LOCUS       sequence-228291-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BioID2 and use
            in proximity-dependent biotin identification (BioID); MCS is present
            upstream of BioID2 tag and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  TITLE     Unpublished BioID and BioID2 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MCS-BioID2-HA pBabe-puro
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
ORIGIN
        1 ttcaagaacc tgatctggct gaaggaggtg gacagcaccc aggagagact gaaggagtgg
       61 aacgtgagct acggcaccgc cctggtggcc gacagacaga ccaagggcag aggcggcctg
      121 ggcagaaagt ggctgagcca ggagggcggc ctgtacttca gcttcctgct gaaccccaag
      181 gagttcgaga acctgctgca gctgcccctg gtgctgggcc tgagcgtgag cgaggccctg
      241 gaggagatca ccgagatccc cttcagcctg aagtggccca acgacgtgta cttccaggag
      301 aagaaggtga gcggcgtgct gtgcgagctg agcaaggaca agctgatcgt gggcatcggc
      361 atcaacgtga accagagaga gatccccgag gagatcaagg acagagccac caccctgtac
      421 gagatcaccg gcaaggactg ggacagaaag gaggtgctgc tgaaggtgct gaagagaatc
      481 agcgagaacc tgaagaagtt caaggagaag agcttcaagg agttcaaggg caagatcgag
      541 agcaagatgc tgtacctggg cgaggaggtg aagctgctgg gcgagggcaa gatcaccggc
      601 aagctggtgg gcctgagcga gaagggcggc gccctgatcc tgaccgagga gggcatcaag
      661 gagatcctga gcggcgagtt cagcctgaga agaagc
//
