LOCUS       sequence-228393-        4864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Tp901.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2009 to 2043)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-2
FEATURES             Location/Qualifiers
     source          1..4864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2009..2043
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             2107..2118
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2560..2577)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2867..2886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3482..3576)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(3674..3742,3743..4534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4538..4640)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(4817..4860)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtaccttaa tgtgtggatc acacaggaaa gtactagatg actaagaaag
      421 tagcaatcta tacacgagta tccactacta accaagcaga ggaaggcttc tcaattgatg
      481 agcaaattga ccgtttaaca aaatatgctg aagcaatggg gtggcaagta tctgatactt
      541 atactgatgc tggtttttca ggggccaaac ttgaacgccc agcaatgcaa agattaatca
      601 acgatatcga gaataaagct tttgatacag ttcttgtata taagctagac cgcctttcac
      661 gtagtgtaag agatactctt tatcttgtta aggatgtgtt cacaaaaaat aaaatagact
      721 ttatctcgct taatgaaagt attgatactt cttctgctat gggtagcttg tttctcacta
      781 ttctttctgc aattaatgag tttgaaagag agaatataaa agaacgcatg actatgggta
      841 aactagggcg agcgaaatct ggtaagtcta tgatgtggac taagacagct tttgggtatt
      901 accacaacag aaagacaggt atattagaaa ttgttccttt acaagctaca atagttgaac
      961 aaatattcac tgattattta tcaggaatat cacttacaaa attaagagat aaactcaatg
     1021 aatctggaca catcggtaaa gatataccgt ggtcttatcg taccctaaga caaacacttg
     1081 ataatccagt ttactgtggt tatatcaaat ttaaggacag cctatttgaa ggtatgcaca
     1141 aaccaattat cccttatgag acttatttaa aagttcaaaa agagctagaa gaaagacaac
     1201 agcagactta tgaaagaaat aacaacccta gacctttcca agctaaatat atgctgtcag
     1261 ggatggcaag gtgcggttac tgtggagcac ctttaaaaat tgttcttggc cacaaaagaa
     1321 aagatggaag ccgcactatg aaatatcact gtgcaaatag atttcctcga aaaacaaaag
     1381 gaattacagt atataatgac aataaaaagt gtgattcagg aacttatgat ttaagtaatt
     1441 tagaaaatac tgttattgac aacctgattg gatttcaaga aaataatgac tccttattga
     1501 aaattatcaa tggcaacaac caacctattc ttgatacttc gtcatttaaa aagcaaattt
     1561 cacagatcga taaaaaaata caaaagaact ctgatttgta cctaaatgat tttatcacta
     1621 tggatgagtt gaaagatcgt actgattccc ttcaggctga gaaaaagctg cttaaagcta
     1681 agattagcga aaataaattt aatgactcta ctgatgtttt tgagttagtt aaaactcagt
     1741 tgggctcaat tccgattaat gaactatcat atgataataa aaagaaaatc gtcaacaacc
     1801 ttgtatcaaa ggttgatgtt actgctgata atgtagatat catatttaaa ttccaactcg
     1861 ctaccggtta aattgaacct ctactacgag tgagttgaga ttacagcctt actcggtacc
     1921 aaattccaga aaagagacgc tttcgagcgt cttttttcgt tttggtccct tacgctatta
     1981 ttgaagccag tctgttaccg aagtgaaatt gacggctagc tcagtcctag gtacagtgct
     2041 agccagagga aggaggctcg aacgattacg gattgctcta ctgtgactga agtttacaac
     2101 ccaattattg aaggccgcta acgcggcctt tttttgtttc tggtctccct gaggcacaga
     2161 gatttacttt attcacgact tcagatacgc acagctaaca ccacgtcgtc cctatctgct
     2221 gccctaggtc tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtatgat
     2281 atattcaggg agaccacaac ggtttccctc tacaaataat tttgtttaac tttagtaatt
     2341 tcaacaaaat aactaggatt cgaaatacgg tctactacag aagggtggtt tatcttactc
     2401 aacgacgaac aataaggcct ccctaacggg gggccttttt tattgataac aaaaagattt
     2461 ccctcgtcac gcagtaagta tttatcgtag aagatcactt ttatcggttt ccagaacagg
     2521 taagagccaa tagtgattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     2581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tcctagctag
     2641 ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2701 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3121 ttatcgccac tggcagcarc ttatcgccac tggcagcagc cactggtaac aggattagca
     3181 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3241 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3301 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt ttgtttgcaa
     3361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga cttgtgcttg
     3481 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     3541 gttctgaggt cattactgga tctatcagca ggagtccaag cgagctcgat atcactaaat
     3601 acattcaaat atctatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3661 aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgccgaaga
     3781 tcagttgggt gcacgtgtgg gttacatcga actggacctc aacagcggta agattcttga
     3841 gagttttcgc cccgaagaac gtttcccaat gatgagcact tttaaagttc tgctctgtgg
     3901 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg acggcatgac
     4021 agtacgcgaa ttatgcagcg ctgccataac catgagtgat aacacggcgg ccaacttact
     4081 tctgacaacg atcggaggac cgaaggagct taccgctttt ttgcacaaca tgggtgatca
     4141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4201 tgacaccacg atgcctgtag ctatggcaac aacgttgcgc aaactcttaa ctggcgaact
     4261 tcttactctc gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4501 tgagataggt gcctccctga ttaagcattg gtaataattt agcttcctta gctcctgaaa
     4561 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     4621 aacctcttac gtgccgatca acgtctcatt ttcgccagat atcgacgtct aagaaaccat
     4681 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct
     4741 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     4801 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     4861 tgga
//
