LOCUS       sequence-228342-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6 driven Spy sgRNA cloning vector where guide sequences are 
            inserted between BspMI sites. This plasmid works with Addgene 
            #108570 for subnuclear proteomic profiling via C-BERST method..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Gao XD, Rodriguez TC, Sontheimer EJ
  TITLE     Adapting dCas9-APEX2 for subnuclear proteomic profiling.
  JOURNAL   Methods Enzymol. 2019;616:365-383. doi: 10.1016/bs.mie.2018.10.030. 
            Epub 2018 Dec 10.
  PUBMED    30691651
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2019"; volume: "616:365-383. doi"; pages: " 
            10.1016/bs.mie.2018.10.030. Epub 2018 Dec 10"
COMMENT     Sequence Label: pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1833..2081
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1833..1853
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2004..2023
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(2212..3006)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2323..2342)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2933..2952
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    3299..3416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3465..3975
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3894..3912
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3984..4556
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4564..4583
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        4611..4629
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4640..5260
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4713..4732)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             5294..5350
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5366..6076
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGIYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6077..6097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             6193..6426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6498..6619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6535..6554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6589..6608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6659..6794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6815..6833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6843..6860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6843..6859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6852..6874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7001..7456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7088..7107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7298..7319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7482..7586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7587..7655,7656..8447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7805..7824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8618..9206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9107..9126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9360..9377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9494..9515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9530..9535,9536..9553,9554..9560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9568..9584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9573..9595
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9592..9608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9592..9608
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9627..9647
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9629..9647
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     1861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1981 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2041 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggtgtgcag gtgagtgatc
     2101 caaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     2161 ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa
     2221 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     2281 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     2341 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     2401 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     2461 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     2521 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     2581 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     2641 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     2701 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     2761 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     2821 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     2881 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     2941 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     3001 aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca
     3061 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     3121 gggcgcccca gctggcaatt ccgacggatc aagaacctgc tgacgtttaa gagctatgct
     3181 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     3241 gagtcggtgc tttttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     3301 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     3361 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     3421 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
     3481 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     3541 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     3601 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     3661 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     3721 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     3781 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     3841 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     3901 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     3961 gacctctctc cccaggggga tccgtgagtt tggggaccct tgattgttct ttctttttcg
     4021 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg
     4081 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac
     4141 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa
     4201 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta
     4261 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag
     4321 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa
     4381 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc
     4441 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg
     4501 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     4561 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg taatacgact
     4621 cactataggg cgaattgata tgtctagatt agataaaagt aaagtgatta acagcgcatt
     4681 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     4741 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     4801 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
     4861 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     4921 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
     4981 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
     5041 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
     5101 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     5161 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     5221 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gcgtacagcg gctcccggga
     5281 gttcgctagc ggtgctacta acttcagcct gctgaagcag gctggagacg tggaggagaa
     5341 ccctggacct ggtagtggaa cgcgtatggt gtctaagggc gaagagctga ttaaggagaa
     5401 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
     5461 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     5521 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
     5581 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
     5641 cacatgggag agagtcacca catacgaaga tgggggcgtg ctgaccgcta cccaggacac
     5701 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
     5761 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
     5821 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
     5881 ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa
     5941 gatgcctggc atctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
     6001 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
     6061 ggggcacaag cttaatccaa agaaaaagcg gaaagtgtga gtcgacctcg agggggggcc
     6121 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6181 aaaagggggg actggaaggg ctaattcact cccaacgaag aaaagatctg ctttttgctt
     6241 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6421 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6481 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6601 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6781 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6841 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6901 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6961 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7021 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7081 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7141 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7201 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7261 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7321 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7381 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7441 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7501 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7561 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7621 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7681 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7741 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7801 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7861 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7921 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7981 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8041 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8101 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8161 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8221 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8281 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8341 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8401 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8461 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8521 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8581 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8641 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8701 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8761 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8821 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8881 ttaccgggtt ggactcaaga cgatagttac tggataaggc gcagcggtcg ggctgaacgg
     8941 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9001 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9061 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9121 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9181 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9241 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9301 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9361 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9421 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9481 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9541 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9601 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9661 agctgcaagc ttaa
//
