LOCUS       sequence-228425-        6562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Int5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2048 to 2082)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-6
FEATURES             Location/Qualifiers
     source          1..6562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2048..2082
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             3805..3816
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4258..4275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4565..4584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5180..5274)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(5372..5440,5441..6232)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6236..6338)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6515..6558)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtaccta taatgtgtgg atcacacagg aaagtactag attgaacctc
     1921 tactacgagt gagttgagat tacagcctta ctcggtacca aattccagaa aagagacgct
     1981 ttcgagcgtc ttttttcgtt ttggtccctt acgctattat tgaagccagt ctgttaccga
     2041 agtgaaattg acggctagct cagtcctagg tacagtgcta gccagaggaa ggaggctcga
     2101 tgcctggtat gaccaccgaa accggtccgg atccggcagg tctgattgac ctgttttgtc
     2161 gtaaaagcaa agcagttaaa agccgtgcaa atggtgcagg tcagcgtcgt aaacaagaaa
     2221 ttagcattgc agcacaagaa accctgggtc gtaaagttgc agcactgctg ggtatgcagg
     2281 ttcgtcatgt ttggaaagaa gttggtagcg caagccgttt tcgtaaaggt aaagcacgtg
     2341 atgatcagag caaagcactg aaagccctgg aaagcggtga agttggtgca ctgtggtgtt
     2401 atcgtctgga tcgttgggat cgtggtggtg ccggtgcaat tctgaaaatt atcgaaccgg
     2461 aagatggtat gcctcgtcgt ctgctgtttg gttgggatga agataccggt agtccggttc
     2521 tggatagcac caataaacgt gatcgcggtg aactgattcg tcgtgcagaa gaagcacgcg
     2581 aagaagcaga aaaactgagc gaacgtgttc gtgataccaa agcacatcag cgtgaaaatg
     2641 gtgaatgggt taatgcccgt gcaccgtatg gtctgcgtgt tgttctggtt accgttagtg
     2701 atgaagaggg tgatgaatat gatgaacgta aactggcagc agatgatgaa gatgcgggtg
     2761 gtcctgatgg tctgaccaaa gcagaagcag cccgtctggt ttttaccctg ccggttaccg
     2821 atcgtctgag ctatgcaggc accgcacatg caatgaatac ccgtgaaatt ccgagcccga
     2881 ccggtggtcc gtggattgca gttaccgtgc gtgatatgat tcagaatccg gcatatgcgg
     2941 gttggcagac cacaggtcgt caggatggta aacagcgtcg tctgaccttt tataacggtg
     3001 aaggtaaacg tgttagcgtt atgcatggtc ctccgctggt gaccgatgaa gaacaagaag
     3061 ccgcaaaagc agccgttaaa ggtgaagatg gtgttggtgt tccgctggat ggtagcgatc
     3121 atgatacccg tcgcaaacat ctgctgagcg gtcgtatgcg ttgtccgggt tgtggtggta
     3181 gctgtagcta tagcggtaat ggttatcgtt gttggcgtag cagtgtgaaa ggtggttgtc
     3241 cggcaccgac ctatgttgca cgtaaaagcg ttgaagaata tgttgcattt cgttgggcag
     3301 caaaattagc agcaagcgaa ccggatgatc cgtttgttat tgcagttgca gatcgctggg
     3361 cagcactgac ccatccgcag gcaagcgaag atgaaaagta tgcaaaagcc gcagttcgtg
     3421 aagccgaaaa aaatctgggt cgcctgctgc gtgatcgtca gaatggtgtt tatgatggtc
     3481 cggcagaaca gttttttgcc cctgcatatc aagaagcact gagcaccttg caggcagcca
     3541 aagatgcagt tagcgaaagc agcgcaagcg cagcagttga tgttagctgg attgttgata
     3601 gcagcgatta tgaagaactg tggctgcgtg caaccccgac catgcgtaat gcaattattg
     3661 atacctgcat cgatgaaatt tgggttgcaa aaggccagcg tggtcgtccg tttgatggtg
     3721 atgaacgcgt taaaatcaaa tgggcagccc gtacctaaaa cgattacgga ttgctctact
     3781 gtgactgaag tttacaaccc aattattgaa ggccgctaac gcggcctttt tttgtttctg
     3841 gtctccctga ggcacagaga tttactttat tcacgacttc agatacgcac agctaacacc
     3901 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact ttacgggcat
     3961 gcataaggct cgtatgatat attcagggag accacaacgg tttccctcta caaataattt
     4021 tgtttaactt tagtaatttc aacaaaataa ctaggattcg aaatacggtc tactacagaa
     4081 gggtggttta tcttactcaa cgacgaacaa taaggcctcc ctaacggggg gcctttttta
     4141 ttgataacaa aaagatttcc ctcgtcacgc agtaagtatt tatcgtagaa gatcactttt
     4201 atcggtttcc agaacaggta agagccaata gtgattactt cgcgttatgc aggcttcctc
     4261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4321 ggcggtaatc ctagctaggg cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gmagcagcca
     4861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     4921 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     4981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5041 gtggtttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5161 ggtcatgact tgtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     5221 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcagcagg agtccaagcg
     5281 agctcgatat cactaaatac attcaaatat ctatccgctc atgagacaat aaccctgata
     5341 aatgcttcaa taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct
     5401 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5461 agtaaaagat gccgaagatc agttgggtgc acgtgtgggt tacatcgaac tggacctcaa
     5521 cagcggtaag attcttgaga gttttcgccc cgaagaacgt ttcccaatga tgagcacttt
     5581 taaagttctg ctctgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5641 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5701 tcttacggac ggcatgacag tacgcgaatt atgcagcgct gccataacca tgagtgataa
     5761 cacggcggcc aacttacttc tgacaacgat cggaggaccg aaggagctta ccgctttttt
     5821 gcacaacatg ggtgatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5881 cataccaaac gacgagcgtg acaccacgat gcctgtagct atggcaacaa cgttgcgcaa
     5941 actcttaact ggcgaacttc ttactctcgc ttcccggcaa caattaatag actggatgga
     6001 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6061 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6121 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6181 acgaaataga cagatcgctg agataggtgc ctccctgatt aagcattggt aataatttag
     6241 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     6301 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccagatat
     6361 cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     6421 gccctttcgt cttcacctcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     6481 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     6541 ttcgctaagg atgatttctg ga
//
