LOCUS       sequence-228449-        6270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Helper plasmid for production of phagemid ssDNA, based on M13KO7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz 
            H
  TITLE     Biotechnological mass production of DNA origami.
  JOURNAL   Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650.
  PUBMED    29219963
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-12-6- 6"; volume: "552"; issue: "7683"; pages:
            "84-87"
COMMENT     Sequence Label: HP17_KO7
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..57)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             268..1500
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             1512..1775
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1777..1878
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1875..1973
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             1970..2191
                     /codon_start=1
                     /gene="VIII"
                     /product="pVIII"
                     /label=M13 gene VIII
                     /note="major coat protein"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      2195..2243
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             2248..3522
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFSAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             3525..3863
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             3865..4911
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             4889..6169
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSIPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      6212..6243
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccagt
       61 gagttgattg ctacgtaatt agttagttag cccttagtga ctccagacgc gaattatttt
      121 tgatggcgtt cctattggtt aaaaaatgag ctgatttaac aaaaatttaa tgcgaatttt
      181 aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct gtttttgggg
      241 cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc
      301 atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt tgtagacctc
      361 tcaaaaatag ctaccctctc cggcatgaat ttatcagcta gaacggttga atatcatatt
      421 gatggtgatt tgactgtctc cggcctttct cacccttttg aatctttacc tacacattac
      481 tcaggcattg catttaaaat atatgagggt tctaaaaatt tttatccttg cgttgaaata
      541 aaggcttctc ccgcaaaagt attacagggt cataatgttt ttggtacaac cgatttagct
      601 ttatgctctg aggctttatt gcttaatttt gctaattctt tgccttgcct gtatgattta
      661 ttggatgtta acgctactac tattagtaga attgatgcca ccttttcagc tcgcgcccca
      721 aatgaaaata tagctaaaca ggttattgac catttgcgaa atgtatctaa tggtcaaact
      781 aaatctactc gttcgcagaa ttgggaatca actgttacat ggaatgaaac ttccagacac
      841 cgtactttag ttgcatattt aaaacatgtt gagctacagc accagattca gcaattaagc
      901 tctaagccat ccgcaaaaat gacctcttat caaaaggagc aattaaaggt actctctaat
      961 cctgacctgt tggagtttgc ttccggtctg gttcgctttg aagctcgaat taaaacgcga
     1021 tatttgaagt ctttcgggct tcctcttaat ctttttgatg caatccgctt tgcttctgac
     1081 tataatagtc agggtaaaga cctgattttt gatttatggt cattctcgtt ttctgaactg
     1141 tttaaagcat ttgaggggga ttcaatgaat atttatgacg attccgcagt attggacgct
     1201 atccagtcta aacattttac tattaccccc tctggcaaaa cttcttttgc aaaagcctct
     1261 cgctattttg gtttttatcg tcgtctggta aacgagggtt atgatagtgt tgctcttact
     1321 atgcctcgta attccttttg gcgttatgta tctgcattag ttgaatgtgg tattcctaaa
     1381 tctcaactga tgaatctttc tacctgtaat aatgttgttc cgttagttcg ttttattaac
     1441 gtagattttt cttcccaacg tcctgactgg tataatgagc cagttcttaa aatcgcataa
     1501 ggtaattcac aatgattaaa gttgaaatta aaccatctca agcccaattt actactcgtt
     1561 ctggtgtttc tcgtcagggc aagccttatt cactgaatga gcagctttgt tacgttgatt
     1621 tgggtaatga atatccggtt cttgtcaaga ttactcttga tgaaggtcag ccagcctatg
     1681 cgcctggtct gtacaccgtt catctgtcct ctttcaaagt tggtcagttc ggttccctta
     1741 tgattgaccg tctgcgcctc gttccggcta agtaacatgg agcaggtcgc ggatttcgac
     1801 acaatttatc aggcgatgat acaaatctcc gttgtacttt gtttcgcgct tggtataatc
     1861 gctgggggtc aaagatgagt gttttagtgt attctttcgc ctctttcgtt ttaggttggt
     1921 gccttcgtag tggcattacg tattttaccc gtttaatgga aacttcctca tgaaaaagtc
     1981 tttagtcctc aaagcctctg tagccgttgc taccctcgtt ccgatgctgt ctttcgctgc
     2041 tgagggtgac gatcccgcaa aagcggcctt taactccctg caagcctcag cgaccgaata
     2101 tatcggttat gcgtgggcga tggttgttgt cattgtcggc gcaactatcg gtatcaagct
     2161 gtttaagaaa ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg
     2221 gagccttttt ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt
     2281 gttcctttct attctcactc cgctgaaact gttgaaagtt gtttagcaaa accccataca
     2341 gaaaattcat ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat
     2401 gagggttgtc tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt
     2461 tacggtacat gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag
     2521 ggtggcggtt ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt
     2581 gatacaccta ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt
     2641 actgagcaaa accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact
     2701 ttcatgtttc agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc
     2761 actgttactc aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca
     2821 aaagccatgt atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc
     2881 tttaatgagg atccattcgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct
     2941 cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc
     3001 tctgagggtg gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct
     3061 ggttccggtg attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa
     3121 aatgccgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact
     3181 gattacggtg ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat
     3241 ggtgctactg gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat
     3301 aattcacctt taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa
     3361 tgtcgccctt ttgtctttag cgctggtaaa ccatatgaat tttctattga ttgtgacaaa
     3421 ataaacttat tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta
     3481 ttttctacgt ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg
     3541 gtattccgtt attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc
     3601 ttacttttct taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc
     3661 ttattattgg gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac
     3721 cctctgactt tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt
     3781 atgttattct ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt
     3841 cttatttgga ttgggataaa taatatggct gtttattttg taactggcaa attaggctct
     3901 ggaaagacgc tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata
     3961 gcaactaatc ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg
     4021 cctcgcgttc ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc
     4081 ggtaatgatt cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact
     4141 tggtttaata cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta
     4201 catgctcgta aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat
     4261 aaacaggcgc gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt
     4321 actttacctt ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct
     4381 aaattacatg ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt
     4441 tggctttata ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt
     4501 aattatgatt ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc
     4561 aaaccattaa atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct
     4621 cgcgttcttt gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa
     4681 cctaagccgg aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt
     4741 gactcttctc agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa
     4801 ttaattaata gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt
     4861 actgtttcca ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt
     4921 cttgatgttt gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct
     4981 gcgcgatttt gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga
     5041 tgtaaaaggt actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt
     5101 ctttatttct gttttacgtg ctaataattt tgatatggtt ggttcaattc cttccataat
     5161 tcagaagtat aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca
     5221 ggaatatgat gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt
     5281 tactcaaact tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt
     5341 gtttgtaaag tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct
     5401 attagttgtt agtgcaccta aagatatttt agataacctt cctcaattcc tttctactgt
     5461 tgatttgcca actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga
     5521 tgctttagat ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa
     5581 tactgaccgc ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg
     5641 cgatgtttta gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc
     5701 tgtgccacgt attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt
     5761 cccttttatt actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac
     5821 gattgagcgt caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg
     5881 taatattgtt ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag
     5941 tgatgttatt actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac
     6001 tcttttactc ggtggcctca ctgattataa aaacacttct caagattctg gcgtaccgtt
     6061 cctgtctaaa atccctttaa tcggcctcct gtttagctcc cgctctgatt ccaacgagga
     6121 aagcacgtta tacgtgctcg tcaaagcaac catagtacgc gccctgtagc atgcaggagt
     6181 cactaagggt tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg
     6241 actaaaactt cccttggggt tatcattggg
//
