LOCUS       sequence-228439-        2920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing shortened version of upstream V-C CRISPR array 
            for integration assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVC-CRISPR1
FEATURES             Location/Qualifiers
     source          1..2920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1243..1831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1732..1751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1985..2002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2451..2467
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2451..2467
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2480..2536
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2537..2554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2537..2553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2546..2568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2762..2781)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2881..2903
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1381 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1921 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1981 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgctacgtgc
     2041 cgatcaacgt atcattttcg ccagatatcg acgaagagcc ttcatcacag aaagcttgag
     2101 gaagagcgtc tccggagtcc ggagacgctg caatgatggt catatacgat aaattaggga
     2161 cgcagaagac cgatccttcc cggatcaaca atggacgtct agaaagcagg tcttctttac
     2221 tttccttata aacccttcca ataatgggga gaccacgaca ggtctccctt ataaaccctt
     2281 ccaataatgg gccagagttt ggtattgatc cttataaacc cttccaataa tgggagacta
     2341 gcaaatatct atttgaaagt caattataaa agtataataa atttgtttaa tatgtgatta
     2401 aacttgatag aaaatatatt aaaaagttaa ataaatataa caatttcaca caggaaacag
     2461 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg
     2521 taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2581 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2641 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     2701 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     2761 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     2821 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2881 ccgggagctg catgtgtcag aggttttcac cgtcatcacc
//
