LOCUS       sequence-228427-        6292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2048 to 2082)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-7
FEATURES             Location/Qualifiers
     source          1..6292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2048..2082
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             2146..2157
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3988..4005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4295..4314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4910..5004)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(5102..5170,5171..5962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5966..6068)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6245..6288)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtaccta taatgtgtgg atcacacagg aaagtactag attgaacctc
     1921 tactacgagt gagttgagat tacagcctta ctcggtacca aattccagaa aagagacgct
     1981 ttcgagcgtc ttttttcgtt ttggtccctt acgctattat tgaagccagt ctgttaccga
     2041 agtgaaattg acggctagct cagtcctagg tacagtgcta gccagaggaa ggaggctcga
     2101 acgattacgg attgctctac tgtgactgaa gtttacaacc caattattga aggccgctaa
     2161 cgcggccttt ttttgtttct ggtctccctg aggcacagag atttacttta ttcacgactt
     2221 cagatacgca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt
     2281 gctggataac tttacgggca tgcataaggc tcgtatgata tattcaggga gaccacaacg
     2341 gtttccctct acaaataatt ttgtttaact ttagtaattt caacaaaata actaggattc
     2401 gaatgaaagt ggccatttat gttcgtgtta gcaccgatga acaggccaaa gaaggtttta
     2461 gcattccggc acagcgtgaa cgtctgcgtg cattttgtgc aagccagggt tgggaaattg
     2521 tgcaagaata tattgaagaa ggttggagcg caaaagatct ggatcgtccg cagatgcagc
     2581 gtctgctgaa agatatcaaa aaaggcaaca ttgatattgt gctggtgtat cgtctggatc
     2641 gcctgacccg tagcgttctg gatctgtatc tgctgttaca gacctttgaa aaatacaatg
     2701 tggcatttcg tagcgccacc gaagtttatg ataccagcac cgcaatgggt cgtctgttta
     2761 ttaccctggt tgcagcactg gcacagtggg aacgtgaaaa tctggcagaa cgtgttaaat
     2821 ttggtatcga gcagatgatc gatgaaggta aaaaaccggg tggtcatagc ccgtatggtt
     2881 acaaatttga taaagacttc aattgcacca ttattgagga agaagcagac gttgttcgta
     2941 tgatctatcg catgtattgt gatggttatg gctatcgtag cattgcagat cgtctgaatg
     3001 aactgatggt taaaccgcgt attgccaaag aatggaatca taatagcgtg cgtgatatcc
     3061 tgaccaacga tatctatatt ggcacctatc gttggggtga taaagttgtt ccgaataatc
     3121 atccgcctat tattagcgaa accctgttca aaaaagccca gaaagaaaaa gaaaaacgtg
     3181 gcgttgatcg taaacgcgtt ggtaaatttc tgtttaccgg tctgttacag tgtggtaatt
     3241 gtggtggcca taaaatgcag ggccattttg ataaacgtga gcagaaaacc tattaccgtt
     3301 gtaccaaatg tcaccgcatt accaacgaaa aaaacattct ggaaccgctg ctggatgaaa
     3361 ttcagctgct gattaccagc aaagaatact ttatgagcaa attcagcgac cgctatgatc
     3421 agcaagaggt tgttgatgtt agcgcactga caaaagaact ggaaaaaatc aaacgccaga
     3481 aagagaaatg gtacgatctg tatatggatg atcgtaaccc gattccgaaa gaagaactgt
     3541 ttgccaaaat taacgaactg aacaaaaaag aagaagaaat ctatagcaag ctgagcgaag
     3601 tggaagaaga taaagaaccg gttgaagaga aatataaccg cctgagcaaa atgatcgatt
     3661 ttaaacagca gtttgagcag gccaacgact ttaccaaaaa agagctgctg ttcagcatct
     3721 tcgaaaagat tgtgatttat cgcgagaaag gcaagctgaa aaaaatcacc ctggattaca
     3781 ccctgaaata aaatacggtc tactacagaa gggtggttta tcttactcaa cgacgaacaa
     3841 taaggcctcc ctaacggggg gcctttttta ttgataacaa aaagatttcc ctcgtcacgc
     3901 agtaagtatt tatcgtagaa gatcactttt atcggtttcc agaacaggta agagccaata
     3961 gtgattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc ctagctaggg cgttcggctg
     4081 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4141 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4381 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4561 gcagcagcca ctggtaacag gaagcagcca ctggtaacag gattagcaga gcgaggtatg
     4621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt gtttgcaagc agcagattac
     4801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4861 gtggaacgaa aactcacgtt aagggatttt ggtcatgact tgtgcttgga ttctcaccaa
     4921 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     4981 ttactggatc tatcagcagg agtccaagcg agctcgatat cactaaatac attcaaatat
     5041 ctatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa
     5101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gccgaagatc agttgggtgc
     5221 acgtgtgggt tacatcgaac tggacctcaa cagcggtaag attcttgaga gttttcgccc
     5281 cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctctgtggcg cggtattatc
     5341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5401 ggttgagtac tcaccagtca cagaaaagca tcttacggac ggcatgacag tacgcgaatt
     5461 atgcagcgct gccataacca tgagtgataa cacggcggcc aacttacttc tgacaacgat
     5521 cggaggaccg aaggagctta ccgctttttt gcacaacatg ggtgatcatg taactcgcct
     5581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5641 gcctgtagct atggcaacaa cgttgcgcaa actcttaact ggcgaacttc ttactctcgc
     5701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5941 ctccctgatt aagcattggt aataatttag cttccttagc tcctgaaaat ctcgataact
     6001 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     6061 gccgatcaac gtctcatttt cgccagatat cgacgtctaa gaaaccatta ttatcatgac
     6121 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtgccacct
     6181 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6241 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg ga
//
