LOCUS       sequence-228258-        7388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, 
            DeCaprio JA, Weinberg RA
  TITLE     Enumeration of the simian virus 40 early region elements necessary 
            for human cell transformation.
  JOURNAL   Mol Cell Biol 2002 Apr;22(7):2111-23.
  PUBMED    11884599
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-04"; volume: "22(7)"; pages: "2111-23"
COMMENT     Sequence Label: pBABE-zeo largeTgenomic
FEATURES             Location/Qualifiers
     source          1..7388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             9..478
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    543..742
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             943..1359
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1289..1311
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1327..1343
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(1446..1691,2038..3918)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     intron          1692..2037
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     primer_bind     complement(3994..4014)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3999..4328
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4179..4314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4338..4712
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             4883..5352
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5482..5499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5653..6241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5733..5752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6401..7192,7193..7261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7024..7043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7262..7366)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgcagcct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
       61 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      121 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      181 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      241 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      301 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      361 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      421 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
      481 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      541 gcaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
      601 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      661 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc
      721 cgtttttgtg gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat
      781 gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt
      841 tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt
      901 tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact
      961 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
     1021 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
     1081 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
     1141 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
     1201 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
     1261 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
     1321 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccg cggcctctga
     1381 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttg
     1441 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     1501 ttgaaaggag tgcctggggg aatattcctc tgatgagaaa ggcatattta aaaaaatgca
     1561 aggagtttca tcctgataaa ggaggagatg aagaaaaaat gaagaaaatg aatactctgt
     1621 acaagaaaat ggaagatgga gtaaaatatg ctcatcaacc tgactttgga ggcttctggg
     1681 atgcaactga ggtatttgct tcttccttaa atcctggtgt tgatgcaatg tactgcaaac
     1741 aatggcctga gtgtgcaaag aaaatgtctg ctaactgcat atgcttgctg tgcttactga
     1801 ggatgaagca tgaaaataga aaattataca ggaaagatcc acttgtgtgg gttgattgct
     1861 actgcttcga ttgctttaga atgtggtttg gacttgatct ttgtgaagga accttacttc
     1921 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     1981 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     2041 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     2101 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     2161 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     2221 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     2281 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     2341 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     2401 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     2461 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatcc attttctgtt
     2521 attgaggaaa gtttgccagg tgggttaaag gagcatgatt ttaatccaga agaagcagag
     2581 gaaactaaac aagtgtcctg gaagcttgta acagagtatg caatggaaac aaaatgtgat
     2641 gatgtgttgt tattgcttgg gatgtacttg gaatttcagt acagttttga aatgtgttta
     2701 aaatgtatta aaaaagaaca gcccagccac tataagtacc atgaaaagca ttatgcaaat
     2761 gctgctatat ttgctgacag caaaaaccaa aaaaccatat gccaacaggc tgttgatact
     2821 gttttagcta aaaagcgggt tgatagccta caattaacta gagaacaaat gttaacaaac
     2881 agatttaatg atcttttgga taggatggat ataatgtttg gttctacagg ctctgctgac
     2941 atagaagaat ggatggctgg agttgcttgg ctacactgtt tgttgcccaa aatggattca
     3001 gtggtgtatg actttttaaa atgcatggtg tacaacattc ctaaaaaaag atactggctg
     3061 tttaaaggac caattgatag tggtaaaact acattagcag ctgctttgct tgaattatgt
     3121 ggggggaaag ctttaaatgt taatttgccc ttggacaggc tgaactttga gctaggagta
     3181 gctattgacc agtttttagt agtttttgag gatgtaaagg gcactggagg ggagtccaga
     3241 gatttgcctt caggtcaggg aattaataac ctggacaatt taagggatta tttggatggc
     3301 agtgttaagg taaacttaga aaagaaacac ctaaataaaa gaactcaaat atttccccct
     3361 ggaatagtca ccatgaatga gtacagtgtg cctaaaacac tgcaggccag atttgtaaaa
     3421 caaatagatt ttaggcccaa agattattta aagcattgcc tggaacgcag tgagtttttg
     3481 ttagaaaaga gaataattca aagtggcatt gctttgcttc ttatgttaat ttggtacaga
     3541 cctgtggctg agtttgctca aagtattcag agcagaattg tggagtggaa agagagattg
     3601 gacaaagagt ttagtttgtc agtgtatcaa aaaatgaagt ttaatgtggc tatgggaatt
     3661 ggagttttag attggctaag aaacagtgat gatgatgatg aagacagcca ggaaaatgct
     3721 gataaaaatg aagatggtgg ggagaagaac atggaagact cagggcatga aacaggcatt
     3781 gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat
     3841 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3901 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt ggatcccagt gtggtggtac
     3961 gtaggaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa
     4021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4081 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4141 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4201 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4261 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4321 tttgcaaaaa gcttaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     4381 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     4441 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     4501 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     4561 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     4621 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     4681 gcgtgcactt cgtggccgag gagcaggact gaccgatcga taaaataaaa gattttattt
     4741 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     4801 gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca
     4861 aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     4921 tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata
     4981 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     5041 tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     5101 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     5161 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     5221 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     5281 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     5341 gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta
     5401 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6061 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccgca
     6361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7381 aagtgcca
//
