LOCUS       sequence-228423-        6366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Tp901.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3511 to 3545)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-5
FEATURES             Location/Qualifiers
     source          1..6366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3511..3545
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             3609..3620
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4062..4079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4369..4388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4984..5078)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(5176..5244,5245..6036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6040..6142)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6319..6362)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtacctt aatgtgtgga tcacacagga aagtactaga tgactaagaa
     1921 agtagcaatc tatacacgag tatccactac taaccaagca gaggaaggct tctcaattga
     1981 tgagcaaatt gaccgtttaa caaaatatgc tgaagcaatg gggtggcaag tatctgatac
     2041 ttatactgat gctggttttt caggggccaa acttgaacgc ccagcaatgc aaagattaat
     2101 caacgatatc gagaataaag cttttgatac agttcttgta tataagctag accgcctttc
     2161 acgtagtgta agagatactc tttatcttgt taaggatgtg ttcacaaaaa ataaaataga
     2221 ctttatctcg cttaatgaaa gtattgatac ttcttctgct atgggtagct tgtttctcac
     2281 tattctttct gcaattaatg agtttgaaag agagaatata aaagaacgca tgactatggg
     2341 taaactaggg cgagcgaaat ctggtaagtc tatgatgtgg actaagacag cttttgggta
     2401 ttaccacaac agaaagacag gtatattaga aattgttcct ttacaagcta caatagttga
     2461 acaaatattc actgattatt tatcaggaat atcacttaca aaattaagag ataaactcaa
     2521 tgaatctgga cacatcggta aagatatacc gtggtcttat cgtaccctaa gacaaacact
     2581 tgataatcca gtttactgtg gttatatcaa atttaaggac agcctatttg aaggtatgca
     2641 caaaccaatt atcccttatg agacttattt aaaagttcaa aaagagctag aagaaagaca
     2701 acagcagact tatgaaagaa ataacaaccc tagacctttc caagctaaat atatgctgtc
     2761 agggatggca aggtgcggtt actgtggagc acctttaaaa attgttcttg gccacaaaag
     2821 aaaagatgga agccgcacta tgaaatatca ctgtgcaaat agatttcctc gaaaaacaaa
     2881 aggaattaca gtatataatg acaataaaaa gtgtgattca ggaacttatg atttaagtaa
     2941 tttagaaaat actgttattg acaacctgat tggatttcaa gaaaataatg actccttatt
     3001 gaaaattatc aatggcaaca accaacctat tcttgatact tcgtcattta aaaagcaaat
     3061 ttcacagatc gataaaaaaa tacaaaagaa ctctgatttg tacctaaatg attttatcac
     3121 tatggatgag ttgaaagatc gtactgattc ccttcaggct gagaaaaagc tgcttaaagc
     3181 taagattagc gaaaataaat ttaatgactc tactgatgtt tttgagttag ttaaaactca
     3241 gttgggctca attccgatta atgaactatc atatgataat aaaaagaaaa tcgtcaacaa
     3301 ccttgtatca aaggttgatg ttactgctga taatgtagat atcatattta aattccaact
     3361 cgctaccggt taaattgaac ctctactacg agtgagttga gattacagcc ttactcggta
     3421 ccaaattcca gaaaagagac gctttcgagc gtcttttttc gttttggtcc cttacgctat
     3481 tattgaagcc agtctgttac cgaagtgaaa ttgacggcta gctcagtcct aggtacagtg
     3541 ctagccagag gaaggaggct cgaacgatta cggattgctc tactgtgact gaagtttaca
     3601 acccaattat tgaaggccgc taacgcggcc tttttttgtt tctggtctcc ctgaggcaca
     3661 gagatttact ttattcacga cttcagatac gcacagctaa caccacgtcg tccctatctg
     3721 ctgccctagg tctatgagtg gttgctggat aactttacgg gcatgcataa ggctcgtatg
     3781 atatattcag ggagaccaca acggtttccc tctacaaata attttgttta actttagtaa
     3841 tttcaacaaa ataactagga ttcgaaatac ggtctactac agaagggtgg tttatcttac
     3901 tcaacgacga acaataaggc ctccctaacg gggggccttt tttattgata acaaaaagat
     3961 ttccctcgtc acgcagtaag tatttatcgt agaagatcac ttttatcggt ttccagaaca
     4021 ggtaagagcc aatagtgatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
     4081 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatcctagct
     4141 agggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4201 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4261 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4321 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4381 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4441 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4501 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4561 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4621 acttatcgcc actggcagca gccactggta acaggaagca gccactggta acaggattag
     4681 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4741 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4801 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt ttttgtttgc
     4861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gacttgtgct
     4981 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     5041 gagttctgag gtcattactg gatctatcag caggagtcca agcgagctcg atatcactaa
     5101 atacattcaa atatctatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5161 tgaaaaagga agaatatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5221 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgccgaa
     5281 gatcagttgg gtgcacgtgt gggttacatc gaactggacc tcaacagcgg taagattctt
     5341 gagagttttc gccccgaaga acgtttccca atgatgagca cttttaaagt tctgctctgt
     5401 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5461 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggacggcatg
     5521 acagtacgcg aattatgcag cgctgccata accatgagtg ataacacggc ggccaactta
     5581 cttctgacaa cgatcggagg accgaaggag cttaccgctt ttttgcacaa catgggtgat
     5641 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5701 cgtgacacca cgatgcctgt agctatggca acaacgttgc gcaaactctt aactggcgaa
     5761 cttcttactc tcgcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5821 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5881 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5941 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6001 gctgagatag gtgcctccct gattaagcat tggtaataat ttagcttcct tagctcctga
     6061 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     6121 ggaacctctt acgtgccgat caacgtctca ttttcgccag atatcgacgt ctaagaaacc
     6181 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
     6241 ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     6301 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     6361 tctgga
//
