LOCUS       sequence-228319-       11407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inserts mCherry immediately downstream of the Nanog coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Faddah DA, Wang H, Cheng AW, Katz Y, Buganim Y, Jaenisch R
  TITLE     Single-cell analysis reveals that expression of nanog is biallelic 
            and equally variable as that of other pluripotency factors in mouse 
            ESCs.
  JOURNAL   Cell Stem Cell. 2013 Jul 3;13(1):23-9. doi: 
            10.1016/j.stem.2013.04.019.
  PUBMED    23827708
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.04"; journalName: "Cell Stem Cell"; date: 
            "2013-07-3- 3"; volume: "13"; issue: "1"; pages: "23-9"
COMMENT     Sequence Label: Nanog-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..11407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..68
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    185..206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(221..226,227..244,245..251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    259..275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     264..286
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2388..2444
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2445..3155
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2590..2608)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2858..2877
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    3188..3221
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3248..3271)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3645..3664
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3726..4529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4561..4578)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4567..4678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4766..4799
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7918..7937)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7919..7937)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7944..7961)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7944..7960)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7953..7975)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8101..8529
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8188..8207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8398..8419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             8873..9667
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(9685..9753,9754..10545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9903..9922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10716..11304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11205..11224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
       61 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
      121 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      181 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      241 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      301 atgattacgc caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg
      361 gaattcgccc ttttcctgca ggctctgaag acctgcctct tcaaggcagc cctgattctt
      421 ctaccagtcc caaacaaaag ctctcaagtc ctgaggctga caagggccct gaggaggagg
      481 agaacaaggt ccttgccagg aagcagaaga tgcggactgt gttctctcag gcccagctgt
      541 gtgcactcaa ggacaggttt cagaagcaga agtacctcag cctccagcag atgcaagaac
      601 tctcctccat tctgaacctg agctataagc aggtggggtc cttagaagtt gctgtaatgt
      661 aacacaatga gactgacttg ctaatgaatc gaactaacgt ctggacgtct ctctaactat
      721 cctatcctcc tatcctatgc gcctgttaaa atcataatta tgtgattgat tgattgattg
      781 attatgtata agtacactat agctgtcttc agatacacca gaagagggca tcagatctca
      841 ttacggatgg ttgtgagcca ccatgtggtt gctgggattt gaactcagga ccttcggaag
      901 agcagtcagt gctcttaacc gctgagccat ctctccagcc ccaattatgt gatttatata
      961 tttcccagtt tgtttcattg gttactttat cttctacttc acctcccaca cccatcccca
     1021 aatcagggtc tcactgtagc cctgactggt ctctgtagac caggctggga gaggttcacc
     1081 tgcctctgtc tcctgggtgc tggaaataaa ggtgtgcctg gcttttttcc tttcttttct
     1141 tttttaagat tgtttatttt taaattcctt tagttcattg gtgttttgcc tgcatgtgtt
     1201 tgtctttgtg agagtatcag atctcctgga ctagagttac agacagtcgt gagctgccat
     1261 gtggccgctg agaattgaac ccaggtcctc tggaagagca gccagtgctc ttaacagaag
     1321 agccatttcc cccagcacct gtctttttca agaaagagtt tcactttaca ggcctggctg
     1381 tcatgtttaa ggtcgggctg tagacctgtc tgcattctgt atgtcttagc ttgttcgaat
     1441 gtcctgtcac tctgcaggtt aagacctggt ttcaaaacca aaggatgaag tgcaagcggt
     1501 ggcagaaaaa ccagtggttg aagactagca atggtctgat tcaggtaaca agatgttttc
     1561 tctgctttcc tttttccccc tccatcttac catcttattt tatgtgtctg tgccgtgtgt
     1621 gtgtgtgtgt gtgtgtgtgt ggctgaccta tggctgttgg gtatggatcc cagatttcct
     1681 gtaagagcag tcagtggctc tgcctttgct tcccagtgtc atcctgatcc atctatgtcc
     1741 attagtcctg tccctttccc atccctcttc ctccacgcac tctttcttgt gatctttggt
     1801 tctttgaggc aggctctctc tatgtagccc tggcagtcag agatctgcct gcctagtctc
     1861 aggagtgctg gggttaacgg cctgtgcggc catgctaatc ttttcagtat ttctgcagtt
     1921 tacatcttta gagtcccctt ttcactcatt gcattaatca cctctttcag aagggctcag
     1981 caccagtgga gtatcccagc atccattgca gctatcccca gggctatctg gtgaacgcat
     2041 ctggaagcct ttccatgtgg ggcagccaga cttggaccaa cccaacttgg agcagccaga
     2101 cctggaccaa cccaacttgg aacaaccaga cctggaccaa cccaacttgg agcagccagg
     2161 cctggaccgc tcagtcctgg aacggccagc cttggaatgc tgctccgctc cataacttcg
     2221 gggaggactt tctgcagcct tacgtacagt tgcagcaaaa cttctctgcc agtgatttgg
     2281 aggtgaattt ggaagccact agggaaagcc atgcgcattt tagcacccca caagccttgg
     2341 aattattcct gaactactct gtgactccac caggtgaaat agctagcgcc actaacttct
     2401 ccctgttgaa acaagcaggg gatgtcgaag agaatcccgg gccaatggtg agcaagggcg
     2461 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     2521 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2581 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2641 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2701 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2761 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2821 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2881 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2941 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3001 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     3061 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     3121 actccaccgg cggcatggac gagctgtaca agtaattaat taaccagtgt gctggaattc
     3181 ggcttcaaca acttcgtata atgtatgcta tacgaagtta tcagtacttt tcccaaggca
     3241 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     3301 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     3361 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     3421 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     3481 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     3541 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     3601 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     3661 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     3721 ccaatatggg atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3781 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3841 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3901 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3961 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4021 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4081 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4141 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4201 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4261 cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4321 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4381 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4441 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4501 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag
     4561 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4621 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4681 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4741 aggattggga agacaatagc aggcaacaac ttcgtataat gtatgctata cgaagttatc
     4801 agtacaagcc gaattctgca gatatccatc ggcgcgccga cttacgcaac atctgggctt
     4861 aaagtcaggg caaagccagg ttccttcctt cttccaaata ttttcatatt ttttttaaag
     4921 atttatttat tcattatatg taagtacact gtagctgtct tcagacactc cagaagaggg
     4981 cgtcagatct tgttacgtat ggttgtgagc caccatgtgg ttgctgggat ttgaactcct
     5041 gaccttcgga agagcagtcg ggtgctctta tccactgagc catctcacca gcccctggtt
     5101 tattttttta attattattt gctttttgtt tatcaagaca gggtttctct gcatagctct
     5161 aattgtcttt gaactagctc tgcagaccag cctggccttg aactcagaga tctgcccact
     5221 tatctttgcc tcctgaatgc tgggaccaaa ggtggcatac caccacacct ggcatatata
     5281 ttgtttattt ctatttctat ttttattggt gccagagcaa acctaggact tagaacatgc
     5341 tgggcaccaa ctcaacttct gagctctatt tacaacttgg tgtgttagtg tatttgtctt
     5401 agttctgaat ttgtcctttt tttagtgtta actctaggct ttggagacag tgaggtgcat
     5461 atactctctc cttcccaaga ataagtgctt gaacaccctt acccacgccc acccacccat
     5521 gctagtcttt tttcttagaa gcgtgggtct tggtatacac tgtgtcattt tgaggggtga
     5581 ggtttaaaag tatatacaaa gtataacgat atggtggcta ctctcgagga tgagacagaa
     5641 ggaccaggag tttgagggta gctcagatat gcaataagtt caaggccaac ctgtactatg
     5701 tttaaatagt aagacagcat ctcgataaaa taataaaact aaagtctcaa caaaataaaa
     5761 gctttcacct attaaggtgc ttgcttgtcc ttggagtccc ccaagagtaa ctgctatgtt
     5821 aatatctgta gaaagatgtt tatatttgac tgtaccatga tgaaccgatg ccagctggac
     5881 tagtttaaac aaaataaaac actaatttta cctttagtgg tcttatcttt tgtccagtgg
     5941 ggtcaatata ctttttattt tggtatgtga aggggagggt cttaaccgag ggtccaggaa
     6001 aatgggtttc ttttggctta accggttggg agggttatac cacaacagtt ggaatggcct
     6061 ggtaaccaga gcaggaggcc agccactcac aatgcatcca gtcaagaact ggagagcaat
     6121 gaataagaaa tgaatgtttt agtgtgggta tgggccctga ggaggccaga agcattcgat
     6181 gatcccctgg agatgaagtt acgtgaagcc acctgatcac ctgatgtggc aggctgcagg
     6241 gaaataagct ctagttctct ggaagaacaa atatttaact gggagagtgg tttttattcc
     6301 tgtgaaataa tagaacttgt ggagagagcc cctgaggtca cacacctagt gcttctggga
     6361 aagaggaggg accacagtct cttgtttttg tgtcacactg gagattaatc tgggacttcc
     6421 tggctccaga tggaggctat cttccctgag cataaccccc taacctacgt gggggtgggg
     6481 gaagatgtca agtgttatca caggagactt gagaggctgt gtgggttcag aagtgagttt
     6541 ggggttttgg agacgattta aatttgctga aaagacagaa gatacgtata catctggtgt
     6601 agttgtgtgt acacttaagg agattaagtt aacgtaactc ctgatgcatt aattatagat
     6661 gttatagtag ttgtatacag tagatttagc taaagcatga tacggaaaaa cacagaagaa
     6721 gcaagcgaca cctagtaact gccgtgcgta aatagccata cctgtcaatc acatctttaa
     6781 ctcctgacca ctcacagggg tacccagccc agcccaagta ggttctgtag ctgctcctca
     6841 gctaaggaga gggctgtttt ggcagtgaga aaaattgtcc ctcattgtgc cacagaggct
     6901 aggacacatt cccttgcaaa gcccagacta attccaccta gaattctcct cttctgcttt
     6961 ctgtcctttg gcatcagtgg ggatggctgc tgtgtcagag atgggacatc atcacctcca
     7021 caaaccctga cgaccgttac tgagagacca ctttgcttca actcttagag gtttcgttga
     7081 taagattcta aaatgtgtgg tcttggggat caaatccagg gactcttaga ccctgggtaa
     7141 gtgctctact actgaacaac actcagtcct tggtgtgaga atccgagctt tgattttacc
     7201 ctttttaggg tggaagagat tccaccaatc acccaggagt ggggctagtt caagaggcct
     7261 ccccagctgc agctttcaga ggctttttgc ttcaggctcc caccaaggga cctattcaaa
     7321 tgccttagtc aactgacatc tgagtcccaa gctgttgcag gaccccagct gacttcttta
     7381 tttaacaaat cctccttttt cccctgctcg ggctcctctc cctcctctgt ctggcaactg
     7441 tccgctttct ctttgtagtt ccccaccagg acctgttttc tctccactgt caccaggttt
     7501 tctgccgact gggtttattc acaacagaat taaccttttc ctttacccag agaagccagc
     7561 ccttggaatt caatggcaga gaaggtctgt gcttctgagc ttgtagtttg cactgtccaa
     7621 ttccgaagac ccaggcaaat ttgatccagg gtgctccgtg ttagacctga ggaagaaacc
     7681 aattccaaga ctgcctgatt aaagcaggtt ttacttagag gaacagcagg aactggccag
     7741 cccatgcaca tgagtaaaac aagcttggtt ggattaattt ggccacgatg gtttgcgtta
     7801 gtggtcttcc ttctcccaac tttgggattg acatttgcgg ccggccaaaa gggcgaattc
     7861 tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc
     7921 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7981 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     8041 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8101 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8161 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8221 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8281 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8341 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8401 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8461 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8521 acgcgaattt taacaaaatt cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
     8581 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
     8641 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
     8701 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
     8761 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
     8821 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     8881 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     8941 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     9001 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     9061 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     9121 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     9181 gtcatcccac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     9241 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     9301 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     9361 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     9421 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     9481 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     9541 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     9601 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     9661 cttctgaatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9781 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9841 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9901 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9961 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10021 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10081 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10141 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10201 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10261 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10321 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10381 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10441 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10501 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10561 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10621 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10681 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10741 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10801 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10861 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10921 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10981 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11041 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11101 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11161 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11221 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11281 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    11341 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    11401 cgtatta
//
