LOCUS       sequence-228447-        6269 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Helper plasmid for production of phagemid ssDNA, based on m13mp18.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz 
            H
  TITLE     Biotechnological mass production of DNA origami.
  JOURNAL   Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650.
  PUBMED    29219963
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-12-6- 6"; volume: "552"; issue: "7683"; pages:
            "84-87"
COMMENT     Sequence Label: HP4_M13
FEATURES             Location/Qualifiers
     source          1..6269
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..57)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             268..1500
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             1512..1775
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1777..1878
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1875..1973
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             1970..2191
                     /codon_start=1
                     /gene="VIII"
                     /product="pVIII"
                     /label=M13 gene VIII
                     /note="major coat protein"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      2195..2242
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             2247..3521
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKSHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDLFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             3524..3862
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             3864..4910
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             4888..6168
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      6211..6242
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccagt
       61 gagttgattg ctacgtaatt agttagttag cccttagtga ctccagacgc gaattatttt
      121 tgatggcgtt cctattggtt aaaaaatgag ctgatttaac aaaaatttaa tgcgaatttt
      181 aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct gtttttgggg
      241 cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc
      301 atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt tgtagatctc
      361 tcaaaaatag ctaccctctc cggcattaat ttatcagcta gaacggttga atatcatatt
      421 gatggtgatt tgactgtctc cggcctttct cacccttttg aatctttacc tacacattac
      481 tcaggcattg catttaaaat atatgagggt tctaaaaatt tttatccttg cgttgaaata
      541 aaggcttctc ccgcaaaagt attacagggt cataatgttt ttggtacaac cgatttagct
      601 ttatgctctg aggctttatt gcttaatttt gctaattctt tgccttgcct gtatgattta
      661 ttggatgtta atgctactac tattagtaga attgatgcca ccttttcagc tcgcgcccca
      721 aatgaaaata tagctaaaca ggttattgac catttgcgaa atgtatctaa tggtcaaact
      781 aaatctactc gttcgcagaa ttgggaatca actgttatat ggaatgaaac ttccagacac
      841 cgtactttag ttgcatattt aaaacatgtt gagctacagc attatattca gcaattaagc
      901 tctaagccat ccgcaaaaat gacctcttat caaaaggagc aattaaaggt actctctaat
      961 cctgacctgt tggagtttgc ttccggtctg gttcgctttg aagctcgaat taaaacgcga
     1021 tatttgaagt ctttcgggct tcctcttaat ctttttgatg caatccgctt tgcttctgac
     1081 tataatagtc agggtaaaga cctgattttt gatttatggt cattctcgtt ttctgaactg
     1141 tttaaagcat ttgaggggga ttcaatgaat atttatgacg attccgcagt attggacgct
     1201 atccagtcta aacattttac tattaccccc tctggcaaaa cttcttttgc aaaagcctct
     1261 cgctattttg gtttttatcg tcgtctggta aacgagggtt atgatagtgt tgctcttact
     1321 atgcctcgta attccttttg gcgttatgta tctgcattag ttgaatgtgg tattcctaaa
     1381 tctcaactga tgaatctttc tacctgtaat aatgttgttc cgttagttcg ttttattaac
     1441 gtagattttt cttcccaacg tcctgactgg tataatgagc cagttcttaa aatcgcataa
     1501 ggtaattcac aatgattaaa gttgaaatta aaccatctca agcccaattt actactcgtt
     1561 ctggtgtttc tcgtcagggc aagccttatt cactgaatga gcagctttgt tacgttgatt
     1621 tgggtaatga atatccggtt cttgtcaaga ttactcttga tgaaggtcag ccagcctatg
     1681 cgcctggtct gtacaccgtt catctgtcct ctttcaaagt tggtcagttc ggttccctta
     1741 tgattgaccg tctgcgcctc gttccggcta agtaacatgg agcaggtcgc ggatttcgac
     1801 acaatttatc aggcgatgat acaaatctcc gttgtacttt gtttcgcgct tggtataatc
     1861 gctgggggtc aaagatgagt gttttagtgt attcttttgc ctctttcgtt ttaggttggt
     1921 gccttcgtag tggcattacg tattttaccc gtttaatgga aacttcctca tgaaaaagtc
     1981 tttagtcctc aaagcctctg tagccgttgc taccctcgtt ccgatgctgt ctttcgctgc
     2041 tgagggtgac gatcccgcaa aagcggcctt taactccctg caagcctcag cgaccgaata
     2101 tatcggttat gcgtgggcga tggttgttgt cattgtcggc gcaactatcg gtatcaagct
     2161 gtttaagaaa ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg
     2221 gagccttttt tttggagatt ttcaacgtga aaaaattatt attcgcaatt cctttagttg
     2281 ttcctttcta ttctcactcc gctgaaactg ttgaaagttg tttagcaaaa tcccatacag
     2341 aaaattcatt tactaacgtc tggaaagacg acaaaacttt agatcgttac gctaactatg
     2401 agggctgtct gtggaatgct acaggcgttg tagtttgtac tggtgacgaa actcagtgtt
     2461 acggtacatg ggttcctatt gggcttgcta tccctgaaaa tgagggtggt ggctctgagg
     2521 gtggcggttc tgagggtggc ggttctgagg gtggcggtac taaacctcct gagtacggtg
     2581 atacacctat tccgggctat acttatatca accctctcga cggcacttat ccgcctggta
     2641 ctgagcaaaa ccccgctaat cctaatcctt ctcttgagga gtctcagcct cttaatactt
     2701 tcatgtttca gaataatagg ttccgaaata ggcagggggc attaactgtt tatacgggca
     2761 ctgttactca aggcactgac cccgttaaaa cttattacca gtacactcct gtatcatcaa
     2821 aagccatgta tgacgcttac tggaacggta aattcagaga ctgcgctttc cattctggct
     2881 ttaatgagga tttatttgtt tgtgaatatc aaggccaatc gtctgacctg cctcaacctc
     2941 ctgtcaatgc tggcggcggc tctggtggtg gttctggtgg cggctctgag ggtggtggct
     3001 ctgagggtgg cggttctgag ggtggcggct ctgagggagg cggttccggt ggtggctctg
     3061 gttccggtga ttttgattat gaaaagatgg caaacgctaa taagggggct atgaccgaaa
     3121 atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg
     3181 attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg
     3241 gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata
     3301 attcaccttt aatgaataat ttccgtcaat atttaccttc cctccctcaa tcggttgaat
     3361 gtcgcccttt tgtctttggc gctggtaaac catatgaatt ttctattgat tgtgacaaaa
     3421 taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt atgtatgtat
     3481 tttctacgtt tgctaacata ctgcgtaata aggagtctta atcatgccag ttcttttggg
     3541 tattccgtta ttattgcgtt tcctcggttt ccttctggta actttgttcg gctatctgct
     3601 tacttttctt aaaaagggct tcggtaagat agctattgct atttcattgt ttcttgctct
     3661 tattattggg cttaactcaa ttcttgtggg ttatctctct gatattagcg ctcaattacc
     3721 ctctgacttt gttcagggtg ttcagttaat tctcccgtct aatgcgcttc cctgttttta
     3781 tgttattctc tctgtaaagg ctgctatttt catttttgac gttaaacaaa aaatcgtttc
     3841 ttatttggat tgggataaat aatatggctg tttattttgt aactggcaaa ttaggctctg
     3901 gaaagacgct cgttagcgtt ggtaagattc aggataaaat tgtagctggg tgcaaaatag
     3961 caactaatct tgatttaagg cttcaaaacc tcccgcaagt cgggaggttc gctaaaacgc
     4021 ctcgcgttct tagaataccg gataagcctt ctatatctga tttgcttgct attgggcgcg
     4081 gtaatgattc ctacgatgaa aataaaaacg gcttgcttgt tctcgatgag tgcggtactt
     4141 ggtttaatac ccgttcttgg aatgataagg aaagacagcc gattattgat tggtttctac
     4201 atgctcgtaa attaggatgg gatattattt ttcttgttca ggacttatct attgttgata
     4261 aacaggcgcg ttctgcatta gctgaacatg ttgtttattg tcgtcgtctg gacagaatta
     4321 ctttaccttt tgtcggtact ttatattctc ttattactgg ctcgaaaatg cctctgccta
     4381 aattacatgt tggcgttgtt aaatatggcg attctcaatt aagccctact gttgagcgtt
     4441 ggctttatac tggtaagaat ttgtataacg catatgatac taaacaggct ttttctagta
     4501 attatgattc cggtgtttat tcttatttaa cgccttattt atcacacggt cggtatttca
     4561 aaccattaaa tttaggtcag aagatgaaat taactaaaat atatttgaaa aagttttctc
     4621 gcgttctttg tcttgcgatt ggatttgcat cagcatttac atatagttat ataacccaac
     4681 ctaagccgga ggttaaaaag gtagtctctc agacctatga ttttgataaa ttcactattg
     4741 actcttctca gcgtcttaat ctaagctatc gctatgtttt caaggattct aagggaaaat
     4801 taattaatag cgacgattta cagaagcaag gttattcact cacatatatt gatttatgta
     4861 ctgtttccat taaaaaaggt aattcaaatg aaattgttaa atgtaattaa ttttgttttc
     4921 ttgatgtttg tttcatcatc ttcttttgct caggtaattg aaatgaataa ttcgcctctg
     4981 cgcgattttg taacttggta ttcaaagcaa tcaggcgaat ccgttattgt ttctcccgat
     5041 gtaaaaggta ctgttactgt atattcatct gacgttaaac ctgaaaatct acgcaatttc
     5101 tttatttctg ttttacgtgc aaataatttt gatatggtag gttctaaccc ttccattatt
     5161 cagaagtata atccaaacaa tcaggattat attgatgaat tgccatcatc tgataatcag
     5221 gaatatgatg ataattccgc tccttctggt ggtttctttg ttccgcaaaa tgataatgtt
     5281 actcaaactt ttaaaattaa taacgttcgg gcaaaggatt taatacgagt tgtcgaattg
     5341 tttgtaaagt ctaatacttc taaatcctca aatgtattat ctattgacgg ctctaatcta
     5401 ttagttgtta gtgctcctaa agatatttta gataaccttc ctcaattcct ttcaactgtt
     5461 gatttgccaa ctgaccagat attgattgag ggtttgatat ttgaggttca gcaaggtgat
     5521 gctttagatt tttcatttgc tgctggctct cagcgtggca ctgttgcagg cggtgttaat
     5581 actgaccgcc tcacctctgt tttatcttct gctggtggtt cgttcggtat ttttaatggc
     5641 gatgttttag ggctatcagt tcgcgcatta aagactaata gccattcaaa aatattgtct
     5701 gtgccacgta ttcttacgct ttcaggtcag aagggttcta tctctgttgg ccagaatgtc
     5761 ccttttatta ctggtcgtgt gactggtgaa tctgccaatg taaataatcc atttcagacg
     5821 attgagcgtc aaaatgtagg tatttccatg agcgtttttc ctgttgcaat ggctggcggt
     5881 aatattgttc tggatattac cagcaaggcc gatagtttga gttcttctac tcaggcaagt
     5941 gatgttatta ctaatcaaag aagtattgct acaacggtta atttgcgtga tggacagact
     6001 cttttactcg gtggcctcac tgattataaa aacacttctc aggattctgg cgtaccgttc
     6061 ctgtctaaaa tccctttaat cggcctcctg tttagctccc gctctgattc taacgaggaa
     6121 agcacgttat acgtgctcgt caaagcaacc atagtacgcg ccctgtagca tgcaggagtc
     6181 actaagggtt agttagttag attagcagaa agtcaaaagc ctccgaccgg aggcttttga
     6241 ctaaaacttc ccttggggtt atcattggg
//
