LOCUS       sequence-228371-       10757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Jheng JR, Lau KS, Tang WF, Wu MS, Horng JT
  TITLE     Endoplasmic reticulum stress is induced and modulated by enterovirus
            71.
  JOURNAL   Cell Microbiol. 2010 Jun . 12(6):796-813.
  PUBMED    20070307
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Microbiol. 2010 Jun . 12(6):796-813."
COMMENT     Sequence Label: pFLAG-XBP1u-FLuc
FEATURES             Location/Qualifiers
     source          1..10757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     369..391
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     453..472
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     514..533
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        514..532
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    533..557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        573..682
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             732..1667
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             2549..2581
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             2591..2614
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2698..4347
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFRSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2761..2779)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             4354..4377
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(4467..4486)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4532..4587
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4586..4605)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      4675..4722
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4728..5154
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     regulatory      5168..5177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5174..5176,5177..5179,5180..5893)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELFN"
     primer_bind     complement(5219..5240)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5480..5499)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5827..5848
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_recomb     6311..7769
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           6399..6771
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(6428..7769)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      complement(7928..8515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8007..8026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8528..9319,9320..9388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9151..9170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_recomb     9454..10281
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             9454..9578
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(9616..10221)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        10343..10646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
       61 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
      121 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      181 caggtgagcg ggcgggacgg cccttctcct tcgggctgta attagcgctt ggtttaatga
      241 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
      301 tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
      361 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
      421 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
      481 tcattttggc aaagaattgg atcggaccga aattaatacg actcactata ggggaattgt
      541 gagcggataa caattccccg gagttaatcc gggaccttta attcaaccca acacaatata
      601 ttatagttaa ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt
      661 aaattacatt ttatttacaa tcaaaggaga tataccatgg cgatatcccg ggagctcgtg
      721 gatccgaatt catgacttcg aaagtttatg atccagaaca aaggaaacgg atgataactg
      781 gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt attaattatt
      841 atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac gcggcctctt
      901 cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt attataccag
      961 accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg ttacttgatc
     1021 attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag atcatttttg
     1081 tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat caagataaga
     1141 tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg gatgaatggc
     1201 ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa atggttttgg
     1261 agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag ttagaaccag
     1321 aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt cgtccaacat
     1381 tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt gtacaaattg
     1441 ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg tttattgaat
     1501 cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt cctaatactg
     1561 aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat gaaatgggaa
     1621 aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataagaa ttctcagatc
     1681 tcggcgcgcc tgcaggtcga cggtaccagg cctgctggct ggtaatcgcc agcaggcctt
     1741 tttatttggt accggttcga agcttgcggc cgcttaaaac agcctgtggg ttgcacccac
     1801 ccacagggcc cactgggcgc tagcactctg gtactgaggt acctttgtgc gcctgttttt
     1861 actccccttc ccccgaagta acttagaagc tgtaaatcaa cgatcaatag caggtgtggc
     1921 acaccagtca taccttgatc aagcacttct gtttccccgg actgagtatc aataggctgc
     1981 tcgcgcggct gaaggagaaa acgttcgtta cccgaccaac tacttcgaga agcttagtac
     2041 caccatgaac gaggcagggt gtttcgctca gcacaacccc agtgtagatc aggctgatga
     2101 gtcactgcaa cccccatggg cgaccatggc agtggctgcg ttggcggcct gcccatggag
     2161 aaatccatgg gacgctctaa ttctgacatg gtgtgaagag cctattgagc tagctggtag
     2221 tcctccggcc cctgaatgcg gctaatccta actgcggagc acatgctcac aaaccagtgg
     2281 gtggtgtgtc gtaacgggca actctgcagc ggaaccgact actttgggtg tccgtgtttc
     2341 cttttattcc tatattggct gcttatggtg acaatcaaaa agttgttacc atatagctat
     2401 tggattggcc atccggtgtg caacagggca attgtttacc tatttattgg ttttgtacca
     2461 ttatcactga agtctgtgat cactctcaaa ttcattttga ccctcaacac aatcaaacgc
     2521 ggccgcacag ctgtatacac gtgcaagcca gccagaactc gccccggaag accccgagga
     2581 tctcgagatg gactacaaag acgatgacga caagcttcca gtggccgggt ctgctgagtc
     2641 cgcagcactc agactacgtg cacctctgca gcaggtgcag gcccagttgt cgaattcgaa
     2701 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
     2761 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     2821 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
     2881 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     2941 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     3001 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
     3061 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
     3121 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcgg
     3181 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
     3241 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
     3301 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
     3361 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     3421 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     3481 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
     3541 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
     3601 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
     3661 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
     3721 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     3781 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     3841 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
     3901 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     3961 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
     4021 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
     4081 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
     4141 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     4201 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     4261 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     4321 aagaagggcg gaaagatcgc cgtgtaactc gagcaccacc atcaccatca ccatcactaa
     4381 gtgattaacc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     4441 tggctcacaa ataccactga gatcgatctt tttccctctg ccaaaaatta tggggacatc
     4501 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     4561 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     4621 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gtaactagca
     4681 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaaga tcgttgcagg
     4741 acaggatgtg gtgcccgatg tgactagctc tttgctgcag gccgtcctat cctctggttc
     4801 cgataagaga cccagaactc cggcccccca ccgcccaccg ccacccccat acatatgtgg
     4861 tacgcaagta agagtgcctg cgcatgcccc atgtgcccca ccaagagctt tgcatcccat
     4921 acaagtcccc aaagtggaga accgaaccaa ttcttcgcgg gcagaacaaa agcttctgca
     4981 cacgtctcca ctcgaatttg gagccggccg gcgtgtgcaa aagaggtgaa tcgaacgaaa
     5041 gacccgtgtg taaagccgcg tttccaaaat gtataaaacc gagagcatct ggccaatgtg
     5101 catcagttgt ggtcagcagc aaaatcaagt gaatcatctc agtgcaacta aaggggggag
     5161 tcgagtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5221 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5281 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5341 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     5401 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5461 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5521 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5581 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     5641 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     5701 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5761 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5821 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5881 gctgtttaat taattgatcc gggttattag tacatttatt aagcgctaga ttctgtgcgt
     5941 tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt ataatcttta
     6001 gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct gaatttaaat
     6061 attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa gggttgtttt
     6121 tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa acttgccaaa
     6181 tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg taataaaggt
     6241 tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc gttagtaatt
     6301 gactcggatc ggaggaaatt ctccttgaag tttccctggt gttcaaagta aaggagtttg
     6361 caccagacgc acctctgttc actggtccgg cgtattaaaa cacgatacat tgttattagt
     6421 acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt
     6481 ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat
     6541 tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt
     6601 ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat
     6661 tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat
     6721 tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg
     6781 tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg ttaaatagag
     6841 cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc
     6901 aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat
     6961 taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt ggaattattt
     7021 ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt aattcagaca
     7081 acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa tctactaatg
     7141 gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc ggggctggcg
     7201 gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc tcaaatgtct
     7261 ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc gtttttggtt
     7321 tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac aattgttgtc
     7381 tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg ggcggcaatt
     7441 cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg ggagaaggtg
     7501 gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc acgattgtgg
     7561 gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga ggcagcgctt
     7621 ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct gctataagca
     7681 ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg taagcaattg
     7741 tattgtaaag agattgtctc aagctcggaa cgctgcgctc ggtcgttcgg ctgcggcgag
     7801 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7861 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7921 tggcgttttt ccataggctc cgccccctga cgagcatcac aaaaatcgac gctcaagtca
     7981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8521 tcctttgtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9181 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9241 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9301 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9361 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9421 atcagggtta ttgtctcatg tccgcgcgtt tcctgcatct tttaatcaaa tcccaagatg
     9481 tgtataaacc accaaactgc caaaaaatga aaactgtcga caagctctgt ccgtttgctg
     9541 gcaactgcaa gggtctcaat cctatttgta attattgaat aataaaacaa ttataaatgt
     9601 caaatttgtt ttttattaac gatacaaacc aaacgcaaca agaacatttg tagtattatc
     9661 tataattgaa aacgcgtagt tataatcgct gaggtaatat ttaaaatcat tttcaaatga
     9721 ttcacagtta atttgcgaca atataatttt attttcacat aaactagacg ccttgtcgtc
     9781 ttcttcttcg tattccttct ctttttcatt tttctcttca taaaaattaa catagttatt
     9841 atcgtatcca tatatgtatc tatcgtatag agtaaatttt ttgttgtcat aaatatatat
     9901 gtctttttta atggggtgta tagtaccgct gcgcatagtt tttctgtaat ttacaacagt
     9961 gctattttct ggtagttctt cggagtgtgt tgctttaatt attaaattta tataatcaat
    10021 gaatttggga tcgtcggttt tgtacaatat gttgccggca tagtacgcag cttcttctag
    10081 ttcaattaca ccatttttta gcagcaccgg attaacataa ctttccaaaa tgttgtacga
    10141 accgttaaac aaaaacagtt cacctccctt ttctatacta ttgtctgcga gcagttgttt
    10201 gttgttaaaa ataacagcca ttgtaatgag acgcacaaac taatatcaca aactggaaat
    10261 gtctatcaat atatagttgc tctagttatt aatagtaatc aattacgggg tcattagttc
    10321 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    10381 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    10441 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    10501 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
    10561 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
    10621 acgtattagt catcgctatt accatgcatg gtcgaggtga gccccacgtt ctgcttcact
    10681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
    10741 tgtgcagcga tgggggc
//
