LOCUS       sequence-228420-        4768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 70 to 104)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-4
FEATURES             Location/Qualifiers
     source          1..4768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..104
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             168..179
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2010..2027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2237..2824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2317..2336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2912..3006)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(3104..3172,3173..3964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3968..4070)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(4247..4290)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     RBS             4409..4420
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4475..4546
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4562..4589
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctttcgagcg tcttttttcg ttttggtccc ttacgctatt attgaagcca gtctgttacc
       61 gaagtgaaat tgacggctag ctcagtccta ggtacagtgc tagccagagg aaggaggctc
      121 gaacgattac ggattgctct actgtgactg aagtttacaa cccaattatt gaaggccgct
      181 aacgcggcct ttttttgttt ctggtctccc tgaggcacag agatttactt tattcacgac
      241 ttcagatacg cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg
      301 ttgctggata actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa
      361 cggtttccct ctacaaataa ttttgtttaa ctttagtaat ttcaacaaaa taactaggat
      421 tcgaatgaaa gtggccattt atgttcgtgt tagcaccgat gaacaggcca aagaaggttt
      481 tagcattccg gcacagcgtg aacgtctgcg tgcattttgt gcaagccagg gttgggaaat
      541 tgtgcaagaa tatattgaag aaggttggag cgcaaaagat ctggatcgtc cgcagatgca
      601 gcgtctgctg aaagatatca aaaaaggcaa cattgatatt gtgctggtgt atcgtctgga
      661 tcgcctgacc cgtagcgttc tggatctgta tctgctgtta cagacctttg aaaaatacaa
      721 tgtggcattt cgtagcgcca ccgaagttta tgataccagc accgcaatgg gtcgtctgtt
      781 tattaccctg gttgcagcac tggcacagtg ggaacgtgaa aatctggcag aacgtgttaa
      841 atttggtatc gagcagatga tcgatgaagg taaaaaaccg ggtggtcata gcccgtatgg
      901 ttacaaattt gataaagact tcaattgcac cattattgag gaagaagcag acgttgttcg
      961 tatgatctat cgcatgtatt gtgatggtta tggctatcgt agcattgcag atcgtctgaa
     1021 tgaactgatg gttaaaccgc gtattgccaa agaatggaat cataatagcg tgcgtgatat
     1081 cctgaccaac gatatctata ttggcaccta tcgttggggt gataaagttg ttccgaataa
     1141 tcatccgcct attattagcg aaaccctgtt caaaaaagcc cagaaagaaa aagaaaaacg
     1201 tggcgttgat cgtaaacgcg ttggtaaatt tctgtttacc ggtctgttac agtgtggtaa
     1261 ttgtggtggc cataaaatgc agggccattt tgataaacgt gagcagaaaa cctattaccg
     1321 ttgtaccaaa tgtcaccgca ttaccaacga aaaaaacatt ctggaaccgc tgctggatga
     1381 aattcagctg ctgattacca gcaaagaata ctttatgagc aaattcagcg accgctatga
     1441 tcagcaagag gttgttgatg ttagcgcact gacaaaagaa ctggaaaaaa tcaaacgcca
     1501 gaaagagaaa tggtacgatc tgtatatgga tgatcgtaac ccgattccga aagaagaact
     1561 gtttgccaaa attaacgaac tgaacaaaaa agaagaagaa atctatagca agctgagcga
     1621 agtggaagaa gataaagaac cggttgaaga gaaatataac cgcctgagca aaatgatcga
     1681 ttttaaacag cagtttgagc aggccaacga ctttaccaaa aaagagctgc tgttcagcat
     1741 cttcgaaaag attgtgattt atcgcgagaa aggcaagctg aaaaaaatca ccctggatta
     1801 caccctgaaa taaaatacgg tctactacag aagggtggtt tatcttactc aacgacgaac
     1861 aataaggcct ccctaacggg gggccttttt tattgataac aaaaagattt ccctcgtcac
     1921 gcagtaagta tttatcgtag aagatcactt ttatcggttt ccagaacagg taagagccaa
     1981 tagtgattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2041 cggctgcggc gagcggtatc agctcactca aaggcggtaa tcctagctag ggcgttcggc
     2101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2641 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2761 ccgctggtag cggtggtttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2881 ttaagggatt ttggtcatga cttgtgcttg gattctcacc aataaaaaac gcccggcggc
     2941 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcagca
     3001 ggagtccaag cgagctcgat atcactaaat acattcaaat atctatccgc tcatgagaca
     3061 ataaccctga taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt
     3121 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3181 aacgctggtg aaagtaaaag atgccgaaga tcagttgggt gcacgtgtgg gttacatcga
     3241 actggacctc aacagcggta agattcttga gagttttcgc cccgaagaac gtttcccaat
     3301 gatgagcact tttaaagttc tgctctgtgg cgcggtatta tcccgtattg acgccgggca
     3361 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3421 cacagaaaag catcttacgg acggcatgac agtacgcgaa ttatgcagcg ctgccataac
     3481 catgagtgat aacacggcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3541 taccgctttt ttgcacaaca tgggtgatca tgtaactcgc cttgatcgtt gggaaccgga
     3601 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag ctatggcaac
     3661 aacgttgcgc aaactcttaa ctggcgaact tcttactctc gcttcccggc aacaattaat
     3721 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3781 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3841 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3901 aactatggat gaacgaaata gacagatcgc tgagataggt gcctccctga ttaagcattg
     3961 gtaataattt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     4021 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     4081 ttcgccagat atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4141 gcgtatcacg aggccctttc gtcttcacct cgagtgccac ctgacgtcta agaaaccatt
     4201 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     4261 aaatccttag ctttcgctaa ggatgatttc tggatactgt tacttaccga ttattgtgaa
     4321 gaaccagacc gtttgttgta gcacactcgg cgaaaatctg aatagtaact tcttgacaat
     4381 taatcatccg gctcgtaatt tatgtggaaa agaggagaaa tactagataa agttgtgccg
     4441 tatccagcgg ttaccaataa tagtctccag gcatcaaata aaacgaaagg ctcagtcgaa
     4501 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     4561 tggctcacct tcgggtgggc ctttctgcgt ttatatagta aagttccaat aagactccag
     4621 gtatctgtcc gtgtaaaaaa gagtattgac ttcgcatctt tttgtaccta taatgtgtgg
     4681 atcacacagg aaagtactag attgaacctc tactacgagt gagttgagat tacagcctta
     4741 ctcggtacca aattccagaa aagagacg
//
