagcat ttatcagggt tattgtctca
     3481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3541 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca
     3601 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     3661 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     3721 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     3781 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     3841 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3901 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     3961 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     4021 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggsgcg cgccrggsgg
     4081 ggsggggsgg ggsgrggggs ggggsggggs grggsggrga ggkgcggcgg cagccaatca
     4141 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4201 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     4261 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     4321 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     4381 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     4441 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     4501 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     4561 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     4621 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     4681 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     4741 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     4801 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     4861 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     4921 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     4981 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     5041 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     5101 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     5161 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     5221 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     5281 tggcaaagaa ttcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5341 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5401 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5461 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     5521 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5581 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5641 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5701 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5761 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5821 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5881 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5941 gaagtgagaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     6001 caatgccctg gctcacaaat accactgaga tctgatatca tcgatgaatt ctaccgggta
     6061 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     6121 tggcgctaca caagtggcct ctggcctcac acattccaca tccaccggta ggcgccaacc
     6181 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     6241 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     6301 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     6361 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     6421 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     6481 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     6541 tccgggcctt tcgaccgatc cagccgccac catgaccgag tacaagccca cggtgcgcct
     6601 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     6661 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     6721 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6781 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6841 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6901 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6961 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     7021 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     7081 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     7141 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     7201 cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc cccgccgacc
     7261 ccgcacccgc ccccgaggcc caccgcgggg gacacaccga acacgccgac cctgctgaac
     7321 acgcggcgca gttcggtgcc caggagcgga tcgaaattga tgatctatta aacaataaag
     7381 atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt
     7441 acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata
     7501 aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg
     7561 atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga
     7621 tctatagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     7681 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     7741 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     7801 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     7861 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     7921 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     7981 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     8041 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagctttaa
     8101 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     8161 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     8221 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     8281 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     8341 ggcaactaca agacccgcgc cgaggtgaag ttcgataggg ataacagggt aatccgcatc
     8401 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     8461 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     8521 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     8581 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     8641 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     8701 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     8761 tctagatcat aatcagccat accacatttg tggaggtttt acttgcttaa aaaacctccc
     8821 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     8881 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8941 tttttcactg aagcttgttt ttatttgtat ctttaaattt tatgatagat gttatagtgt
     9001 actctcctct cccctcccct cccctctcct cctcagcagg atcttgttat gtagccctaa
     9061 gggatctaat acttactctg tagtccaggc tgtccttcag ctcatgagac acaccatcat
     9121 gcttggcatg tttcttgttc tgaaaggtat ctggttttaa ttttggttga gctggtgtgt
     9181 tggtgtatat ctgtaatctc agcacaggca agtagagaca aggattatga ttacagtcca
     9241 gcctgggcta tttagaggga ttctctttaa tctttcctca tgccccaaaa tcttaccttt
     9301 ggtatatgaa aaatagtctc cacttctgca aaatattgct ttatgaagta agaattccct
     9361 tcatagagac aaggaatgtg tcctgtaaaa gtttaatgtg taagaagtat ttgttataaa
     9421 agataaatat tcagaatctt ctttttaatt cctgatttta tttctatagg actgaaagac
     9481 ttgctcgaga tgtcatgaag gagatgggag gccatcacat tgtggccctc tgtgtgctca
     9541 aggggggcta taagttcttt gctgacctgc tggattacat taaagcactg aatagaaata
     9601 gtgatagatc cattcctatg actgtagatt ttatcagact gaagagctac tgtgtaagta
     9661 taattaactt ataattaaaa aaatagggcc attctagttt tatctatatt ttttttaaac
     9721 ttgtgcaaac tatgctacat aatcaactta cttgattatg taatatatat agtaatagtt
     9781 ggaattacta ttattacata tttatttttc tgtgctgggg tagaacccag ggaactaagt
     9841 actgtaataa ccgagcttca ttcatcctag tcatttttaa tttttttttt tttttttgtt
     9901 ttttcgagac aaggtttctc tgtgtagccc tggctgtcct ggaactcact ctgtagacca
     9961 ggctggcctc gaactcagaa atctgcctgc ctctgcctcc cagtgctgag attaatgacg
    10021 tgcaccacca ctgcctggca tttttttaaa gttttaattt gagacccttt ctcactaact
    10081 tgctcacact atcctcgaac tcattacgtg ggctggtcta gaaatttcag tcaacttgcc
    10141 tcagcttctc aagtagctgg gattacaggc aggtgctacc acttctggcc atgatcattg
    10201 ttgttgttgc tattattatt attattattt cactgatact tcatatcaca acacagaata
    10261 gtaactgtag ttcatgacca acaaaaaagt tgtacagttt ctctgttgtc ttattttgat
    10321 ctggattttg atttgtttca tggcactggg caggctaatc tcaaactgca tggctcaaat
    10381 agtccttctg tcttaatctc ccatgttctg gaagtatagg tatcactaca cctagctgta
    10441 ttttcatatt ttaaaaaaaa cgcctaagaa ttgctattga atgaggcgga gagatggctc
    10501 agcagtcaca agtacttgta gaggactggg gtttggttac tagcacctca aagatggctt
    10561 acaattatct gtagctccaa ttccagagga tctgtgtggt aaagctgcat gtatatgggc
    10621 atgcaggtaa aactgctaaa catataaaaa tagctcagtc ttaagagaat tggtcgacct
    10681 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacaat tcactggccg
    10741 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    10801 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    10861 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
    10921 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    10981 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    11041 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    11101 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    11161 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    11221 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    11281 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    11341 caatttaggt g
//
