LOCUS       sequence-228335-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 
            {AT1G77120}(pEPOR1CB0029).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0038
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4573
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4574..4621
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4628..4708
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      4724..4976
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5323..5347
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5656..6285
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6714..7787
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7853..8047
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        8109..8213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8214..9008
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9179..9767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9668..9687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9903..9927
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tgagcaagct
      901 ggagaagttt acaaactgct actccctgtc taagaccctg aggttcaagg ccatccctgt
      961 gggcaagacc caggagaaca tcgacaataa gcggctgctg gtggaggacg agaagagagc
     1021 cgaggattat aagggcgtga agaagctgct ggatcgctac tatctgtctt ttatcaacga
     1081 cgtgctgcac agcatcaagc tgaagaatct gaacaattac atcagcctgt tccggaagaa
     1141 aaccagaacc gagaaggaga ataaggagct ggagaacctg gagatcaatc tgcggaagga
     1201 gatcgccaag gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga aggatatcat
     1261 cgagacaatc ctgccagagt tcctggacga taaggacgag atcgccctgg tgaacagctt
     1321 caatggcttt accacagcct tcaccggctt ctttgataac agagagaata tgttttccga
     1381 ggaggccaag agcacatcca tcgccttcag gtgtatcaac gagaatctga cccgctacat
     1441 ctctaatatg gacatcttcg agaaggtgga cgccatcttt gataagcacg aggtgcagga
     1501 gatcaaggag aagatcctga acagcgacta tgatgtggag gatttctttg agggcgagtt
     1561 ctttaacttt gtgctgacac aggagggcat cgacgtgtat aacgccatca tcggcggctt
     1621 cgtgaccgag agcggcgaga agatcaaggg cctgaacgag tacatcaacc tgtataatca
     1681 gaaaaccaag cagaagctgc ctaagtttaa gccactgtat aagcaggtgc tgagcgatcg
     1741 ggagtctctg agcttctacg gcgagggcta tacatccgat gaggaggtgc tggaggtgtt
     1801 tagaaacacc ctgaacaaga acagcgagat cttcagctcc atcaagaagc tggagaagct
     1861 gttcaagaat tttgacgagt actctagcgc cggcatcttt gtgaagaacg gccccgccat
     1921 cagcacaatc tccaaggata tcttcggcga gtggaacgtg atccgggaca agtggaatgc
     1981 cgagtatgac gatatccacc tgaagaagaa ggccgtggtg accgagaagt acgaggacga
     2041 tcggagaaag tccttcaaga agatcggctc cttttctctg gagcagctgc aggagtacgc
     2101 cgacgccgat ctgtctgtgg tggagaagct gaaggagatc atcatccaga aggtggatga
     2161 gatctacaag gtgtatggct cctctgagaa gctgttcgac gccgattttg tgctggagaa
     2221 gagcctgaag aagaacgacg ccgtggtggc catcatgaag gacctgctgg attctgtgaa
     2281 gagcttcgag aattacatca aggccttctt tggcgagggc aaggagacaa acagggacga
     2341 gtccttctat ggcgattttg tgctggccta cgacatcctg ctgaaggtgg accacatcta
     2401 cgatgccatc cgcaattatg tgacccagaa gccctactct aaggataagt tcaagctgta
     2461 ttttcagaac cctcagttca tgggcggctg ggacaaggat aaggagacag actatcgggc
     2521 caccatcctg agatacggct ccaagtacta tctggccatc atggataaga agtacgccaa
     2581 gtgcctgcag aagatcgaca aggacgatgt gaacggcaat tacgagaaga tcaactataa
     2641 gctgctgccc ggccctaata agatgctgcc aaaggtgttc ttttctaaga agtggatggc
     2701 ctactataac cccagcgagg acatccagaa gatctacaag aatggcacat tcaagaaggg
     2761 cgatatgttt aacctgaatg actgtcacaa gctgatcgac ttctttaagg atagcatctc
     2821 ccggtatcca aagtggtcca atgcctacga tttcaacttt tctgagacag agaagtataa
     2881 ggacatcgcc ggcttttaca gagaggtgga ggagcagggc tataaggtga gcttcgagtc
     2941 tgccagcaag aaggaggtgg ataagctggt ggaggagggc aagctgtata tgttccagat
     3001 ctataacaag gacttttccg ataagtctca cggcacaccc aatctgcaca ccatgtactt
     3061 caagctgctg tttgacgaga acaatcacgg acagatcagg ctgagcggag gagcagagct
     3121 gttcatgagg cgcgcctccc tgaagaagga ggagctggtg gtgcacccag ccaactcccc
     3181 tatcgccaac aagaatccag ataatcccaa gaaaaccaca accctgtcct acgacgtgta
     3241 taaggataag aggttttctg aggaccagta cgagctgcac atcccaatcg ccatcaataa
     3301 gtgccccaag aacatcttca agatcaatac agaggtgcgc gtgctgctga agcacgacga
     3361 taacccctat gtgatcggca tcgatagggg cgagcgcaat ctgctgtata tcgtggtggt
     3421 ggacggcaag ggcaacatcg tggagcagta ttccctgaac gagatcatca acaacttcaa
     3481 cggcatcagg atcaagacag attaccactc tctgctggac aagaaggaga aggagaggtt
     3541 cgaggcccgc cagaactgga cctccatcga gaatatcaag gagctgaagg ccggctatat
     3601 ctctcaggtg gtgcacaaga tctgcgagct ggtggagaag tacgatgccg tgatcgccct
     3661 ggaggacctg aactctggct ttaagaatag ccgcgtgaag gtggagaagc aggtgtatca
     3721 gaagttcgag aagatgctga tcgataagct gaactacatg gtggacaaga agtctaatcc
     3781 ttgtgcaaca ggcggcgccc tgaagggcta tcagatcacc aataagttcg agagctttaa
     3841 gtccatgtct acccagaacg gcttcatctt ttacatccct gcctggctga catccaagat
     3901 cgatccatct accggctttg tgaacctgct gaaaaccaag tataccagca tcgccgattc
     3961 caagaagttc atcagctcct ttgacaggat catgtacgtg cccgaggagg atctgttcga
     4021 gtttgccctg gactataaga acttctctcg cacagacgcc gattacatca agaagtggaa
     4081 gctgtactcc tacggcaacc ggatcagaat cttccggaat cctaagaaga acaacgtgtt
     4141 cgactgggag gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca agtacggcat
     4201 caattatcag cagggcgata tcagagccct gctgtgcgag cagtccgaca aggccttcta
     4261 ctctagcttt atggccctga tgagcctgat gctgcagatg cggaacagca tcacaggccg
     4321 caccgacgtg gattttctga tcagccctgt gaagaactcc gacggcatct tctacgatag
     4381 ccggaactat gaggcccagg agaatgccat cctgccaaag aacgccgacg ccaatggcgc
     4441 ctataacatc gccagaaagg tgctgtgggc catcggccag ttcaagaagg ccgaggacga
     4501 gaagctggat aaggtgaaga tcgccatctc taacaaggag tggctggagt acgcccagac
     4561 cagcgtgaag cacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa
     4621 gggatcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc
     4681 atacccatat gatgtccccg actatgccta agcttgtcaa gcagatcgtt caaacatttg
     4741 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4801 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4861 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4921 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
     4981 ctactagaat tcgagctcgg agcatcttca ttcttaagat atgaagataa tcttcaaaag
     5041 gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat
     5101 ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat cgcttagata
     5161 agaaaacgaa gctgagttta tatacagcta gagtcgaagt agtgattgta atttctacta
     5221 agtgtagata cgtatcttcg gccatgaagc tgtttttttc gctttacgag gatgcacatg
     5281 tgaccgaggg acacgaagtg atccgtttaa actatcagtg tttgacagga tatattggcg
     5341 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     5401 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtc
     5461 agccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     5521 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     5581 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     5641 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     5701 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     5761 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     5821 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     5881 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     5941 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     6001 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     6061 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     6121 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     6181 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     6241 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     6301 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     6361 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     6421 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     6481 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     6541 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     6601 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     6661 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     6721 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     6781 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     6841 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     6901 gtgcgtcgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     6961 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     7021 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     7081 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     7141 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     7201 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     7261 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     7321 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     7381 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     7441 tcgagctagc tgattggatg taccgcgaga ttacagaagg caagaacccg gacgtgctga
     7501 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     7561 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     7621 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     7681 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     7741 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     7801 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aagcttcttt cctgtggata
     7861 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     7921 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     7981 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     8041 gtgcataact gtctggccag cgcacagccg aacagctgca aaaagcgccc acatcaaggc
     8101 tccgagtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8161 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatggcta
     8221 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     8281 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     8341 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     8401 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     8461 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     8521 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     8581 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     8641 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaggaca
     8701 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     8761 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     8821 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     8881 tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     8941 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     9001 tgttttagct gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9781 cggccttttt acggttcctg ctcggatctg ttggaccgga cagtagtcat ggttgatggg
     9841 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     9901 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     9961 cggacgtttt taatgtactg gggtt
//
