LOCUS       sequence-228336-       10221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 
            {AT1G77120}(pEPOR1CB0028).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0039
FEATURES             Location/Qualifiers
     source          1..10221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4810
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4530..4539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4811..4858
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4865..4945
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      4961..5213
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5559..5583
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5892..6521
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6950..8023
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8089..8283
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        8345..8449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8450..9244
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9415..10003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9904..9923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10139..10163
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tgacacagtt
      901 cgagggcttt accaacctgt atcaggtgag caagacactg cggtttgagc tgatcccaca
      961 gggcaagacc ctgaagcaca tccaggagca gggcttcatc gaggaggaca aggcccgcaa
     1021 tgatcactac aaggagctga agcccatcat cgatcggatc tacaagacct atgccgacca
     1081 gtgcctgcag ctggtgcagc tggattggga gaacctgagc gccgccatcg actcctatag
     1141 aaaggagaaa accgaggaga caaggaacgc cctgatcgag gagcaggcca catatcgcaa
     1201 tgccatccac gactacttca tcggccggac agacaacctg accgatgcca tcaataagag
     1261 acacgccgag atctacaagg gcctgttcaa ggccgagctg tttaatggca aggtgctgaa
     1321 gcagctgggc accgtgacca caaccgagca cgagaacgcc ctgctgcgga gcttcgacaa
     1381 gtttacaacc tacttctccg gcttttatga gaacaggaag aacgtgttca gcgccgagga
     1441 tatcagcaca gccatcccac accgcatcgt gcaggacaac ttccccaagt ttaaggagaa
     1501 ttgtcacatc ttcacacgcc tgatcaccgc cgtgcccagc ctgcgggagc actttgagaa
     1561 cgtgaagaag gccatcggca tcttcgtgag cacctccatc gaggaggtgt tttccttccc
     1621 tttttataac cagctgctga cacagaccca gatcgacctg tataaccagc tgctgggagg
     1681 aatctctcgg gaggcaggca ccgagaagat caagggcctg aacgaggtgc tgaatctggc
     1741 catccagaag aatgatgaga cagcccacat catcgcctcc ctgccacaca gattcatccc
     1801 cctgtttaag cagatcctgt ccgataggaa caccctgtct ttcatcctgg aggagtttaa
     1861 gagcgacgag gaagtgatcc agtccttctg caagtacaag acactgctga gaaacgagaa
     1921 cgtgctggag acagccgagg ccctgtttaa cgagctgaac agcatcgacc tgacacacat
     1981 cttcatcagc cacaagaagc tggagacaat cagcagcgcc ctgtgcgacc actgggatac
     2041 actgaggaat gccctgtatg agcggagaat ctccgagctg acaggcaaga tcaccaagtc
     2101 tgccaaggag aaggtgcagc gcagcctgaa gcacgaggat atcaacctgc aggagatcat
     2161 ctctgccgca ggcaaggagc tgagcgaggc cttcaagcag aaaaccagcg agatcctgtc
     2221 ccacgcacac gccgccctgg atcagccact gcctacaacc ctgaagaagc aggaggagaa
     2281 ggagatcctg aagtctcagc tggacagcct gctgggcctg taccacctgc tggactggtt
     2341 tgccgtggat gagtccaacg aggtggaccc cgagttctct gcccggctga ccggcatcaa
     2401 gctggagatg gagccttctc tgagcttcta caacaaggcc agaaattatg ccaccaagaa
     2461 gccctactcc gtggagaagt tcaagctgaa ctttcagatg cctacactgg cctctggctg
     2521 ggacgtgaat aaggagaaga acaatggcgc catcctgttt gtgaagaacg gcctgtacta
     2581 tctgggcatc atgccaaagc agaagggcag gtataaggcc ctgagcttcg agcccacaga
     2641 gaaaaccagc gagggctttg ataagatgta ctatgactac ttccctgatg ccgccaagat
     2701 gatcccaaag tgcagcaccc agctgaaggc cgtgacagcc cactttcaga cccacacaac
     2761 ccccatcctg ctgtccaaca atttcatcga gcctctggag atcacaaagg agatctacga
     2821 cctgaacaat cctgagaagg agccaaagaa gtttcagaca gcctacgcca agaaaaccgg
     2881 cgaccagaag ggctacagag aggccctgtg caagtggatc gacttcacaa gggattttct
     2941 gtccaagtat accaagacaa cctctatcga tctgtctagc ctgcggccat cctctcagta
     3001 taaggacctg ggcgagtact atgccgagct gaatcccctg ctgtaccaca tcagcttcca
     3061 gagaatcgcc gagaaggaga tcatggatgc cgtggagaca ggcaagctgt acctgttcca
     3121 gatctataac aaggactttg ccaagggcca ccacggcaag cctaatctgc acacactgta
     3181 ttggaccggc ctgttttctc cagagaacct ggccaagaca agcatcaagc tgaatggcca
     3241 ggccgagctg ttctaccgcc ctaagtccag gatgaagagg atggcacacc ggctgggaga
     3301 gaagatgctg aacaagaagc tgaaggatca gaaaacccca atccccgaca ccctgtacca
     3361 ggagctgtac gactatgtga atcacagact gtcccacgac ctgtctgatg aggccagggc
     3421 cctgctgccc aacgtgatca ccaaggaggt gtctcacgag atcatcaagg ataggcgctt
     3481 taccagcgac aagttctttt tccacgtgcc tatcacactg aactatcagg ccgccaattc
     3541 cccatctaag ttcaaccaga gggtgaatgc ctacctgaag gagcaccccg agacacctat
     3601 catcggcatc gatcggggcg agagaaacct gatctatatc acagtgatcg actccaccgg
     3661 caagatcctg gagcagcgga gcctgaacac catccagcag tttgattacc agaagaagct
     3721 ggacaacagg gagaaggaga gggtggcagc aaggcaggcc tggtctgtgg tgggcacaat
     3781 caaggatctg aagcagggct atctgagcca ggtcatccac gagatcgtgg acctgatgat
     3841 ccactaccag gccgtggtgg tgctggagaa cctgaatttc ggctttaaga gcaagaggac
     3901 cggcatcgcc gagaaggccg tgtaccagca gttcgagaag atgctgatcg ataagctgaa
     3961 ttgcctggtg ctgaaggact atccagcaga gaaagtggga ggcgtgctga acccatacca
     4021 gctgacagac cagttcacct cctttgccaa gatgggcacc cagtctggct tcctgtttta
     4081 cgtgcctgcc ccatatacat ctaagatcga tcccctgacc ggcttcgtgg accccttcgt
     4141 gtggaaaacc atcaagaatc acgagagccg caagcacttc ctggagggct tcgactttct
     4201 gcactacgac gtgaaaaccg gcgacttcat cctgcacttt aagatgaaca gaaatctgtc
     4261 cttccagagg ggcctgcccg gctttatgcc tgcatgggat atcgtgttcg agaagaacga
     4321 gacacagttt gacgccaagg gcaccccttt catcgccggc aagagaatcg tgccagtgat
     4381 cgagaatcac agattcaccg gcagataccg ggacctgtat cctgccaacg agctgatcgc
     4441 cctgctggag gagaagggca tcgtgttcag ggatggctcc aacatcctgc caaagctgct
     4501 ggagaatgac gattctcacg ccatcgacac catggtggcc ctgatccgca gcgtgctgca
     4561 gatgcggaac tccaatgccg ccacaggcga ggactatatc aacagccccg tgcgcgatct
     4621 gaatggcgtg tgcttcgact cccggtttca gaacccagag tggcccatgg acgccgatgc
     4681 caatggcgcc taccacatcg ccctgaaggg ccagctgctg ctgaatcacc tgaaggagag
     4741 caaggatctg aagctgcaga acggcatctc caatcaggac tggctggcct acatccagga
     4801 gctgcgcaac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg
     4861 atcctaccca tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata
     4921 cccatatgat gtccccgact atgcctaagc ttgtcaagca gatcgttcaa acatttggca
     4981 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     5041 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     5101 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     5161 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgacgcta
     5221 ctagaattcg agctcggagc atcttcattc ttaagatatg aagataatct tcaaaaggcc
     5281 cctgggaatc tgaaagaaga gaagcaggcc catttatatg ggaaagaaca atagtatttc
     5341 ttatataggc ccatttaagt tgaaaacaat cttcaaaagt cccacatcgc ttagataaga
     5401 aaacgaagct gagtttatat acagctagag tcgaagtagt gattgtaatt tctactcttg
     5461 tagatacgta tcttcggcca tgaagctgtt tttttcgctt tacgaggatg cacatgtgac
     5521 cgagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta
     5581 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     5641 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggagtcagcc
     5701 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
     5761 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
     5821 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
     5881 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
     5941 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
     6001 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
     6061 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
     6121 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
     6181 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
     6241 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
     6301 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
     6361 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
     6421 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
     6481 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
     6541 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
     6601 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
     6661 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
     6721 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
     6781 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
     6841 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
     6901 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
     6961 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     7021 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     7081 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     7141 gtcgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     7201 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     7261 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     7321 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     7381 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     7441 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     7501 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     7561 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     7621 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     7681 gctagctgat tggatgtacc gcgagattac agaaggcaag aacccggacg tgctgacggt
     7741 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     7801 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     7861 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     7921 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     7981 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     8041 agggcaaatt gccctagcag gggaaaaagg tcgaaaaagc ttctttcctg tggatagcac
     8101 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     8161 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     8221 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     8281 ataactgtct ggccagcgca cagccgaaca gctgcaaaaa gcgcccacat caaggctccg
     8341 agtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8401 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat
     8461 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     8521 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     8581 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     8641 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     8701 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     8761 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     8821 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     8881 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc
     8941 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     9001 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     9061 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     9121 cgtccggtcg ctcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     9181 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     9241 ttagctgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10021 ctttttacgg ttcctgctcg gatctgttgg accggacagt agtcatggtt gatgggctgc
    10081 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    10141 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    10201 cgtttttaat gtactggggt t
//
