LOCUS       sequence-228389-       11625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtIPCS1 
            {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 
            {AT3G54020}(pEPOR1CB0104).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0073
FEATURES             Location/Qualifiers
     source          1..11625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             890..4993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5006..5026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5033..5035,5036..5038,5039..5752)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5078..5099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5339..5358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5686..5707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5765..6017
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        6270..6345
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(6357..6381)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        6637..6712
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(6724..6748)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6817..6841
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7150..7779
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8208..9281
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9347..9541
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9749..9853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9854..10648
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10819..11407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11308..11327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11543..11567
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggacaagaa
      901 gtactccatt gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga
      961 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     1021 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     1081 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     1141 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     1201 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     1261 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     1321 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     1381 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     1441 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     1501 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     1561 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     1621 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     1681 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     1741 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     1801 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     1861 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     1921 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     1981 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     2041 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     2101 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     2161 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     2221 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     2281 gatgactcgc aaatcagaag agactatcac tccctggaac ttcgaggaag tcgtggataa
     2341 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     2401 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     2461 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     2521 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     2581 caaagaagat tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     2641 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     2701 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     2761 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     2821 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     2881 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     2941 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     3001 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtctcc acgagcacat
     3061 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     3121 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     3181 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     3241 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     3301 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     3361 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatcata tcgtgcccca
     3421 gtcttttctc gcagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag
     3481 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     3541 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     3601 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     3661 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     3721 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtttc
     3781 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     3841 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccgcgct
     3901 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     3961 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     4021 ttttttcaag accgagatta cactggccaa tggagagatt cggaaggcac cacttatcga
     4081 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     4141 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     4201 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     4261 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     4321 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     4381 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     4441 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     4501 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     4561 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     4621 gctcaaagga tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     4681 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     4741 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     4801 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     4861 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     4921 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     4981 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgggtt cgatggtgag
     5041 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5101 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5161 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5221 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5281 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5341 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5401 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5461 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5521 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5581 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5641 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5701 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gagcttgtca
     5761 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5821 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     5881 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     5941 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     6001 atctatgtta ctagatcgac gctactagaa ttcgagctcg gagcatcttc attcttaaga
     6061 tatgaagata atcttcaaaa ggcccctggg aatctgaaag aagagaagca ggcccattta
     6121 tatgggaaag aacaatagta tttcttatat aggcccattt aagttgaaaa caatcttcaa
     6181 aagtcccaca tcgcttagat aagaaaacga agctgagttt atatacagct agagtcgaag
     6241 tagtgattgc gacagtgata gttagggccg ttttagagct agaaatagca agttaaaata
     6301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctagaccc
     6361 agctttcttg tacaaagttg gcattacgct ttacgaattc ccatggggag catcttcatt
     6421 cttaagatat gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc
     6481 ccatttatat gggaaagaac aatagtattt cttatatagg cccatttaag ttgaaaacaa
     6541 tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga
     6601 gtcgaagtag tgattgtcat gtgcgatgag aaaatcgttt tagagctaga aatagcaagt
     6661 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
     6721 tagacccagc tttcttgtac aaagttggca ttacgctcag agaggatgca catgtgaccg
     6781 agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     6841 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     6901 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg agtcagccgt
     6961 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     7021 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     7081 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     7141 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     7201 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     7261 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     7321 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     7381 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     7441 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     7501 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     7561 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     7621 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     7681 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     7741 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     7801 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     7861 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     7921 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     7981 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     8041 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     8101 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     8161 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     8221 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     8281 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     8341 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgt
     8401 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     8461 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     8521 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     8581 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     8641 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     8701 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     8761 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     8821 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     8881 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     8941 tagctgattg gatgtaccgc gagattacag aaggcaagaa cccggacgtg ctgacggttc
     9001 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     9061 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     9121 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     9181 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     9241 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     9301 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaagctt ctttcctgtg gatagcacgt
     9361 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     9421 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     9481 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     9541 aactgtctgg ccagcgcaca gccgaacagc tgcaaaaagc gcccacatca aggctccgag
     9601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9661 gacaataacc cgcctggcct gtgcataact gtctggccag cgcacagccg aacagctgca
     9721 aaaagcgccc acatcaaggc tccgagtgcg cggaacccct atttgtttat ttttctaaat
     9781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9841 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     9901 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     9961 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
    10021 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
    10081 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
    10141 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
    10201 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
    10261 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
    10321 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
    10381 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
    10441 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
    10501 caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca
    10561 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
    10621 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta
    10681 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10741 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10921 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10981 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    11101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    11161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11401 atggaaaaac gccagcaacg cggccttttt acggttcctg ctcggatctg ttggaccgga
    11461 cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    11521 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    11581 gacaacttaa taacacattg cggacgtttt taatgtactg gggtt
//
