LOCUS       sequence-228382-       11624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for SpCas9-h(pICSL11023) and sgRNA_AtCas6 
            {AT5G04770}(pEPOR1CB0092)and sgRNA_AtCas6 {AT5G04770}(pEPOR1CB0103).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0067
FEATURES             Location/Qualifiers
     source          1..11624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5006..5026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5033..5035,5036..5038,5039..5752)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5078..5099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5339..5358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5686..5707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5765..6017
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        6269..6344
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(6356..6380)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        6636..6711
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(6723..6747)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6816..6840
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7149..7778
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8207..9280
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9346..9540
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9748..9852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9853..10647
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10818..11406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11307..11326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11542..11566
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggacaagaa
      901 gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga
      961 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     1021 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     1081 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     1141 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     1201 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     1261 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     1321 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     1381 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     1441 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     1501 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     1561 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     1621 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     1681 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     1741 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     1801 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     1861 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     1921 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     1981 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     2041 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     2101 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     2161 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     2221 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     2281 gatgactcgc aaatcagaag agactatcac tccctggaac ttcgaggaag tcgtggataa
     2341 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     2401 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     2461 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     2521 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     2581 caaagaagat tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     2641 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     2701 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     2761 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     2821 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     2881 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     2941 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     3001 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtctcc acgagcacat
     3061 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     3121 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     3181 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     3241 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     3301 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     3361 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
     3421 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
     3481 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     3541 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     3601 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     3661 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     3721 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtttc
     3781 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     3841 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct
     3901 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     3961 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     4021 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga
     4081 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     4141 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     4201 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     4261 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     4321 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     4381 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     4441 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     4501 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     4561 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     4621 gctcaaagga tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     4681 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     4741 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     4801 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     4861 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     4921 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     4981 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgggtt cgatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gagcttgtca
     5761 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5821 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     5881 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     5941 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6001 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagcatcttc attcttaaga
     6061 tatgaagata atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta
     6121 tatgggaaag aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa
     6181 aagtcccaca tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag
     6241 tagtgattgt cgagacgact agcacggcgt tttagagcta gaaatagcaa gttaaaataa
     6301 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tctagaccca
     6361 gctttcttgt acaaagttgg cattacgctt tacgaattcc catggggagc atcttcattc
     6421 ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc
     6481 catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat
     6541 cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag
     6601 tcgaagtagt gattgagcta catccgtatc acattgtttt agagctagaa atagcaagtt
     6661 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct
     6721 agacccagct ttcttgtaca aagttggcat tacgctcaga gaggatgcac atgtgaccga
     6781 gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     6841 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     6901 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga gtcagccgtg
     6961 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     7021 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     7081 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     7141 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     7201 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     7261 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     7321 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     7381 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     7441 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     7501 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     7561 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     7621 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     7681 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     7741 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     7801 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     7861 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     7921 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     7981 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     8041 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     8101 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     8161 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     8221 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     8281 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     8341 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc
     8401 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     8461 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     8521 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     8581 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     8641 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     8701 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     8761 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     8821 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     8881 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     8941 agctgattgg atgtaccgcg agattacaga aggcaagaac ccggacgtgc tgacggttca
     9001 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     9061 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     9121 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     9181 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     9241 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     9301 gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta
     9361 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     9421 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     9481 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     9541 actgtctggc cagcgcacag ccgaacagct gcaaaaagcg cccacatcaa ggctccgagt
     9601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9661 acaataaccc tgatagcctg tgcataactg tctggccagc gcacagccga acagctgcaa
     9721 aaagcgccca catcaaggct ccgagtgcgc ggaaccccta tttgtttatt tttctaaata
     9781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9841 aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
     9901 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
     9961 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    10021 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    10081 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    10141 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
    10201 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    10261 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    10321 gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    10381 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    10441 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    10501 aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag
    10561 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    10621 tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag
    10681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    11041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgc tcggatctgt tggaccggac
    11461 agtagtcatg gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    11521 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    11581 acaacttaat aacacattgc ggacgttttt aatgtactgg ggtt
//
