LOCUS       sequence-228287-       10051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable TCR expression in human T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Wang R, Pscheid R, Ghumra A, Kan LYL, Cochrane S, Fairclough L, 
            Alcocer MJC
  TITLE     Towards a surrogate system to express human lipid binding TCRs.
  JOURNAL   Biotechnol Lett. 2019 Oct;41(10):1095-1104. doi: 
            10.1007/s10529-019-02713-2. Epub 2019 Jul 26.
  PUBMED    31346817
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10529-019-02713-2"; journalName: "Biotechnol Lett"; date: 
            "2019-10"; volume: "41"; issue: "10"; pages: "1095-1104"
COMMENT     Sequence Label: pMJA285= pSin-TCRab-CMVp-Puro
FEATURES             Location/Qualifiers
     source          1..10051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1415..1648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1833..1877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2164..2281
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2429..2640
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        3888..4191
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4192..4395
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4346..4366
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(5597..5616)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5662..5717
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5716..5735)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(6167..6741,6742..6744,6745..6753)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6334..6351)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6561..6580
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6773..7372
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6773..6792)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7269..7289
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7575..7808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7959..7976)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7965..8189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      8232..8241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        8285..8389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8390..8458,8459..9250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8608..8627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9421..10009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9910..9929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2461 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2521 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2581 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2641 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2701 ccttgaatta cttccacctg gctgcagtaa tttaaaacta gatcagctgg accacagtct
     2761 cagggtcatc agcaggttga agccggccac cttcagcagc aggattctga agccgatcac
     2821 gctcaggttc tggaagttca ggttggtgtc tgtctcgaag ctcttttcca ccagcttcac
     2881 gtcgcagctg ctctcggggc ttgggaagaa tgtgtcctcg gggataatgc tgttgttgaa
     2941 ggcgttggcg caggcgaagt cgctcttgtt ggaccaggcc acggcgctgt tagacttgaa
     3001 gtccatggat ctcatgtcca gcacggtctt gtcggtgatg tacacgtcgc tgtccttgct
     3061 ctgggacacg ttggtctggc tgtcgaagtc ggtgaacagg cacacgctct tgtcgctgga
     3121 cttggagtct ctcagctggt acacggcagg gtcagggttc tggatgtcgg gccacacggt
     3181 cagctgggtt cctctgccga agtacagtct gcccagggtg ctgcctctgt cggacaccac
     3241 gcagatgtag ctggcgctgt cgctcagctg gctggcggtg atgtgcaggc tgctctgctt
     3301 tgtgtcggcg tccagggtgg cggtgtatct gccgttgctc ttggtgttct cgctgaaggt
     3361 catgatggtc agggacacgg ggcctctgcc ggtgtcctgc ttgtaccatc tcaggttgct
     3421 gaatggggac acggtgtagt tgcactgcag ggtgcagttc ttgccttcca ggatgatcag
     3481 gctctggggg ctctgttcca cctggttctt gccgttgcct ctgtagaagt acagccacag
     3541 gatcacgaga aaggtggtca ggtgcttctt catggttgtt ctggagaagc ctgcctcaaa
     3601 tctgctgcct tgtttcatca gctgtttctt gtaacattaa acagggactc tcaggaagta
     3661 cagtcagcca atcagagaac agatgtgcac aagcacacac acacacacac aggatctggg
     3721 cccccgcggc atatgaccgg tgaattctcc aggcgatctg acggttcact aaacgagctc
     3781 tgcttatata ggcctcccac cgtacacgcc acctcgacat actcgagtag ttattaatag
     3841 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3901 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3961 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     4021 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     4081 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     4141 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     4201 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4261 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4321 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4381 tatataagca gagctggttt agtgaaccgt cagatccgct agggatcctc tagtcagctg
     4441 acgcgtgcta gcgcggccgc atcgataagc ttgtcgagca gagcctatta ggagactaca
     4501 gatccaaacc tccaaatagt tcccccagaa gtcctggacc ctgaacaagg ggatgctgtc
     4561 atgaccatca gactgctgtg ctacgtgggc ttctacttcc tgggcgctgg cctgatggaa
     4621 gccgacatct accagacccc cagatacctc gtgatcggca ccggcaagaa aatcaccctg
     4681 gaatgcagcc agaccatggg ccacgacaag atgtactggt atcagcagga ccccggcatg
     4741 gaactgcacc tgatccacta cagctacggc gtgaacagca ccgagaaggg cgacctgagc
     4801 agcgagagca ccgtgtccag aatcagaacc gagcacttcc cactgaccct ggaaagcgct
     4861 agacccagcc acaccagcca gtacctgtgt gccagcagct ctcctctggg cggaacacct
     4921 ggcgccaacg tgctgacatt tggcgctggc agcagactga ccgtgctgga agatctgaag
     4981 aacgtgttcc cacccgaggt ggccgtgttc gagccttctg aggccgagat ctcccacacc
     5041 cagaaagcca ccctcgtgtg tctggccacc ggcttctacc ccgaccacgt ggaactgtct
     5101 tggtgggtca acggcaaaga ggtgcacagc ggcgtgtcca ccgatcccca gcctctgaaa
     5161 gagcagcccg ccctgaacga cagcagatac tgcctgtcca gcaggctgag agtgtccgcc
     5221 accttctggc agaaccccag aaaccacttc agatgccagg tgcagtttta cggcctgagc
     5281 gagaacgacg agtggaccca ggacagagcc aagcccgtga cccagatcgt gtctgctgag
     5341 gcctggggca gagccgactg tggcttcaca agcgagagct accagcaggg cgtgctgagc
     5401 gccaccatcc tgtacgagat cctgctgggc aaggccaccc tgtacgccgt gctggtgtct
     5461 gccctggtgc tgatggccat ggtcaagaga aaggacagca gaggctgaga attcaattca
     5521 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     5581 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     5641 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     5701 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     5761 atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg
     5821 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     5881 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     5941 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     6001 ctactcccag tcatagctgt ccctcttctc ttactagtaa tttcctaggt agtttaaaca
     6061 agaaactttt gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagttcgaag
     6121 gatccgcatg catctagggc ggccaattcc gcccctctcc cccccccccc tctccctccc
     6181 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     6241 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     6301 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     6361 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     6421 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     6481 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     6541 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     6601 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6661 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6721 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     6781 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6841 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6901 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6961 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7021 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7081 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7141 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7201 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7261 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7321 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gataataggc
     7381 ggccgctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     7441 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     7501 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7801 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7861 aatatcagag agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag
     7921 tggagcacac aggcaaagtt ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     7981 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8041 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8101 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8161 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctgggggcg
     8221 cgcccctcga ggccgccatg gtcatagctg tttgacgtca ggtggcactt ttcggggaaa
     8281 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcac
     8341 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8401 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8461 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8521 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8581 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8641 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8701 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8761 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8821 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8881 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8941 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9001 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9061 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9121 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9181 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9241 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9301 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9361 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10021 cgcggccttt ttacggttct ggccttttgc a
//
