LOCUS       sequence-228395-       10779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level2 MoClo construct, containing level1 plant expression cassettes
            for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and 
            sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Raitskin O, Schudoma C, West A, Patron NJ
  TITLE     Comparison of efficiency and specificity of CRISPR-associated (Cas) 
            nucleases in plants: An expanded toolkit for precision genome 
            engineering.
  JOURNAL   PLoS One. 2019 Feb 27;14(2):e0211598. doi: 
            10.1371/journal.pone.0211598. eCollection 2019.
  PUBMED    30811422
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-02-27"; volume: "14(2):e0211598. doi"; pages: " 
            10.1371/journal.pone.0211598. eCollection 2019"
COMMENT     Sequence Label: pEPOR2KN0076
FEATURES             Location/Qualifiers
     source          1..10779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        454..799
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     742..762
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             896..916
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4097..4144
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4151..4231
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             join(4238..4240,4241..4243,4244..4957)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4283..4304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4544..4563)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4891..4912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4970..5222
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        5475..5550
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_RNA        5817..5892
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     misc_feature    5971..5995
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6304..6933
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7362..8435
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8501..8695
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        8903..9007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9008..9802
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9973..10561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10462..10481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10697..10721
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gaacactctg tgccgatgta tcacctctcg caagaattca agcttggagg tcaacatggt
       61 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
      121 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
      181 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
      241 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
      301 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
      361 gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact ccaaaaatgt
      421 caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa ggataatttc
      481 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
      541 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
      601 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      661 agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct ccactgacgt
      721 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      781 atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca attaccaaca
      841 acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa tggccccaaa
      901 gaagaagcgg aaggtcggta tccacggagt cccagcagcc aagcggaact acatcctggg
      961 cctggacatc ggcatcacca gcgtgggcta cggcatcatc gactacgaga cacgggacgt
     1021 gatcgatgcc ggcgtgcggc tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg
     1081 gagcaagaga ggcgccagaa ggctgaagcg gcggaggcgg catagaatcc agagagtgaa
     1141 gaagctgctg ttcgactaca acctgctgac cgaccacagc gagctgagcg gcatcaaccc
     1201 ctacgaggcc agagtgaagg gcctgagcca gaagctgagc gaggaagagt tctctgccgc
     1261 cctgctgcac ctggccaaga gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac
     1321 cggcaacgag ctgtccacca aagagcagat cagccggaac agcaaggccc tggaagagaa
     1381 atacgtggcc gaactgcagc tggaacggct gaagaaagac ggcgaagtgc ggggcagcat
     1441 caacagattc aagaccagcg actacgtgaa agaagccaaa cagctgctga aggtgcagaa
     1501 ggcctaccac cagctggacc agagcttcat cgacacctac atcgacctgc tggaaacccg
     1561 gcggacctac tatgagggac ctggcgaggg cagccccttc ggctggaagg acatcaaaga
     1621 atggtacgag atgctgatgg gccactgcac ctacttcccc gaggaactgc ggagcgtgaa
     1681 gtacgcctac aacgccgacc tgtacaacgc cctgaacgac ctgaacaatc tcgtgatcac
     1741 cagggacgag aacgagaagc tggaatatta cgagaagttc cagatcatcg agaacgtgtt
     1801 caagcagaag aagaagccca ccctgaagca gatcgccaaa gaaatcctcg tgaacgaaga
     1861 ggatattaag ggctacagag tgaccagcac cggcaagccc gagttcacca acctgaaggt
     1921 gtaccacgac atcaaggaca ttaccgcccg gaaagagatt attgagaacg ccgagctgct
     1981 ggatcagatt gccaagatcc tgaccatcta ccagagcagc gaggacatcc aggaagaact
     2041 gaccaatctg aactccgagc tgacccagga agagatcgag cagatctcta atctgaaggg
     2101 ctataccggc acccacaacc tgagcctgaa ggccatcaac ctgatcctgg acgagctgtg
     2161 gcacaccaac gacaaccaga tcgctatctt caaccggctg aagctggtgc ccaagaaggt
     2221 ggacctgtcc cagcagaaag agatccccac caccctggtg gacgacttca tcctgagccc
     2281 cgtcgtgaag agaagcttca tccagagcat caaagtgatc aacgccatca tcaagaagta
     2341 cggcctgccc aacgacatca ttatcgagct ggcccgcgag aagaactcca aggacgccca
     2401 gaaaatgatc aacgagatgc agaagcggaa cgcgaaaacc aacgagcgga tcgaggaaat
     2461 catccggacc accggcaaag agaacgccaa gtacctgatc gagaagatca agctgcacga
     2521 catgcaggaa ggcaagtgcc tgtacagcct ggaagccatc cctctggaag atctgctgaa
     2581 caaccccttc aactatgagg tggaccacat catccccaga agcgtgtcct tcgacaacag
     2641 cttcaacaac aaggtgctcg tgaagcagga agaaaacagc aagaagggca accggacccc
     2701 attccagtac ctgagcagca gcgacagcaa gatcagctac gaaaccttca agaagcacat
     2761 cctgaatctg gccaagggca agggcagaat cagcaagacc aagaaagagt atctgctgga
     2821 agaacgggac atcaacaggt tctccgtgca gaaagacttc atcaaccgga acctggtgga
     2881 taccagatac gccaccgcac gtctgatgaa cctgctgcgg agctacttca gagtgaacaa
     2941 cctggacgtg aaagtgaagt ccatcaatgg cggcttcacc agctttctgc ggcggaagtg
     3001 gaagtttaag aaagagcgga acaaggggta caagcaccac gccgaggacg ccctgatcat
     3061 tgccaacgcc gatttcatct tcaaagagtg gaagaaactg gacaaggcca aaaaagtgat
     3121 ggaaaaccag atgttcgagg aaaagcaggc cgagagcatg cccgagatcg aaaccgagca
     3181 ggagtacaaa gagatcttca tcacccccca ccagatcaag cacattaagg acttcaagga
     3241 ctacaagtac agccaccggg tggacaagaa gcctaataga gagctgatta acgacaccct
     3301 gtactccacc cggaaggacg acaagggcaa caccctgatc gtgaacaatc tgaacggcct
     3361 gtacgacaag gacaatgaca agctgaaaaa gctgatcaac aagagccccg aaaagctgct
     3421 gatgtaccac cacgaccccc agacctacca gaaactgaag ctgattatgg aacagtacgg
     3481 cgacgagaag aatcccctgt acaagtacta cgaggaaacc gggaactacc tgaccaagta
     3541 ctccaaaaag gacaacggcc ccgtgatcaa gaagattaag tattacggca acaaactgaa
     3601 cgcccatctg gacatcaccg acgactaccc caacagcaga aacaaggtcg tgaagctgtc
     3661 cctgaagccc tacagattcg acgtgtacct ggacaatggc gtgtacaagt tcgtgaccgt
     3721 gaagaatctg gatgtgatca aaaaagaaaa ctactacgaa gtgaatagca agtgctatga
     3781 ggaagctaag aagctgaaga agatcagcaa ccaggccgag tttatcgcct ccttctacaa
     3841 caacgatctg atcaagatca acggcgagct gtatagagtg atcggcgtga acaacgacct
     3901 gctgaaccgg atcgaagtga acatgatcga catcacctac cgcgagtacc tggaaaacat
     3961 gaacgacaag aggcccccca ggatcattaa gacaatcgcc tccaagaccc agagcattaa
     4021 gaagtacagc acagacattc tgggcaacct gtatgaagtg aaatctaaga agcaccctca
     4081 gatcatcaaa aagggcaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa
     4141 aaagggatcc tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta
     4201 cgcttaccca tacgatgttc cagattacgc tggttcgatg gtgagcaagg gcgaggagct
     4261 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4321 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4381 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     4441 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4501 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4561 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4621 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4681 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4741 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4801 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     4861 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4921 cgggatcact ctcggcatgg acgagctgta caagtgagct tgtcaagcag atcgttcaaa
     4981 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5041 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5101 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     5161 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     5221 tcgacgctac tagaattcga gctcggagca tcttcattct taagatatga agataatctt
     5281 caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg gaaagaacaa
     5341 tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc ccacatcgct
     5401 tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg attgacgctc
     5461 gaatgaacaa cggagtttta gtactctgga aacagaatct actaaaacaa ggcaaaatgc
     5521 cgtgtttatc tcgtcaactt gttggcgaga tttttttact tgtctcgctt tacgaattcc
     5581 catggggagc atcttcattc ttaagatatg aagataatct tcaaaaggcc cctgggaatc
     5641 tgaaagaaga gaagcaggcc catttatatg ggaaagaaca atagtatttc ttatataggc
     5701 ccatttaagt tgaaaacaat cttcaaaagt cccacatcgc ttagataaga aaacgaagct
     5761 gagtttatat acagctagag tcgaagtagt gattgattac agagagatat tgagaagttt
     5821 tagtactctg gaaacagaat ctactaaaac aaggcaaaat gccgtgttta tctcgtcaac
     5881 ttgttggcga gattttttta cttgtctcgc tcagagagga tgcacatgtg accgagggac
     5941 acgaagtgat ccgtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     6001 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     6061 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggagtcag ccgtgcggct
     6121 gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc
     6181 cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt
     6241 gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa
     6301 cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca
     6361 acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat
     6421 ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc
     6481 ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta
     6541 gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac
     6601 ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg
     6661 gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg
     6721 tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac
     6781 gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc
     6841 gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg
     6901 ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca
     6961 aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt
     7021 tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg
     7081 atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat
     7141 ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt
     7201 ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa
     7261 tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg
     7321 ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat
     7381 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     7441 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     7501 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgtcgtcga
     7561 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     7621 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     7681 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     7741 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     7801 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     7861 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     7921 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     7981 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     8041 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     8101 attggatgta ccgcgagatt acagaaggca agaacccgga cgtgctgacg gttcaccccg
     8161 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     8221 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     8281 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     8341 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     8401 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     8461 ttgccctagc aggggaaaaa ggtcgaaaaa gcttctttcc tgtggatagc acgtacattg
     8521 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     8581 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     8641 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     8701 ctggccagcg cacagccgaa cagctgcaaa aagcgcccac atcaaggctc cgagtgcgcg
     8761 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8821 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaacagc tgcaaaaagc
     8881 gcccacatca aggctccgag tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8941 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9001 gaagagtatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     9061 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     9121 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     9181 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     9241 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     9301 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     9361 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     9421 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     9481 ctgggaagag gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     9541 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     9601 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     9661 gtatgacatt gccttctgcg tccggtcgct cagggaggat atcggggaag aacagtatgt
     9721 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     9781 tttactggat gaattgtttt agctgtcaga ccaagtttac tcatatatac tttagattga
     9841 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10561 aaacgccagc aacgcggcct ttttacggtt cctgctcgga tctgttggac cggacagtag
    10621 tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    10681 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    10741 ttaataacac attgcggacg tttttaatgt actggggtt
//
