LOCUS       sequence-228443-        3043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing shortened version of V-D CRISPR array for 
            integration assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVD_CRISPR
FEATURES             Location/Qualifiers
     source          1..3043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..31
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    44..100
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(101..118)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(101..117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..132)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(326..345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     445..467
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(505..523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        591..695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(696..764,765..1556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(914..933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1727..2315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2216..2235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2469..2486
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca
       61 ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac
      121 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      181 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      241 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      301 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      421 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcggg
     2521 ttataccttc ttgtaactag gggcaggggt aaagatacgc ggaaacagag tggaacaggc
     2581 ggagacactc ccagtcgtgc tagacttgaa aaacagaata tgccggagtg atgagtggga
     2641 aggctagttc tctttctcgc tctcaacgga gctcaggaga atggaaccat catcattttt
     2701 ttgcggggat acaaaaggaa agagtttcgg gcttgaattg acaagcatgc ctttttccct
     2761 ctaatcttcg gggatcaata ccaaactctg ggcctttttc cctctaatct tcggggagat
     2821 aaggctagag aaaaacctgc tttaccccat aatcagaggt tccccggcat attctatttt
     2881 caaagaacaa tggaatagag cggaaaaaac cctccgtttc tatcccgttt ttgatacgtt
     2941 tcccaagaaa tgcatgtcac tgcgaaccta catcattttc taaaataaga caagcattat
     3001 ttttactaag caattaagca taccagacgc tctccatgat tat
//
